New primers for amplification of cytochrome c oxidase subunit I barcode region designed for species of Decapoda (Crustacea)

Mantelatto, Fernando L.; Carvalho, Fabrício Lopes; Simões, Sabrina Morilhas; Negri, Mariana; Souza-Carvalho, Edvanda A.; Terossi, Mariana

doi:10.1590/2358-2936e2016030

Cited by 25 publications

(13 citation statements)

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“…Tissue extraction, PCR amplification, product cleanup, and sequencing were conducted following previously described protocols 52 with some modifications, mainly in product cleanup 53 . The primers and melting temperatures used were: mitochondrial 16S ribosomal gene [1472 (5′-AGATAGAAACCAACCTGG-3′) 54 , 16SL2 (5′-TGCCTGTTTATCAAAAACAT-3′) 55 , 46–48 °C]; mitochondrial Cytocrome Oxidase subunit I (COI) [COL6b (5′-ACAAATCATAAAGATATYGG-3′) and COH6 (5′-TADACTTCDGGRTGDCCAAARAAYCA-3′) 56 , COIAL2o (5′-ACGCAACGATGATTATTTTCTAC-3′) and COIAH2m (5′- GACCRAAAAATCARAATAAATGTTG-3´) 57 , 48–50 °C]; nuclear small subunit 18S ribosomal gene [18S-A (5′-AACCTGGTTGATCCTGCCAGT-3′) and 18S-L (5′-CCAACTACGAGCTTTTTAACTG-3′) 58 , 59 °C]; nuclear Histone 3 (H3) gene [H3AF (5′-ATGGCTCGTACCAAGCAGACVGC-3′) and H3AR (5′-ATATCCTTRGGCATRATRGTGAC-3′) 59 , 46–48 °C].…”

Section: Methodsmentioning

confidence: 99%

Global biogeography, cryptic species and systematic issues in the shrimp genus Hippolyte Leach, 1814 (Decapoda: Caridea: Hippolytidae) by multimarker analyses

Terossi

Grave

Mantelatto

2017

Sci Rep

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Hippolyte is a genus of small bodied marine shrimps, with a global distribution. Here, we studied the phylogenetic and biogeographic relationships amongst the species of this genus with two mitochondrial and two nuclear markers, using Bayesian Inference, Maximum Likelihood, genetic divergence, molecular clock and S-DIVA. In addition, the Indo-West Pacific genus Alcyonohippolyte was included. Based on sequences from 57 specimens of 27 species, we recovered a robust biogeographic scenario that shows the Indo-West Pacific as the probable ancestral area of the genus Hippolyte, which emerged in the Paleocene, followed by dispersal in three general directions: (1) South Pacific, (2) eastern Atlantic and Mediterranean Sea and (3) Americas, the latter with a primary colonization in the eastern Pacific followed by a radiation into the western Atlantic. Our analysis reveals that the species of the H. ventricosa group do not constitute a monophyletic group and Alcyonohippolyte does not constitute a reciprocally monophyletic group to Hippolyte, with both genera herein synonimised. The relationships and systematic status of several transisthmian and Atlantic species are clarified.

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Section: Methodsmentioning

confidence: 99%

Global biogeography, cryptic species and systematic issues in the shrimp genus Hippolyte Leach, 1814 (Decapoda: Caridea: Hippolytidae) by multimarker analyses

Terossi

Grave

Mantelatto

2017

Sci Rep

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“…26 , and the Chelating Ion Exchange Resin (Chelex 100) 65 . Two molecular markers from mitochondrial genes, cytochrome c oxidase subunit I (COI) barcoding region 66–68 (N = 91) and 16 S rDNA 67 (N = 15) were used. Additionally, the Palumbi region of COI 25 (N = 9) was used to allow the comparison of our specimens to those used by Gusmão et al .…”

Section: Methodsmentioning

confidence: 99%

A multigene and morphological analysis expands the diversity of the seabod shrimp Xiphopenaeus Smith, 1869 (Decapoda: Penaeidae), with descriptions of two new species

Carvalho-Batista

Terossi

Zara

et al. 2019

Sci Rep

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After being stable for nearly a century, the taxonomic history of the genus Xiphopenaeus has been marked by many changes in the last three decades. The taxonomic status of the Atlantic species has a low resolution, and many species are still undefined and grouped as cryptic species. Here we employed an integrative approach to define the species of Xiphopenaeus and the morphological characters needed to differentiate them. We combined the analyses of two molecular markers (COI and 16 S rDNA), scanning electron microscopy and light microscopy. Based on specimens from 17 localities from the Atlantic and Pacific oceans, we detected five divergent genetic groups, three in the Atlantic (A1, A2, A3) and two in the Pacific (P1, P2). Male secondary sexual characters were able to differentiate four out of the five genetic groups. Group A1 corresponds to X. kroyeri, and A2 and A3 correspond to new species. We redescribed the genus and two new species are described and illustrated: Xiphopenaeus dincao nov. sp. (A2) and Xiphopenaeus baueri nov. sp. (A3). Since the holotype of X. riveti was missing and the specimen analysed from group P2 was a female, the status of the species of Xiphopenaeus from the Pacific remains unresolved.

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“…1991), and universal variants jgLCO1490/jgHCO2198 (Geller et al . 2013), and using the crustacean specific primer pair COI6Lb/COIAH2m (Schubart & Huber, 2006; Mantelatto et al . 2016) to amplify possible gut contents.…”

Section: Methodsmentioning

confidence: 99%

Pelagic larval polyclads that practice macrophagous carnivory

Mendes

2021

Preprint

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We report evidence that hatchling polyclads of several genera feed in the plankton on large prey. These ciliated swimmers, despite apparently lacking means to concentrate food or even detect it at a distance, subdue and consume fast-moving active-swimming plankters such as crustacean larvae and copepods, or molluskan veligers. We describe feeding events in captivity using videomicroscopy, and identify several wild-caught predatory pelagic polyclad larvae to genus or species level by DNA barcoding. Remarkably, one of these types is identified unambiguously with a species previously observed as Müller's larvae, which live as conventional planktotrophs on an inferred diet of small phytoflagellates. Therefore we conclude first that while so-called "direct-developing" polyclad flatworms may hatch with juvenile-like morphology, at least some of these are functionally larvae. Second, that some species of polyclad have at least a triphasic life cycle, with a first larval stage living in the plankton on primary producers followed by a second larval stage living in the plankton by macrophagous carnivory, before presumably settling to the benthos for adult life.

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New primers for amplification of cytochrome c oxidase subunit I barcode region designed for species of Decapoda (Crustacea)

Cited by 25 publications

References 1 publication

Global biogeography, cryptic species and systematic issues in the shrimp genus Hippolyte Leach, 1814 (Decapoda: Caridea: Hippolytidae) by multimarker analyses

Global biogeography, cryptic species and systematic issues in the shrimp genus Hippolyte Leach, 1814 (Decapoda: Caridea: Hippolytidae) by multimarker analyses

A multigene and morphological analysis expands the diversity of the seabod shrimp Xiphopenaeus Smith, 1869 (Decapoda: Penaeidae), with descriptions of two new species

Pelagic larval polyclads that practice macrophagous carnivory

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