Effects of Rehmannia glutinosa polysaccharides on bone tissue structure and skeletal muscle atrophy in rats with disuse

Ou, Li; Kang, W.; Zhang, Jiahao; Liang, Zhijian; Li, Min; Gao, Feng; Chen, Lin

doi:10.1590/acb360403

Cited by 16 publications

(8 citation statements)

References 19 publications

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“…[18] It also plays an antioxidative role in improving the bone structure of osteoporosis rats by improving muscle atrophy. [19] The components of the BHDH Decoction were identified using HERB. At present, most studies take OB ≥ 30% and DL ≥ 0.18 as the criteria for screening ingredients, which is not comprehensive.…”

Section: Discussionmentioning

confidence: 99%

Study on the mechanism of Baihe Dihuang decoction in treating menopausal syndrome based on network pharmacology

Tian

Yang

et al. 2023

Medicine

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Menopausal syndrome (MS) refers to a series of symptoms with autonomic nervous system dysfunction caused by decreased sex hormones before and after menopause. Baihe Dihuang (BHDH) decoction positively affects MS, but its mechanism remains unclear. This study aimed to reveal the underlying mechanism through network pharmacology. The components of the BHDH Decoction were found through HERB, while corresponding targets were obtained from the HERB, Drug Bank, NPASS, Targetnet, and Swisstarget databases. The MS targets were obtained from GeneCards and OMIM. STRING was used to construct the protein-protein interaction networks. OmicShare tools were used for Gene Ontology and Kyoto encyclopedia of genes and genomes analyses. Finally, Autodock Vina 1.1.2 software (https://vina.scripps.edu/downloads/) was used for molecular alignment to verify whether the main active ingredients and key targets had good binding activity. We screened out 27 active ingredients and 251 effective targets of BHDH Decoction, 3405 MS-related targets, and 133 intersection targets between BHDH Decoction and MS. Protein-protein interaction network identified tumor protein P53, Serine/threonine-protein kinase AKT, epidermal growth factor receptor, Estrogen Receptor 1, and jun proto-oncogene as critical targets. Gene ontology analysis showed that these targets were mainly involved in the cellular response to chemical stimulus, response to oxygen-containing compound, cellular response to endogenous stimulus, response to an organic substance, and response to chemical, etc. Kyoto encyclopedia of genes and genomes pathways were mainly enriched in endocrine resistance, pathways in cancer, and the ErbB signaling pathway, etc. Molecular docking results showed that emodin and stigmasterol are strongly associated with Serine/threonine-protein kinase AKT, Estrogen Receptor 1, epidermal growth factor receptor, sarcoma gene, and tumor protein P53. This study preliminarily revealed the multi-component, multi-target, and multi-channel mechanism of BHDH Decoction in treating MS. It provides a reference for in vitro and in vivo research and clinical application of BHDH Decoction in the treatment of MS.Abbreviations: AKT1 = serine/threonine-protein kinase AKT, BC = betweenness centrality, BHDH = Baihe Dihuang, CC = closeness centrality, DL = drug-like, EGFR = epidermal growth factor receptor, ESR1 = estrogen receptor 1, GO = gene ontology, JUN = jun proto-oncogene, KEGG = Kyoto encyclopedia of genes and genomes, MS = menopausal syndrome, OB = oral bioavailability, PPI = protein-protein interaction, SRC = sarcoma gene, TP53 = tumor protein P53.

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Section: Discussionmentioning

confidence: 99%

Study on the mechanism of Baihe Dihuang decoction in treating menopausal syndrome based on network pharmacology

Tian

Yang

et al. 2023

Medicine

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“…Disuse-induced osteoporosis (DIO), which is a type of secondary osteoporosis with presently unsatisfactory treatment options, is a common complication caused by the lack, or disuse, of mechanical loading or systemic immobilization and constitutes a state of bone loss ( Yang et al., 2020 ; Rolvien and Amling, 2022 ). Currently, drug therapy remains the primary treatment for osteoporosis ( Ou et al., 2021 ). However, the long-term use of these drugs can lead to serious complications, including kidney damage, venous thrombosis, and an increased risk of developing tumors ( Ou et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, drug therapy remains the primary treatment for osteoporosis ( Ou et al., 2021 ). However, the long-term use of these drugs can lead to serious complications, including kidney damage, venous thrombosis, and an increased risk of developing tumors ( Ou et al., 2021 ). Thus, more effective, safe, and novel treatment strategies are urgently required for DIO.…”

Section: Introductionmentioning

confidence: 99%

Gut microbiota and fecal metabolic signatures in rat models of disuse-induced osteoporosis

Qiao

Zhang²,

Li³

et al. 2022

Front. Cell. Infect. Microbiol.

