A modified rabbit model of tracheal stenosis and a household endoscope. More simplicity and accessibility

Zhang, Guoying; Wang, Jianming; Zeng, Yiming

doi:10.1590/acb351104

Cited by 9 publications

(13 citation statements)

References 18 publications

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“…Thirty-five male New Zealand white rabbits (SLAC Laboratory Animal Co., Ltd, Shanghai, China), weighing 4.0–5.0 kg (8 months of age), were used to create New Zealand rabbit models of tracheal stenosis using the modified method we previously established [ 32 ]. In the granulation hyperplasia stage (1 week after modeling), modeling rabbits were randomly divided into the NC group and β-elemene group (8 per group).…”

Section: Methodsmentioning

confidence: 99%

“…The methods of endoscopic measurement of stenosis rate, stenotic tracheal segment sampling, fixation, paraffin embedding and H&E staining were the same as those described in our prior work [ 32 ]. Primary antibodies used for Western blotting of the tissue protein were: Mouse anti-ILK (1:500, Santa Cruz Biotechnology, Cat# sc-20019 RRID: AB_627807), Mouse anti-Akt (1:1000, Cell Signaling Technology, Cat#2920 RRID: AB_1147620), Mouse anti-Phospho-Akt (Ser473) (1:1000, Cell Signaling Technology, Cat# 4051 RRID: AB_331158), Mouse anti-cyclin D1 (1:1000, Cell Signaling Technology, Cat# 2906 RRID: AB_2070400), Mouse anti-Bcl-2 (1:1000, Cell Signaling Technology, Cat#15071 RRID: AB_2744528).…”

Section: Methodsmentioning

confidence: 99%

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β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275

2021

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Background We have previously found that β-elemene could inhibit the viability of airway granulation fibroblasts and prevent airway hyperplastic stenosis. This study aimed to elucidate the underlying mechanism and protective efficacy of β-elemene in vitro and in vivo. Methods Microarray and bioinformatic analysis were used to identify altered pathways related to cell viability in a β-elemene-treated primary cell model and to construct a β-elemene-altered ceRNA network modulating the target pathway. Loss of function and gain of function approaches were performed to examine the role of the ceRNA axis in β-elemene's regulation of the target pathway and cell viability. Additionally, in a β-elemene-treated rabbit model of airway stenosis, endoscopic and histological examinations were used to evaluate its therapeutic efficacy and further verify its mechanism of action. Results The hyperactive ILK/Akt pathway and dysregulated LncRNA-MIR143HG, which acted as a miR-1275 ceRNA to modulate ILK expression, were suppressed in β-elemene-treated airway granulation fibroblasts; β-elemene suppressed the ILK/Akt pathway via the MIR143HG/miR-1275/ILK axis. Additionally, the cell cycle and apoptotic phenotypes of granulation fibroblasts were altered, consistent with ILK/Akt pathway activity. In vivo application of β-elemene attenuated airway granulation hyperplasia and alleviated scar stricture, and histological detections suggested that β-elemene's effects on the MIR143HG/miR-1275/ILK axis and ILK/Akt pathway were in line with in vitro findings. Conclusions MIR143HG and ILK may act as ceRNA to sponge miR-1275. The MIR143HG/miR-1275/ILK axis mediates β-elemene-induced cell cycle arrest and apoptosis of airway granulation fibroblasts by modulating the ILK/Akt pathway, thereby inhibiting airway granulation proliferation and ultimately alleviating airway stenosis.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275

2021

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“…Once anesthetized, the rabbits were placed and fixed in a supine position. Rabbit models of airway stenosis were prepared as according to previously reported methods (Nakagishi et al, 2005;Zhang et al, 2020). After making a midline skin incision in the anterior neck, the larynx and trachea were exposed.…”

Section: Experimental Set-upmentioning

confidence: 99%

Evaluation of Tracheal Stenosis in Rabbits Using Multispectral Optoacoustic Tomography

Wen

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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Objective: Photoacoustic tomography (PAT) and multispectral optoacoustic tomography (MSOT) are evolving technologies that are capable of delivering real-time, high-resolution images of tissues. The purpose of this study was to evaluate the feasibility of using PAT and MSOT for detecting histology in a rabbit tracheal stenosis model. Method: A total of 12 rabbits (9 stenosis and three control) were randomly divided into four groups (A, B, C and D). Each group consisted of three rabbits, which were staged at the first, fourth, and eighth weeks of stenosis progression, respectively. PAT/MSOT images and corresponding histology from these experimental animals were compared, for analyzing the morphologic features and quantitative tracheal measurements in different tracheal stenosis stage.Result: Both the PAT images and corresponding histology indicated the most severe degree of stenosis in group C. MSOT images indicated notable differences in tracheal contents of group B and D.Conclusion: This study suggests that PAT/MSOT are potentially valuable non-invasive modality which are capable of evaluating tracheal structure and function in vivo.

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“…Clinical translation often demands for Proof-of-concept validation in animal models, hence performing in vivo experiments is the way forward to validate the use of the intubation catheter. To study the health and disease condition of the airway, research groups have performed experiments on canines with induced tracheal stenosis [31], porcine tracheal tissues [32], rabbits [33] and sheep [34]. We envisage performing in vivo experiments using sheep models because their comparable airway anatomical structure with humans and their large tracheal diameters makes it most favorable for respiratory intervention studies [35].…”

Section: Blind Test For Pulsatile Flowmentioning

confidence: 99%

An intubation catheter integrated with flow sensors and smart actuators for characterizing airflow patterns in stenosed trachea: an objective guide for CAO management

Alekya¹,

Sitaramgupta²,

Arjun³

et al. 2021

J. Micromech. Microeng.

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Stenosis reduces the effective lumen area in the tracheal and bronchial segments of the airway anatomy. Loss in patency due to obstruction increases resistance to airflow; thus, severe narrowing is often associated with morbidity and mortality. Etiologies such as congenital tracheal stenosis, tracheomalacia, laryngeal and subglottic stenosis, atresia are few among the many pathologies causing major airway obstruction and respiratory distress. Diagnosis of such anomalies is usually based on clinical suspicion due to the non-specificity of the associated clinical symptoms. Visual assessment using conventional bronchoscopy or radiography images from CT scan for precisely locating obstruction site is highly subject to clinician's expertise. Characterizing airflow patterns in stenosed airway calls for newer diagnostic tools that can effectively quantify changes in airflow due to construction sites. Our work presents a steerable intubation catheter that can quantitatively measure air velocity across various segments of the tracheobronchial tree. The catheter consists of a three-layer flexible printed circuit board integrated with micro-electro-mechanical system-based thermal flow sensors and a pair of sub-millimeter helical shape memory actuators. Flow distribution is measured in excised sheep tracheal tissues at 15, 30, 50, 65, and 80 l min −1 for normal and stenosed conditions. Even a 10% reduction in lumen area generated unique peaks corresponding to the obstruction site; thus, the catheter can locate stenosis at the precritical stage. For 50% tracheal obliteration, the sensor closest to stenosis showed a 2.4-fold increase in velocity when tested for reciprocating flows. Thus, flow rate scales quadratically with reducing cross-section area, contributing to increased airflow resistance.

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A modified rabbit model of tracheal stenosis and a household endoscope. More simplicity and accessibility

Cited by 9 publications

References 18 publications

β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275

β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275

Evaluation of Tracheal Stenosis in Rabbits Using Multispectral Optoacoustic Tomography

An intubation catheter integrated with flow sensors and smart actuators for characterizing airflow patterns in stenosed trachea: an objective guide for CAO management

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