Gut microbiota and fecal metabolic signatures in rat models of disuse-induced osteoporosis

Qiao, Xiaochen; Zhang, Kun; Li, Xiaoyan; Li, Zhi; Wei, Wenhao; Zhou, Ruhao; Liu, Yan; Pan, Yongchun; Yang, Sen; Sun, Xiaojuan; Li, Pengcui; Xu, Chaojian; Feng, Yong; Tian, Zhi

doi:10.3389/fcimb.2022.1018897

Cited by 8 publications

(7 citation statements)

References 60 publications

(69 reference statements)

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“…The cetyltrimethylammonium ammonium bromide (CTBA) method was used to extract total DNA from all samples. Polymerase chain reaction (PCR) amplification of the V3–V4 region of the 16S rRNA gene was performed using the 341F (5`-CCTACGGGNGGCWGCAG-3`) and 805R (5`-GACTACHVGGGTATCTAATCC-3`) primers [ 26 ]. The PCR was performed as follows: initial denaturation at 98° C for 30 s, 32 denaturation cycles at 98° C for 10 s, annealing at 54° C for 30 s, extension at 72° C for 45 s, and final extension at 72° C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…The supernatants were stored at -80° C until they were subjected to liquid chromatography-mass spectrometry (LC-MS). The LC-MS was performed in a Thermo Scientific UltiMate 3000 HPLC system (Thermo Fisher Scientific, Waltham, MA, USA) coupled to a high-resolution tandem Q-Exactive MS (Thermo Fisher Scientific, Waltham, MA, USA) operated in positive and negative ion modes [ 26 ]. An online Kyoto Encyclopedia of Genes and Genomes (KEGG) database ( https://www.genome.jp/kegg/ ) annotated the metabolites by matching their exact molecular mass data, names, and formulae with those in the database.…”

Section: Methodsmentioning

confidence: 99%

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Gut microbial community and fecal metabolomic signatures in different types of osteoporosis animal models

Qiao,

Li,

Wang

et al. 2024

Aging

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Background: The gut microbiota (GM) constitutes a critical factor in the maintenance of physiological homeostasis. Numerous studies have empirically demonstrated that the GM is closely associated with the onset and progression of osteoporosis (OP). Nevertheless, the characteristics of the GM and its metabolites related to different forms of OP are poorly understood. In the present study, we examined the changes in the GM and its metabolites associated with various types of OP as well as the correlations among them. Methods: We simultaneously established rat postmenopausal, disuse-induced, and glucocorticoid-induced OP models. We used micro-CT and histological analyses to observe bone microstructure, three-point bending tests to measure bone strength, and enzyme-linked immunosorbent assay (ELISA) to evaluate the biochemical markers of bone turnover in the three rat OP models and the control. We applied 16s rDNA to analyze GM abundance and employed untargeted metabolomics to identify fecal metabolites in all four treatment groups. We implemented multi-omics methods to explore the relationships among OP, the GM, and its metabolites. Results: The 16S rDNA sequencing revealed that both the abundance and alterations of the GM significantly differed among the OP groups. In the postmenopausal OP model, the bacterial genera g__Bacteroidetes_unclassified, g__Firmicutes_unclassified, and g__Eggerthella had changed. In the disuse-induced and glucocorticoid-induced OP models, g__Akkermansia and g__Rothia changed, respectively. Untargeted metabolomics disclosed that the GM-derived metabolites significantly differed among the OP types. However, a Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that it was mainly metabolites implicated in lipid and amino acid metabolism that were altered in all cases. An association analysis indicated that the histidine metabolism intermediate 4-(β-acetylaminoethyl) imidazole was common to all OP forms and was strongly correlated with all bone metabolism-related bacterial genera. Hence, 4-(β-acetylaminoethyl) imidazole might play a vital role in OP onset and progression. Conclusions: The present work revealed the alterations in the GM and its metabolites that are associated with OP. It also disclosed the changes in the GM that are characteristic of each type of OP. Future research should endeavor to determine the causal and regulatory effects of the GM and the metabolites typical of each form of OP.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Gut microbial community and fecal metabolomic signatures in different types of osteoporosis animal models

Qiao,

Li,

Wang

et al. 2024

Aging

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“…For the six experimental groups, both pre-and posttreatment samples were scanned by a micro-CT system (vivaCT80, SCANCO Medical AG, Switzerland) at high resolution with four OCAs per group. 31 OCAs were scanned, and successive micro-CT two-dimensional (2D) images were obtained with an image resolution of 1024 Â 1024 with a pixel size of 20 Â 20 μm, and a layer spacing of 15.6 μm. The OCA was rinsed with sterile saline for 2 min and rotated every 10 s. Per plug, 1 L saline was used Ultrasound (n = 21)…”

Section: Micro-computed Tomography (Micro-ct) Evaluationmentioning

confidence: 99%

High‐Speed Centrifugation Efficiently Removes Immunogenic Elements in Osteochondral Allografts

