Microbial Counts of Dark Red Latosol Samples Stored at Different Temperatures

Vieira, Francisco Cleber Sousa; Nahas, Ely

doi:10.1590/s0001-37141998000300001

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…Our results support previous research on soil bacteria [31] and forest soil fungi [32,33], suggesting that varying soil storage time and the temperature is a robust method to increase isolate diversity from most sediment samples. In addition, our study demonstrates that culture-based cave diversity metrics can be altered by logistical constraints if samples are collected and stored for long periods of time.…”

Section: Discussionsupporting

confidence: 90%

“…Another reported reason why storing soil samples above 0 • C for a prolonged duration would benefit obtaining additional species is that fungi are still metabolically active at these temperatures [33]. Active, fast-growing fungi are likely to deplete sediment carbon resources resulting in an increased proportion of slower-growing oligotrophic fungi over time.…”

Section: Discussionmentioning

confidence: 99%

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Preliminary Study of Cave Sample Storage Conditions on Fungal Community Diversity

et al. 2021

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We investigated the effect of varying storage time and storage temperature on fungal species’ isolation as part of a case study of Illinois cave sediment samples. A deeper understanding of cave fungal communities may influence eco-epidemiology studies of emerging or re-emerging cave fungal pathogens. Using culture-dependent techniques, we isolated geophilic fungi from homogeneous cave sediment samples from three Illinois caves. Each sample was stored under five different temperatures ranging from −80 °C to 22 °C. Cave sediment was periodically removed at five different time periods from 48 h to 1 year, serially diluted with distilled water, lawn plated onto two different media, and monitored for fungal colonies. We isolated colonies and confirmed identity through nrDNA sequence similarity. Our results suggest that storage time was more important than storage temperature for the isolation of a wide diversity of geophilic fungal taxa. Importantly, our results show that varying storage conditions will alter both the kind of taxa and abundance of those taxa, suggesting that comparative studies of fungal diversity across studies should employ similar storage conditions. Lastly, future investigations should utilize multiple genetic markers because the fungal barcode region lacked species-level resolution for many isolates within common Illinois geophilic fungal genera.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Preliminary Study of Cave Sample Storage Conditions on Fungal Community Diversity

et al. 2021

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“…Podese inferir essas transformações pela quantificação do número de microrganismos ou por sua atividade (NANNIPIERI et al, 1978;VIEIRA e NAHAS, 1998). Pelas transformações dos compostos fosfatados, por meio de reações de solubilização do fósforo inorgânico e de mineralização do fósforo orgânico, forma-se fosfato solúvel que pode ser utilizado pelas plantas para seu crescimento (BERTON et al, 1997).…”

Section: Introductionunclassified

Microrganismos do solo produtores de fosfatases em diferentes sistemas agrícolas

Nahas

2002

Bragantia

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RESUMOConsiderando-se que uma forma de prover fosfato disponível para as plantas é através da atividade mineralizadora microbiana do fósforo orgânico, avaliou-se a influência da planta (braquiária, guandu e sem planta), dos fertilizantes (superfosfato simples, fosfato de rocha e sem adubo) e da calagem (com e sem calcário) nas populações de microrganismos produtores de fosfatases ácida e alcalina. Do total de bactérias, 70,6% apresentaram atividade de fosfatase alcalina e 58,2% de fosfatase ácida e dos fungos, 64,3% e 84,7% respectivamente. Esses dados mostram número significativo de microrganismos com habilidade de mineralização do fósforo orgânico. Observou-se efeito restritivo do guandu sobre as bactérias e fungos produtores de fosfatases alcalina e ácida, cujos números foram sempre inferiores aos obtidos com a cultura de braquiária ou com as parcelas sem cultivo. Maior número de bactérias produtoras de fosfatase alcalina foi obtido nos tratamentos com superfosfato e controle do que com fosfato de rocha. Ao contrário, para os fungos, encontrou-se maior número nas parcelas não fertilizadas que as adubadas com fosfato. O número de fungos com atividade de fosfatase ácida diminuiu por efeito da calagem, enquanto o das bactérias aumentou. Finalmente, o número de bactérias produtoras de fosfatase alcalina superou o de fungos. Palavras-chave: bactérias, fungos, fosfatase ácida, fosfatase alcalina, fosfato de rocha, superfosfato, braquiária, guandu, calagem. ABSTRACT SOIL MICROORGANISMS PHOSPHATASE PRODUCERS IN DIFFERENT AGRICULTURAL SYSTEMSConsidering that one way for providing available phosphate to plants is by the microbial activity for the mineralization of organic phosphorus, the influence of plants (Brachiaria ruziziensis, Cajanus cajan and control), fertilizers (superphosphate, rock phosphate and control) and liming (with and without lime) was evaluated in the microorganisms populations of the acid and alkaline phosphatase producers. Among bacteria, 70,6% showed alkaline phosphatase activity and 58,2% acid phosphatase activity and among fungi, 64,3% and 84,7%, respectively. These numbers show that a significant population of microorganisms shows ability for organic phosphorus mineralization. Restrictive effect of the C. cajan was observed on the bacteria and fungi producers of the alkaline and acid phosphatases whose numbers always were lower to those obtained with the B. ruziziensis or with the plots without cultivation. Larger number of alkaline phosphatase bacterial producers was obtained in the treatments with superphosphate and control than that with rock phosphate. On the opposite, for fungi, a larger number was found in the plots with no fertilizer compared to the ones with fertilizer. The population of fungi having acid phosphatase activity decreased in the limed plots whereas the bacterial population increased. Finally, the alkaline phosphatase bacterial producers showed higher enzymatic activity than those of the fungi.