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BackgroundAssessing the correlation between gut microbiota (GM) and bone homeostasis has increasingly attracted research interest. Meanwhile, GM dysbiosis has been found to be associated with abnormal bone metabolism. However, the function of GM in disuse-induced osteoporosis (DIO) remains poorly understood. In our research, we evaluated the characteristics of GM and fecal metabolomics to explore their potential correlations with DIO pathogenesis.MethodsDIO rat models and controls (CON) underwent micro-CT, histological analyses, and three-point bending tests; subsequently, bone microstructures and strength were observed. ELISAs were applied for the measurement of the biochemical markers of bone turnover while GM abundance was observed using 16S rDNA sequencing. Metabolomic analyses were used to analyze alterations fecal metabolites. The potential correlations between GM, metabolites, and bone loss were then assessed.ResultsIn the DIO group, the abundance of GM was significantly altered compared to that in the CON group. Moreover, DIO significantly altered fecal metabolites. More specifically, an abnormally active pathway associated with bile acid metabolism, as well as differential bacterial genera related to bone/tissue volume (BV/TV), were identified. Lithocholic acid, which is the main secondary bile acid produced by intestinal bacteria, was then found to have a relationship with multiple differential bacterial genera. Alterations in the intestinal flora and metabolites in feces, therefore, may be responsible for DIO-induced bone loss.ConclusionsThe results indicated that changes in the abundance of GM abundance and fecal metabolites were correlated with DIO-induced bone loss, which might provide new insights into the DIO pathogenesis. The detailed regulatory role of GM and metabolites in DIO-induced bone loss needs to be explored further.

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“…Previous studies have shown that it has anti-inflammatory and antioxidative stress effects, enhances immunity and protects cardiovascular function. 20,21 However, there are no studies on the protective effects of RGP on the intestine; therefore, we aimed to investigate the protective effects of RGP on the intestine of dextran sodium sulfate IBD mice and the underlying mechanisms, including altering the intestinal microbiota, thereby repairing intestinal epithelial damage. To the best of our knowledge, the present study provides the first comprehensive intestinal evidence that RGP can effectively modulate gut microbiota and prebiotic integrative responses for the treatment of DSS-induced colitis.…”

Section: Introductionmentioning

confidence: 99%

“…Rehmannia glutinosa polysaccharide (RGP) is an important active component of the traditional Chinese herbal medicine rehmannia glutinosa, which has a wide range of pharmacological activities. Previous studies have shown that it has anti‐inflammatory and antioxidative stress effects, enhances immunity and protects cardiovascular function 20,21 . However, there are no studies on the protective effects of RGP on the intestine; therefore, we aimed to investigate the protective effects of RGP on the intestine of dextran sodium sulfate IBD mice and the underlying mechanisms, including altering the intestinal microbiota, thereby repairing intestinal epithelial damage.…”

Section: Introductionmentioning

confidence: 99%

Rehmannia glutinosa polysaccharides attenuates colitis via reshaping gut microbiota and short‐chain fatty acid production

Dong

Cai

et al. 2022

J Sci Food Agric

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BACKGROUND Ulcerative colitis is a gastrointestinal disease closely related to intestinal epithelial barrier damage and intestinal microbiome imbalance; however, effective treatment methods are currently limited. Rehmannia glutinosa polysaccharide (RGP) is an important active ingredient with a wide range of pharmacological activities, although its protective effect on colitis remains to be explored. In the present study, we verified the in vitro anti‐inflammatory effect of RGP, and observed the ameliorating effect of RGP on dextran sulfate sodium‐induced colitis in mice. RESULTS The results showed that (i) RGP attenuates lipopolysaccharide‐induced overexpression of inflammatory factors in RAW264.7 cells; (ii) RGP improves the pathological damage caused by DSS, including weight loss, increased disease activity index and intestinal tissue ulcers; (iii) RGP improves tight junction proteins to protects the tightness of the intestinal epithelium; (iv) RGP inhibits the expression of inflammatory factors through the nuclear factor‐kappa B pathway, and improved the of intestinal tissues inflammation; and (v) RGP can maintain the species diversity of intestinal microbes, increase the content of short‐chain fatty acids and then restore the imbalance of intestinal microecology. CONCLUSION RGP can improve the intestinal microbiota to strengthen the intestinal epithelial barrier and protect against DSS‐induced colitis. © 2022 Society of Chemical Industry.

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Effects of Rehmannia glutinosa polysaccharides on bone tissue structure and skeletal muscle atrophy in rats with disuse

Cited by 16 publications

References 19 publications

Study on the mechanism of Baihe Dihuang decoction in treating menopausal syndrome based on network pharmacology

Study on the mechanism of Baihe Dihuang decoction in treating menopausal syndrome based on network pharmacology

Gut microbiota and fecal metabolic signatures in rat models of disuse-induced osteoporosis

Rehmannia glutinosa polysaccharides attenuates colitis via reshaping gut microbiota and short‐chain fatty acid production

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