Ma,

Lin,

Yang

et al. 2024

Orthopaedic Surgery

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ObjectivesThe current clinical pulse lavage technique for flushing fresh osteochondral allografts (OCAs) to remove immunogenic elements from the subchondral bone is ineffective. This study aimed to identify the optimal method for removing immunogenic elements from OCAs.MethodsWe examined five methods for the physical removal of immunogenic elements from OCAs from the femoral condyle of porcine knees. We distributed the OCAs randomly into the following seven groups: (1) control, (2) saline, (3) ultrasound, (4) vortex vibration (VV), (5) low‐pulse lavage (LPL), (6) high‐pulse lavage (HPL), and (7) high‐speed centrifugation (HSC). OCAs were evaluated using weight measurement, micro‐computed tomography (micro‐CT), macroscopic and histological evaluation, DNA quantification, and chondrocyte activity testing. Additionally, the subchondral bone was zoned to assess the bone marrow and nucleated cell contents. One‐way ANOVA and paired two‐tailed Student's t‐test are used for statistical analysis.ResultsHistological evaluation and DNA quantification showed no significant reduction in marrow elements compared to the control group after the OCAs were treated with saline, ultrasound, or VV treatments; however, there was a significant reduction in marrow elements after LPL, HPL, and HSC treatments. Furthermore, HSC more effectively reduced the marrow elements of OCAs in the middle and deep zones compared with LPL (p < 0.0001) and HPL (p < 0.0001). Macroscopic evaluation revealed a significant reduction in blood, lipid, and marrow elements in the subchondral bone after HSC. Micro‐CT, histological analyses, and chondrocyte viability results showed that HSC did not damage the subchondral bone and cartilage; however, LPL and HPL may damage the subchondral bone.ConclusionHSC may play an important role in decreasing immunogenicity and therefore potentially increasing the success of OCA transplantation.

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“…IPA derived from tryptophan metabolism by intestinal microbiota, promotes osteoblast differentiation by enhancing mitochondrial transcriptional activator Tfam (39). Moreover, OP has been associated with increased bile acid secretion, with bile acids affecting GM composition and altering bile acid metabolism, ultimately impacting osteoblast activity and bone mass (40).…”

Section: Analysis Of Abstractmentioning

confidence: 99%

“…Cluster 5 revolves around the "intestinal mucosal barrier" in OP. The integrity of the intestinal mucosal barrier, acting as a protective interface between the body and the external environment, is crucial in preventing the entry of harmful substances, including toxins and bacteria, into the body (40). GM is pivotal in in uencing bone metabolism by affecting mucosal barrier function.…”

Section: Analysis Of Abstractmentioning

confidence: 99%

Association between gut microbiota and osteoporosis from 2012 to 2023: Bibliometric analysis and global trends

Hou,

Xiao,

Tian

et al. 2023

Preprint

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Background Osteoporosis (OP) is a chronic systemic metabolic bone disease. As the population ages, the prevalence of OP is gradually increasing, making it one of the significant public health problems worldwide. The gut microbiota (GM) is a complex microbial ecosystem that can affect bone metabolism through multiple pathways. Therefore, understanding the potential pathogenesis of the GM is crucial for preventing and treating OP. Methods We conducted a literature search encompassing English-language studies on the relationship between GM and OP published from 2012 to 2023 in the Web of Science Core Collection (WoSCC) database. We employed bibliometric analysis tools, including VOSviewer, CiteSpace, Scimago Graphica, and R Studio, to analyze global trends and research hotspots. Results We analyzed 647 eligible publications, comprising 426 articles and 221 reviews. Over the past 11 years, there has been a consistent exponential growth in the number of articles addressing GM and OP. The top three contributing countries (China, USA, and Japan) collectively accounted for 75.53% of all published articles. Chinese scholars have demonstrated remarkable academic productivity in this field, while American scholars have exhibited substantial academic impact. Furthermore, the University of Gothenburg emerged as the institution with the most significant number of papers related to the GM and OP relationship (n = 16), and the University of Pennsylvania boasted the highest average citation rate (value = 64.56). Among authors, Narayanan Parameswaran and Laura R. McCabe from the USA stood out for having the highest number of publications and the highest average citation value, respectively. Future research directions can be classified into five primary categories: "GM and OP," "immune regulation," "endocrine signaling factors," "GM metabolites," "brain-gut-bone," and "intestinal mucosal barrier." Conclusion Recent years have witnessed substantial progress in research on the role of the GM in OP. A comprehensive review of the published literature underscores the pivotal role of the GM in the pathogenesis and treatment of OP.

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Gut microbiota and fecal metabolic signatures in rat models of disuse-induced osteoporosis

Cited by 8 publications

References 60 publications

Gut microbial community and fecal metabolomic signatures in different types of osteoporosis animal models

Gut microbial community and fecal metabolomic signatures in different types of osteoporosis animal models

High‐Speed Centrifugation Efficiently Removes Immunogenic Elements in Osteochondral Allografts

Association between gut microbiota and osteoporosis from 2012 to 2023: Bibliometric analysis and global trends

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