IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals

Rodrigues, Kathryna Fontana; Pietrani, Nathalia Teixeira; Bosco, Adriana; Campos, Fernanda Magalhães Freire; Sandrim, Valéria C.; Gomes, Karina Braga

doi:10.1590/2359-3997000000254

Cited by 95 publications

(69 citation statements)

References 51 publications

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“…In the present study, levels of proinflammatory cytokines such as TNF‐alpha, IL‐6 and anti‐inflammatory cytokine IL‐10 were significantly higher in T2DM individuals compared to controls. Similar results have been reported in T2DM patients from other countries 17‐21 . However, some study reported that there was no significant difference in TNF‐alpha, 22 IL‐6, 23 and IL‐10 24 between T2DM and controls.…”

Section: Discussionsupporting

confidence: 84%

Oxidative stress and inflammatory markers in type 2 diabetic patients

Malik

Morya

Saha

et al. 2020

Eur J Clin Investigation

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Background: Type 2 diabetes mellitus (T2DM) is most demanding public health problem of 21st century. Uncontrolled diabetes may cause complications affecting any part of gut from mouth to rectum presenting as vomiting, nausea, bloating, abdominal pain, constipation and diarrhoea. The aim of this study was to compare levels of oxidative stress and inflammatory markers in small intestinal bacterial overgrowth (SIBO)-positive and negative diabetic patients. Subjects and methods: An observational analytical study was conducted on 300 T2DM (>5 years' duration) attending Diabetic Clinic. A total of 200 age-and sexmatched healthy individuals were enrolled as controls. Noninvasive glucose hydrogen breath test was used to diagnose SIBO. A total of 5 mL blood was taken. Plasma was used for measurement of inflammatory cytokines (TNF-α, IL-6 and IL-10) by ELISA. Hemolysate was used for measurement of lipid peroxidation, reduced GSH, superoxide dismutase and catalase. Results: It was observed that constipation was present in 59.6% T2DM patients.SIBO was observed significantly higher (P < .0001) in T2DM patients than controls.Inflammatory and oxidative stress markers were significantly (P < .001) higher in diabetic and SIBO-positive patients than controls and SIBO negative. Reduced GSH was significantly (P < .05) lower whereas superoxide dismutase (SOD) and catalase antioxidant enzymes were significantly (<.05) higher in diabetic and SIBO-positive patients than controls and SIBO-negative patients. Conclusion: From this study, it could be concluded that SIBO in T2DM patients can cause oxidative stress and inflammation. Therefore, SIBO should be taken care to prevent further damage to intestine. K E Y W O R D Sinflammatory markers, orocecal transit time, oxidative stress, small intestinal bacterial over growth, type 2 diabetes mellitus 2 of 6 | MALIK et AL.

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Section: Discussionsupporting

confidence: 84%

Oxidative stress and inflammatory markers in type 2 diabetic patients

Malik

Morya

Saha

et al. 2020

Eur J Clin Investigation

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“…In a study of 120 Caucasian Italian women no AA homozygotes were recognized and the frequency of A allele was 27.4% [90]. The frequency of AA genotype was about 4% among Han Chinese [91], 2% among people with obesity from Spain [92], and 0.6% in the Brazilian individuals [93]. In GG homozygotes higher plasma levels of TNF-α and CRP-hs than in AA homozygotes [94][95][96][97] as well as in GA heterozygotes of the G-308A polymorphism in the TNF-α gene were reported [90].…”

Section: Discussionmentioning

confidence: 99%

TNF-α G-308A genetic variants, serum CRP-hs concentration and DNA damage in obese women

Włodarczyk

Ciebiera²

2019

Mol Biol Rep

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Obesity is associated with inflammation, which can disturb genome stability. Tumor necrosis factor (TNF-α) polymorphism was found to affect TNF-α protein production and inflammation. Therefore, the present study illustrates the relationship between TNF-α polymorphism, the degree of inflammation assessed by serum high sensitivity C-reactive protein concentration (CRP-hs) and basal DNA damage in patients with obesity (BMI 30-34.9 kg/m 2) and control subjects with proper body mass (BMI < 25 kg/m 2). A total of 115 participants (75 obese premenopausal women; and 40 age-, and gender-matched controls) were included. Biochemical parameters (serum concentrations of total-cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides, glucose, apolipoprotein AI, CRP-hs) and endogenous DNA damage (determined by comet assay) were measured. TNF-α G-308A polymorphism (rs1800629) was analyzed by PCR-RFLP (PCR-restriction fragments length polymorphism). An effect of TNF-α genotype on serum CRP-hs concentration was noted (p = 0.031). In general, carriers of the rare A allele of the TNF-α G-308A polymorphism had significantly lower endogenous DNA damage and serum CRPhs concentrations than GG homozygotes, however, the protective effect of the A allele was especially visible in non-obese women. Serum CRP-hs concentrations and levels of DNA damage (% DNA in tail) were significantly higher in obese than in controls (p = 0.001 and p < 0.0001, respectively). The adjusted multiple linear regression analyses revealed a significant, independent impact of obesity on DNA damage (p = 0.00000) and no effect of other covariates i.e. age, TNF-α genotype and serum CRP-hs concentration. Our study showed that obesity has a significant impact on the levels of endogenous DNA damage. Obesity abolished the protective effect of A allele of the TNF-α G-308A polymorphism on DNA damage and on inflammation development observed in non-obese A allele carriers.

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“…Correspondingly, this study only recorded a mild correlation between these cytokines (R = −0.656). Similarly, other studies have reported their contrasting properties regarding insulin resistance (Daniele et al 2014;Rodrigues et al 2017).…”

Section: Discussionmentioning

confidence: 89%

The synergistic effect of fenretinide and metformin to achieve a decrease in insulin resistance and inflammatory mediators: an in vivo study

et al. 2020

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Background: The effects of metformin and fenretinide on insulin resistance and inflammatory mediators were investigated to treat type 2 diabetes mellitus. Methods: Type 2 diabetes was induced in adult albino Wistar rats via the administration of streptozotocin and nicotinamide. Rats were divided as follows: Group I, control rats administered normal saline solution; Group II, diabetic rats; Group III, diabetic rats treated with metformin; Group IV, diabetic rats administered fenretinide; and Group V, diabetic rats administered both metformin and fenretinide. Results: Fasting blood glucose (FBG) was increased in diabetic rats compared to control rats (p < 0.05). However, both FBG and glycated hemoglobin levels were decreased (p < 0.05) in diabetic rats treated with fenretinide and metformin compared to diabetic untreated rats. A positive relationship was identified between RBP4 level and insulin resistance. Further, diabetic rats treated with fenretinide and metformin had decreased RBP4 levels. Conversely, GLUT4 mRNA was markedly increased (p < 0.05) in diabetic rats administered fenretinide and metformin, thereby improving insulin sensitivity. TNF-α level showed a direct relationship with the insulin resistance. Evidently, metformin and fenretinide decreased plasma IL-6 level and increased IL-10 levels. Conclusion: Metformin and fenretinide exhibit individual and synergistic effects when administered to treat diabetes in a rat model.

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IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals

Cited by 95 publications

References 51 publications

Oxidative stress and inflammatory markers in type 2 diabetic patients

Oxidative stress and inflammatory markers in type 2 diabetic patients

TNF-α G-308A genetic variants, serum CRP-hs concentration and DNA damage in obese women

The synergistic effect of fenretinide and metformin to achieve a decrease in insulin resistance and inflammatory mediators: an in vivo study

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