Enhancement of gamma-linolenic acid production by the fungus Mucor sp LB-54 by growth temperature

Abstract

As a relatively prolific producer of GLA, the strain of Mucor sp LB-54 was selected for a study at different growth temperatures in shaker flask culture. The strain used in our experiment was capable to accumulate a relatively high amount of intracellular lipid, 20.73 % of dry cell weight, and GLA content of 15 % of total fatty acids after 5 days of incubation at 28°C. As the growth temperature was decreased from 28 to 12°C the percentage of GLA increased from 15 to 24 % of total fatty acids. In order to optimize the culture conditions for rapid biomass production and lipid production with a high proportion of GLA, the fungus was grown at two temperature combinations associated supplies of carbon source (glucose) in the culture medium. Maximal production of GLA (74 mg/l) was obtained from the Mucor sp LB-54 strain after 5 days of incubation at 28°C in basal medium following glucose addition (7 % w/v) and incubation for an additional 3 days at 12°C. The identity of GLA found in the strain of Mucor sp LB-54 was confirmed by the coupled gas chromatography-mass spectrometry

Antibacterial potential of γ-linolenic acid from Fischerella sp. colonizing Neem tree bark

“…Interestingly, a comparable level of GLA i.e. 15%, of total fatty acid, is also reported in Mucor (Carvalho et al 1999). In the evening primrose (Oenothera spp.…”

“…In spite of low productivity, long life cycle and the need of large areas for cultivation, seed oils from the evening primrose (Oenothera biennis) and borage (Borago officinalis) remained the commercial sources of GLA (Phillips & Huang 1996;Carvalho et al 1999). GLA is also reported in fungi such as Mortierella ramanniana (Hansson & Dosta´lek 1988), Mucor sp.…”

Culture Conditions stimulating high γ-Linolenic Acid accumulation by Spirulina platensis

“…Nevertheless, owing to their larger area of requirement for cultivation and unpredictable yields, alternative microbial sources have been targeted (5). Several species of fungi (mucor) are known to produce GLA (3,18). However, due to the presence of other polyunsaturated fatty acids in fungal oils, extensive and laborious purification steps are required before arriving at pure fatty acid.…”

Optimization of Process Parameters for the Production of Gamma-Linolenic Acid from Cunnighamella elegans CFR C07 under Submerged Fermentation

“…Studies by Shrivastava et al (7) and Ahmed et al (9) reported glucose as the major carbon source for the production of GLA. Previous studies showed an increased yield of GLA after the addition of glucose (15). After staining with Sudan black B, the presence of blue or greyish oil globules was observed within the mycelium under microscope (Fig.…”