Desiccation tolerance and longevity of germinated Sesbania virgata (Cav.) Pers.seeds

Costa, Maria Cecília D.; Faria, José Márcio Rocha; José, Anderson Cleiton; Ligterink, Wilco; Hilhorst, H.W.M.

doi:10.1590/2317-1545v38n1155510

Cited by 9 publications

(3 citation statements)

References 22 publications

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“…The solution for dehiscence test can be chosen for inducing cross-stress resistance [ 31 , 39 – 41 ] especially drought-tolerance, such as H 2 O 2 , polyethylene glycol (PEG) [ 32 ], abscisic acid (ABA) [ 33 , 40 ] according to experimental experience. We found the optimal concentration of H 2 O 2 was 1 mM (Additional file 2 : Table S1) and did Peng [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Dehiscence method: a seed-saving, quick and simple viability assessment in rice

Lin

Wang

et al. 2018

Plant Methods

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BackgroundSeed viability monitoring is very important in ex situ germplasm preservation to detect germplasm deterioration. This requires seed-, time- and labor- saving methods with high precision to assess seed germination as viability. Although the current non-invasive, rapid, sensing methods (NRSs) are time- and labor-saving, they lack the precision and simplicity which are the virtues of traditional germination. Moreover, they consume a considerable amount of seeds to adjust sensed signals to germination percentage, which disregards the seed-saving objective. This becomes particularly severe for rare or endangered species whose seeds are already scarce. Here we propose a new method that is precise, low-invasive, simple, and quick, which involves analyzing the pattern of dehiscence (seed coat rupture), followed by embryonic protrusion.ResultsDehiscence proved simple to identify. After the trial of 20 treatments from 3 rice varieties, we recognized that dehiscence percentage at the 48th hour of germination (D(48)) correlates significantly with germination rate for tested seed lots. In addition, we found that the final germination percentage corresponded to D(48) plus 5. More than 70% of the seeds survived post-dehiscence desiccation for storage. Hydrogen peroxide (1 mM) as the solution for imbibition could further improve the survival. The method also worked quicker than tetrazolium which is honored as a fast, traditional method, in detecting less vigorous but viable seeds.ConclusionWe demonstrated the comprehensive virtues of dehiscence method in assessing rice seed: it is more precise and easier to use than NRSs and is faster and more seed-saving than traditional methods. We anticipate modifications including artificial intelligence to extend our method to increasingly diverse circumstances and species.Electronic supplementary materialThe online version of this article (10.1186/s13007-018-0334-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Dehiscence method: a seed-saving, quick and simple viability assessment in rice

Lin

Wang

et al. 2018

Plant Methods

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“…, using polyethylene glycol [PEG] solution) and/or using plant hormone (abscisic acid [ABA]) before relatively fast-drying is applied. DT has been successfully re-established for seeds of 12 species ( Table 2 ), including those of herbaceous plants ( e.g., Arabidopsis thaliana , Cucumis sativus , Impatiens walleriana , Medicago truncatula, Pisum sativum , Vigna unguiculata , and Xerophyta viscosa ) and woody plants ( e.g., Cedrela fissilis , Caragana korshinskii , Peltophorum dubium , Sesbania virgata , and Tabebuia impetiginosa ) ( Bruggink and van der Toorn, 1995 ; Buitink et al., 2000 ; Leprince et al., 2000 ; Buitink et al., 2003 ; Leprince et al., 2004 ; Faria et al., 2005 ; Avelange-Macherel et al., 2006 ; Boudet et al., 2006 ; Buitink et al., 2006 ; Vieira et al., 2010 ; Maia et al., 2011 ; Terrasson et al., 2013 ; Lyall et al., 2014 ; Maia et al., 2014 ; Masetto et al., 2014 ; Costa et al., 2015a ; Costa et al., 2015b ; Masetto et al., 2016 ; Costa et al., 2016 ; Guimarães et al., 2016 ; Lang et al., 2017 ; Peng et al., 2017 ; Silva et al., 2017 ; Peng et al., 2018 ; Peng et al., 2021 ; Wang et al., 2021 ).…”

Section: Subsections Relevant For the Subjectmentioning

confidence: 99%

Mechanistic insights derived from re-establishment of desiccation tolerance in germinating xerophytic seeds: Caragana korshinskii as an example

Peng

Huang

et al. 2022

Front. Plant Sci.

