Microbial Activity of Dark Red Latosol Samples Stored at Different Temperatures

Abstract

The enzymatic activity of soil samples stored at temperatures of 5 to -12oC and at room temperature for 0-32 weeks was determined. While alkaline phosphatase and dehydrogenase activity was decreased compared to control in samples stored at low temperatures, acid phosphatase activity showed no significant change.<br>Foi determinada a atividade enzimática de amostras de solo armazenadas às temperaturas de 5, -12oC e ambiente durante 0-32 semanas. A atividade da fosfatase alcalina e da desidrogenase diminuiu nas amostras armazenadas a baixas temperaturas, enquanto que a atividade da fosfatase ácida não variou em relação ao controle

Limitations and Potential of in situ Rhizobox Sampling for Assessing Microbial Activity in Fruit Tree Rhizosphere

“…It has been suggested by some researchers that enzyme activity is not affected by quick freezing (Asmar et al, 1995;Kandeler et al, 2002). On the other hand, Vieira and Nahas (1998) have shown that alakaline phosphatase and dehydrogenase activity were substantially reduced even during storage at )12°C. Nevertheless, it is widely accepted that freezing is a non-destructive procedure for molecular analyses, which allows looking more closely at gradients of microbial species or communities that are enriched or depleted across the rhizosphere.…”