Evaluation of Tracheal Stenosis in Rabbits Using Multispectral Optoacoustic Tomography

Abstract

Objective: Photoacoustic tomography (PAT) and multispectral optoacoustic tomography (MSOT) are evolving technologies that are capable of delivering real-time, high-resolution images of tissues. The purpose of this study was to evaluate the feasibility of using PAT and MSOT for detecting histology in a rabbit tracheal stenosis model. Method: A total of 12 rabbits (9 stenosis and three control) were randomly divided into four groups (A, B, C and D). Each group consisted of three rabbits, which were staged at the first, fourth, and eighth weeks of stenosis progression, respectively. PAT/MSOT images and corresponding histology from these experimental animals were compared, for analyzing the morphologic features and quantitative tracheal measurements in different tracheal stenosis stage.Result: Both the PAT images and corresponding histology indicated the most severe degree of stenosis in group C. MSOT images indicated notable differences in tracheal contents of group B and D.Conclusion: This study suggests that PAT/MSOT are potentially valuable non-invasive modality which are capable of evaluating tracheal structure and function in vivo.

Clinical photoacoustic/ultrasound dual-modal imaging: Current status and future trends

“…Over the recent years, the number of studies on PAT systems in the literature has been increasing rapidly ( Su et al, 2010 ; Beard, 2011 ; Menke, 2015 ; Wang et al, 2016 ; Upputuri and Pramanik, 2017 ; Dong et al, 2017 ; Choi et al, 2018 ; Jo et al, 2018 ; Li X et al, 2018 ; Lin et al, 2018 ; Manohar and Dantuma, 2019 ; Nyayapathi and Xia, 2019 ; Steinberg et al, 2019 ; Zhao et al, 2019 ; Duan et al, 2020 ; Manohar and Gambhiret, 2020 ; Deán-Ben and Razanskyet, 2021 ; Gröhl et al, 2021 ; Li et al, 2021 ; Wen Y. T. et al, 2022 ; Yang and Ghim, 2021 ; Wen YT. et al, 2022 ).…”