Detecting alterations of glucose and lipid components in human serum by near-infrared Raman spectroscopy

Borges, Rita de Cássia Fernandes; Navarro, Ricardo Scarparo; Giana, Héctor Enrique; Tavares, Fernanda Grubisich; Fernandes, Adriana Barrinha; Silveira, Landulfo

doi:10.1590/2446-4740.0593

Cited by 25 publications

(6 citation statements)

References 38 publications

(72 reference statements)

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“…More recently, a great emphasis has been given to develop optical novel non-invasive procedure to provide point-of-care sample information using other accessible biological fluids, such as sweat. The results obtained have proven the potential of measuring the glucose concentration of biological body fluids using the optical methods, such as Infra-red spectroscopy, Raman Spectroscopy, with reasonable correlation with blood measurements [4][5][6][7][8][9][10][11][12][13]. Such correlation between the blood & urine measurements has been verified in a preliminary work done by us on 20 subjects with varying levels of insulin intolerance using Laser induced Fluorescence spectroscopy.…”

Section: Introductionsupporting

confidence: 54%

A Portable Laser Urine Glucose Meter – a Preliminary Investigation

Al-Saleh

Masilamani²,

Vignesh³

2022

JPRI

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The most common method of detection/monitoring Diabetic Meletus (DM) is to draw capillary blood and measure pre-prandial or post- prandial glucose level. In addition, HbA1c is often done to measure glycated red blood cell (RBC) to monitor glucose level in blood over a certain period time (about 2 – 3 months). In any case, drawing blood is the only procedure to measure glucose in circulating fluid, blood. It is minimally invasive procedure and with some level of pain and discomfort. Yet, it is indeed an ordeal process of sharp needle pricking the tissue for repeated monitoring, particularly for those who have been diagnosed with Type 1DM and have to maintain strict control, The minor discomfort becomes an issue for old persons, infants and juvenile (of Type 1DM). If there could be a procedure and reliable technique to measure and monitor glucose in urine, the whole population of DM, will heave a sigh of relief. Also, if glucose level in urine, for borderline cases could be identified, this will be a major step for mass screening, more importantly for poor villagers of many Asian and African countries. The purpose of this research is to device a portable laser spectrometer for monitoring glucose concentration in urine. It is a preliminary study, done as a proof of concept, to explore the possibilities of reliable, alternative for DM detection technique.

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Section: Introductionsupporting

confidence: 54%

A Portable Laser Urine Glucose Meter – a Preliminary Investigation

Al-Saleh

Masilamani²,

Vignesh³

2022

JPRI

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“…The line at 1614 cm −1 corresponds to the intermolecular β-sheets [ 25 ], and its contribution in the averaged spectra is about 6%. The intermolecular β-sheets are characterized by stronger hydrogen bonds [ 16 ]. The bands originating from the amino acid side chains vibrations are also observed (1607 cm −1 ) [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia

et al. 2021

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Early detection of the most common pediatric neoplasm, B-cell precursor lymphoblastic leukemia (BCP-ALL), is challenging and requires invasive bone marrow biopsies. The purpose of this study was to establish new biomarkers for early screening to detect pediatric leukemia. In this small cohort study, Fourier transform infrared (FTIR) spectra were obtained from blood sera of 10 patients with BCP-ALL and were compared with the control samples from 10 children with some conditions other than neoplasm. Using various analytical approaches, including a new physical model, some significant differences were observable. The most important include: the different peak area ratio 2965/1645 cm−1 (p = 0.002); the lower average percentage of both β-sheet and β-turn protein structures in the sera of BCP-ALL patients (p = 0.03); an AdaBoost-based predictive model for classifying healthy vs. BCP-ALL patients with 85% accuracy; and the phase shift of the first derivative in the spectral range 1050–1042 cm−1 correlating with white blood cell (WBC) and blast cell count in BCP-ALL patients contrary to the samples obtained from healthy controls. Although verification in larger groups of patients will be necessary, these promising results suggest that FTIR spectroscopy may have future potential for the early screening of BCP-ALL.

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“…4 Additionally, alterations in lipid metabolism may lead to conditions such as familial hypercholesterolemia, hypertriglyceridemia, and low high-density lipoprotein (HDL). 5 RS is used to analyze human serum samples for various biochemical components such as glucose, cholesterol, lipids, 6 phenylalanine, 7 etc. for the detection of respective metabolic disorders mentioned above.…”

Section: ■ Introductionmentioning

confidence: 99%

Temporal In Vitro Raman Spectroscopy for Monitoring Replication Kinetics of Epstein–Barr Virus Infection in Glial Cells

et al. 2020

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Raman spectroscopy can be used as a tool to study virus entry and pathogen-driven manipulation of the host efficiently. To date, Epstein–Barr virus (EBV) entry and altered biochemistry of the glial cell upon infection are elusive. In this study, we detected biomolecular changes in human glial cells, namely, HMC-3 (microglia) and U-87 MG (astrocytes), at two variable cellular locations (nucleus and periphery) by Raman spectroscopy post-EBV infection at different time points. Two possible phenomena, one attributed to the response of the cell to viral attachment and invasion and the other involved in duplication of the virus followed by egress from the host cell, are investigated. These changes corresponded to unique Raman spectra associated with specific biomolecules in the infected and the uninfected cells. The Raman signals from the nucleus and periphery of the cell also varied, indicating differential biochemistry and signaling processes involved in infection progression at these locations. Molecules such as cholesterol, glucose, hyaluronan, phenylalanine, phosphoinositide, etc. are associated with the alterations in the cellular biochemical homeostasis. These molecules are mainly responsible for cellular processes such as lipid transport, cell proliferation, differentiation, and apoptosis in the cells. Raman signatures of these molecules at distinct time points of infection indicated their periodic involvement, depending on the stage of virus infection. Therefore, it is possible to discern the details of variability in EBV infection progression in glial cells at the biomolecular level using time-dependent in vitro Raman scattering.

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Detecting alterations of glucose and lipid components in human serum by near-infrared Raman spectroscopy

Cited by 25 publications

References 38 publications

A Portable Laser Urine Glucose Meter – a Preliminary Investigation

A Portable Laser Urine Glucose Meter – a Preliminary Investigation

A Preliminary Study of FTIR Spectroscopy as a Potential Non-Invasive Screening Tool for Pediatric Precursor B Lymphoblastic Leukemia

Temporal In Vitro Raman Spectroscopy for Monitoring Replication Kinetics of Epstein–Barr Virus Infection in Glial Cells

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