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Germplasm conservation strongly depends on the desiccation tolerance (DT) of seeds. Xerophytic seeds have strong desiccation resistance, which makes them excellent models to study DT. Although some experimental strategies have been applied previously, most methods are difficult to apply to xerophytic seeds. In this review, we attempted to synthesize current strategies for the study of seed DT and provide an in-depth look at Caragana korshinskii as an example. First, we analyze congenital advantages of xerophytes in the study of seed DT. Second, we summarize several strategies used to study DT and illustrate a suitable strategy for xerophytic species. Then, based on our previous studies work with C. korshinskii, a feasible technical strategy for DT re-establishment is provided and we provide illustrate some special molecular mechanisms seen in xerophytic seeds. Finally, several steps to unveil the DT mechanism of xerophytic seeds are suggested, and three scientific questions that the field should consider are listed. We hope to optimize and utilize this strategy for more xerophytic species to more systematically decipher the physiological and molecular processes of seed DT and provide more candidate genes for molecular breeding.

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“…Germinating orthodox seeds have been used in studies on the loss of desiccation tolerance to understand the mechanisms linked to desiccation tolerance (Reisdorph and Koster, 1999;Daws et al, 2007;Pereira et al, 2014;Maia et al, 2016). Those studies have been diverse, ranging from physiological and structural characterizations (Reisdorph and Koster, 1999;Guimarães et al, 2011;Pereira et al, 2014) to molecular and ecological analysis (Maia et al, 2011;Costa et al, 2016;Guimarães et al, 2016;Marques et al, 2017). Furthermore, desiccation tolerance reestablishment studies have been carried out to reactivate the mechanisms lost during the germination process (Buitink et al, 2003;Faria et al, 2005;Vieira et al, 2010;Maia et al, 2011;Masetto et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Loss of Desiccation Tolerance in Seeds of Tree Species During Germination: Theoretical and Practical Implications

et al. 2018

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Understanding the loss of desiccation tolerance is of great importance in seed technology for its implications in the development of strategies for seed conservation and seedling production. In the present work, the loss of desiccation tolerance was studied in seeds of tree species Bowdichia virgilioides, Libidibia ferrea, Cedrela fissilis, Enterolobium contortisiliquum, Handroanthus impetiginosus, and Piptadenia gonoacantha. The seeds were collected in the region of Lavras, MG, and subjected to desiccation experiments individually for each species. Imbibition curve was created for each species by measuring seed weight at regular intervals under germination conditions. Based on this information, the seeds were allowed to imbibe for a time inferred from the imbibition curve, and then allowed to dry until reaching the initial water content measured before the imbibition begun. Then, the seeds were rehydrated, and those that developed into normal seedlings were considered desiccation-tolerant. The loss of seed desiccation tolerance followed a specific pattern in each studied species, but none of the species was tolerant to desiccation after radicle protrusion. A drastic decrease in desiccation tolerance was observed in P. gonoacantha seeds at the beginning of the germination process. Under the conditions implemented in this study, the loss of desiccation tolerance in all species started at the beginning of the germination process. These results have implications in both theoretical studies (seed priming) and the practice (in seedling production).

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Desiccation tolerance and longevity of germinated Sesbania virgata (Cav.) Pers.seeds

Cited by 9 publications

References 22 publications

Dehiscence method: a seed-saving, quick and simple viability assessment in rice

Dehiscence method: a seed-saving, quick and simple viability assessment in rice

Mechanistic insights derived from re-establishment of desiccation tolerance in germinating xerophytic seeds: Caragana korshinskii as an example

Loss of Desiccation Tolerance in Seeds of Tree Species During Germination: Theoretical and Practical Implications

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