Inhibition of browning problem during the callogenesis of Spartium junceum L.

Taghizadeh, Mina; Dastjerdi, Mahboubeh Ganji

doi:10.1590/2447-536x.v27i1.2230

Cited by 10 publications

(6 citation statements)

References 26 publications

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“…Some studies have shown that the application of PVP in a culture medium cause elimination of phenolic compounds and reduces the browning of explants 67 , 68 . Because PVP can adsorb polyphenols, it can be argued that PVP, as a polyphenol absorbing agent, is effective in reducing vascular occlusion and has positive synergistic effects with MWCNTs in improving water uptake by the stem.…”

Section: Discussionmentioning

confidence: 99%

Multi-walled carbon nanotubes wrapped with polyvinylpyrrolidone can control the leaf yellowing of Alstroemeria cut flowers

Mousavi

Roein

Hekmatara

2022

Sci Rep

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The rapid yellowing of the leaves on cut flowers with leafy stems severely limits their vase life and commercial value. In this study, the effect of a composite of multi-walled carbon nanotubes (MWCNTs) and polyvinyl pyrrolidone (PVP) on the longevity of cut Alstroemeria flowers (Alstroemeria hybrida) was investigated to obtain a solution to this problem. A range of MWCNTs/PVP composite concentrations (0, 3, 6, and 9 mg L−1) was applied in a vase solution (for 24 h) as pulse treatments. Our findings indicate that the composite of MWCNTs and PVP exhibits excellent dispersibility in a vase solution. The results demonstrate that a 3 mg L−1 MWCNTs/PVP concentration was the most effective, extending the vase life of cut Alstroemeria flowers by up to 27 days. Pulsing with MWCNTs/PVP delayed the onset of floret abscission and leaf yellowing by 5 and 18 days, respectively. Additionally, when MWCNTs/PVP solution was applied to cut stems, water uptake remained consistently greater than that of the control. Additionally, MWCNTs/PVP increased the total chlorophyll content, soluble protein content, and POX enzyme activity of leaves while decreasing the malondialdehyde (MDA) content. The results indicate that this composite exhibited antimicrobial activity against gram-positive and -negative bacteria, particularly at a concentration of 3 mg L−1. This study demonstrated that adding MWCNTs/PVP to a vase solution of Alstroemeria cut flowers increased their longevity with minimal leaf yellowing symptoms compared to untreated cut stems. As a result, this nanocomposite can be used safely and effectively in vase solutions and in combination with other preservatives.

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Section: Discussionmentioning

confidence: 99%

Multi-walled carbon nanotubes wrapped with polyvinylpyrrolidone can control the leaf yellowing of Alstroemeria cut flowers

Mousavi

Roein

Hekmatara

2022

Sci Rep

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“…Ndakidemi et al (2014) reported that incorporation of 200-250 mg/litre of ascorbic acid into the medium significantly controlled lethal browning in nodal culture of Brahylaena huillensis. Supplementation of 0.5% PVP into culture medium prevented explants browning in callus culture of nodal explant of Spartium junceum L. (Taghizadeh and Dastjerd, 2021).…”

Section: Incorporation Of Antioxidants Into Mediummentioning

confidence: 94%

Control of browning in plant tissue culture: A review

Amente

Chimdessa

2021

J Sci Agri

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Browning is one of the severe problems in plant tissue culture that hampers successful in vitro propagation of plants especially woody and perennial plants. In order to control the browning problem, different efforts has been made in vitro such as presoaking of explants in antioxidant solution, incorporation of antioxidants into medium, culturing in the dark period and frequent subculturing of explants. Presoaking of explants in antioxidant solutions like polyvinylpyrolidone (PvP) and ascorbic acid (AC) is one of the most frequently used. Incorporation of antioxidants such as 0.2-0.5g/l PvP and 15-250mg/l ascorbic acid into MS medium is commonly used to control browning in different plants and explants followed by activated charcoal, citric acid, MES and AIP. Moreover, frequent sub culturing and incubation of explants in the dark period is the other alternative. This review article includes the study of previous and current research achievements in a comprehensive way on the different methods to control browning problem in plant tissue culture and suggests further optimization for successful control of browning when using the same or different crops as well explants.

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“…The redness of color could be caused by the long period of culture and the redness color could cause the dead of callus [8]. To overcome this problem, the media need to add PolyvinylPyrrolidone (PVP) in future research [9]. In table 4 above showed that 2,4D0.5 Pic2 media gave callus the best result of callus proliferation of Sumenep variety.…”

Section: Callus Proliferationmentioning

confidence: 99%

Application of combination of auxins in callus induction and proliferation of shallot cv Trisula and Sumenep through in vitro technique

Rahayu,

Purnamaningsih,

Husni

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Shallot (Allium cepa L. var. aggregatum) is one of a strategic commodity in Indonesia. To increase of production of shallot seed, there needs a good tuber with high productivity tolerant to abiotic stress. One of the obstacles to increase of shallot production with tuber in good quality and tolerant to abiotic stress is not available. Tissue culture technique is one of the methods proved can propagate plant in large amounts and in a shorter time compared to the conventional method. The objectives of this research are to get the best media formulation for callus induction and proliferation. The explant used in this research are shoot tip of shallot from cultivar Trisula and Sumenep. The Media used were MS. The medium was enriched with (0,5 – 3 mg/l) 2,4-D + 2 mg/l picloram (0,5 – 3 mg/l) or NAA + with or without 3 gr/l Casein hydrolysate; and MS + 3 mg/l Picloram + 2 or 3 mg/l NAA + 3 g/l Casein Hydrolysate. The best media formulation for callus induction of Trisula variety was MS + 3 mg/l 2,4D + 2 mg/l picloram + 3 g/l Casein Hydrolysate, whereas the best media formulation for callus induction of Sumenep variety was MS + 0.5 mg/l 2,4-D + 2 mg/l picloram. The best media formulation for callusproliferation was MS + 0.5 mg/l 2,4-D + 2 mg/l picloram both Trisula and Sumenep varieties. These results could be used for plant propagation and breeding program of shallot in the future.

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Inhibition of browning problem during the callogenesis of Spartium junceum L.

Cited by 10 publications

References 26 publications

Multi-walled carbon nanotubes wrapped with polyvinylpyrrolidone can control the leaf yellowing of Alstroemeria cut flowers

Multi-walled carbon nanotubes wrapped with polyvinylpyrrolidone can control the leaf yellowing of Alstroemeria cut flowers

Control of browning in plant tissue culture: A review

Application of combination of auxins in callus induction and proliferation of shallot cv Trisula and Sumenep through in vitro technique

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