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Desenvolvimento e qualidade nutricional de mudas de mangabeiras cultivadas em substratos contendo fibra de coco e adubação fosfatada

et al. 2009

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RESUMO-Para a produção de mudas de qualidade, é necessária a utilização de substratos com propriedades físicas, químicas e biológicas que condicionem a germinação adequada das sementes e o estabelecimento das mudas. Neste sentido, foi realizado um experimento no Centro de Ciências Agrárias, da Universidade Federal da Paraíba, com o objetivo de avaliar o crescimento e a composição mineral de mudas de mangabeiras (Hancornia speciosa) em substratos compostos por diferentes proporções de fibra de coco (0% a 40%), esterco bovino (0% a 25%), terra vegetal (25% a 70%) e 15% de areia, fertilizados com superfosfato triplo (0; 5,5 e 11 g dm -3 ). A terra vegetal e a fibra de coco exerceram efeitos benéficos às mudas com o aumento de suas proporções no substrato. A adição do esterco e do superfosfato triplo inibiu a produção de matéria seca e a área foliar das mudas. Houve aumento dos teores de nutrientes nas mudas com o aumento da concentração de esterco nos substratos. Pela estimativa dos resultados, o substrato que proporciona maior crescimento e composição mineral mais equilibrada nas mudas de mangabeira deve ser constituído por 14% de esterco, 56% de terra vegetal, 15% de fibra de coco, 15% de areia e 4 g dm -3 de superfosfato triplo. Termos para indexação: Hancornia speciosa, misturas, nutrição mineral. DEVELOPMENT AND NUTRITIONAL QUALITY OF HANCORNIA SPECIOSA SEEDLINGS CULTIVATED IN MIXTURE CONTAINING COCONUT FIBER AND FERTILIZED WITH PHOSPHORUSABSTRACT -In order to produce good quality seedlings it is necessary to use a mixture that presents appropriate physical, chemical and biological properties, which supplies the necessary conditions for the germination and the seedling's establishment. This experiment was carried out at the Agricultural Sciences Center, Paraíba Federal University (Brazil), whose objective was to evaluate the growth and the mineral composition of Hancornia speciosa seedlings in substrata composed by concentrations of coconut fiber from 0% to 40%, manure bovine from 0% to 25%, soil from 25% to 70%, sand 15% and triple superphosphate between 0 and 11 g dm -3 . The soil and coconut fiber had beneficial effects on the H. speciosa seedlings with the increase of its concentrations in the mixture. The addition of the manure and the doses of triple superphosphate provoked a decrease in the matter and in the foliate area in the H. speciosa seedlings. There was an increase of the nutrients content in the seedlings in which there was an increase of the manure concentration in the mixture. It is recommended to use the concentrations of 14% of manure, 56% of soil, 15% of coconut fiber, 15% of sand and 4 g dm -3 of triple superphosphate, for the maximum estimated values of growth and nutritional composition.

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Microbial Counts of Dark Red Latosol Samples Stored at Different Temperatures

Cited by 3 publications

References 13 publications

Preliminary Study of Cave Sample Storage Conditions on Fungal Community Diversity

Preliminary Study of Cave Sample Storage Conditions on Fungal Community Diversity

Microrganismos do solo produtores de fosfatases em diferentes sistemas agrícolas

Desenvolvimento e qualidade nutricional de mudas de mangabeiras cultivadas em substratos contendo fibra de coco e adubação fosfatada

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