Shen-fu injection alleviates acute renal injury by reducing cytokine levels and modulating apoptosis in a porcine hemorrhagic shock model

Abstract

Purpose Shen-fu injection (SFI) was used to intervene in the resuscitation of porcine hemorrhagic shock (HS) model to study its protective effects on acute kidney injury. Methods After 60 min of HS, 28 animals were randomly assigned into four groups. The groups were as follows: hemorrhagic shock group (HS); HS resuscitation with shed-blood group (HSR); HS resuscitation with shed-blood and SFI (1 mL·kg –1 ) group (HSR-SFI); and the sham operation group (Sham). The bloods were analyzed for serum creatinine (sCr), cystatin C (CysC) and neutrophil gelatinase-associated lipocalin (NGAL). BAX, Bcl-2, and caspase-3 protein expressions by Western blot analysis and immunohistochemical staining. The renal tissues were removed and pathologic changes were observed. Results Mean aortic pressure (MAP) in HSR-SFI groups were higher than that in HSR groups after shock. At the 6th hour after shock, the urine volume per hour in the HSR-SFI groups was more than that in the HSR groups. The sCr, NGAL, CysC and cytokine levels of HSR-SFI groups were lower. The Bcl-2 expression was increased in the HSR-SFI groups. The BAX and caspase-3 expressions were reduced. The histopathologic score in the HSR-SFI was lower. Conclusions SFI may reduce the risk of acute kidney injury (AKI) following hemorrhagic shock by attenuating systemic inflammatory responses, and regulating the expression of apoptosis-related proteins.

The role of lncRNA HCG18 in human diseases

“…176 Research shows that overexpression of Bcl-2 can inhibit apoptosis and alleviate AKI. 177 HCG18 promotes autophagy in renal ischemia-reperfusion injury mouse model and alleviates AKI by targeting the miR-16-5p/Bcl-2 axis. 58 In summary, HCG18 provides a new idea for the treatment of AKT.…”

“…Bcl‐2 protein plays an important role in regulating important metabolic functions of kidney cells, including autophagy, metabolism, and mitochondrial morphology 176 . Research shows that overexpression of Bcl‐2 can inhibit apoptosis and alleviate AKI 177 . HCG18 promotes autophagy in renal ischemia‐reperfusion injury mouse model and alleviates AKI by targeting the miR‐16‐5p/Bcl‐2 axis 58 .…”