Protocolo para germinação assimbiótica e desenvolvimento inicial de protocormos de orquídeas nativas do Cerrado brasileiro

Soares, Jackeline Schultz; Sorgato, José Carlos; Ribeiro, Luan Marlon

doi:10.1590/2175-7860202071095

Cited by 4 publications

(1 citation statement)

References 16 publications

(18 reference statements)

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“…1). Soares et al (2020) also reported no significant differences among these culture media for Cattleya lundii and C. nobilior but for Brassavola tuberculata ½ MS provided the best germination response. The lowest germination rate in the present study was verified on full-strength MS medium, which significantly inhibited this process.…”

Section: Germination and Protocorm Developmentmentioning

confidence: 91%

Asymbiotic germination, initial development in vitro and acclimatization of Cyrtopodium paludicolum Hoehne, a Brazilian Savanna orchid species

et al. 2022

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Cyrtopodium paludicolum is a terrestrial orchid species, native to Brazil, whose natural propagation is jeopardized by the intensive collection from the wild and is threatened by agricultural expansion in Cerrado areas. In light of that, this investigation aimed at studying the in vitro germination and early development of C. paludicolum as influenced by culture medium, sucrose and growth regulators as well as its micropropagation by using dark-grown stem segments. A protocol for its acclimatization is also detailed. The effects of Murashige & Skoog (MS), Knudson C (KC) and Vacin & Went (VW) media on the in vitro germination and initial development were tested. The influence of different concentrations of BA, NAA, and of sucrose on plant multiplication and growth were evaluated. The possibility of using etiolated stem segments for micropropagation was also assessed. Acclimatization was accomplished in two phases by using three different substrates. The results showed that VW was the best medium for germination whereas for seedling formation KC was the most advantageous since they were healthy and vigorous. Sucrose at 2% favored the greatest seedling growth and development. Shoot and root proliferation and development were best promoted in the presence of 2.28/2.28 and 0.57/0.57 µM BA/NAA, respectively. The use of etiolated stem segments for micropropagation was effective. Successful acclimatization was accomplished by initially growing plants in community pots containing a 3:1 (v/v) mix of Bioplant and dried Sphagnum moss followed by their transfer to individual pots containing a 2:1 (v/v) mix of Bioplant and Ouro Negro substrates.

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Section: Germination and Protocorm Developmentmentioning

confidence: 91%

Asymbiotic germination, initial development in vitro and acclimatization of Cyrtopodium paludicolum Hoehne, a Brazilian Savanna orchid species

et al. 2022

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Micropropagation of Brassavola nodosa (L.) Lindl. using SETIS™ bioreactor

Vendrame

Beleski

2023

Plant Cell Tiss Organ Cult

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Brassavola nodosa (L.) Lindl. is a tropical epiphytic orchid showing characteristics of interest for the ornamental nursery industry. However, problems with traditional propagation methods limit the development of a large-scale commercial production system. In addition, this species is considered endangered due to the reduction in population caused by habitat destruction, climate change and overcollection from native areas. The use of micropropagation has been investigated for this species, and the use of liquid in vitro systems showed potential for use of temporary immersion bioreactors for micropropagation of B. nodosa. This study evaluated the e ciency of the SETIS™ bioreactor system for the micropropagation of B. nodosa by adjusting parameters of immersion (frequency and duration) and by comparing it to conventional semi-solid culture systems. Results indicate that temporary immersion of B. nodosa explants with a frequency of 2 h and duration of 2 min returned the highest multiplication rates, with 4.6 shoots produced per explant compared with 2.8 shoots per explant in semi-solid agarbased systems. The use of bioreactors also promoted increased growth and development and in vitro rooting, therefore improving survival and facilitating acclimatization of in vitro-derived plantlets. This is the rst study demonstrating a successful protocol for large-scale micropropagation of B. nodosa using SETIS™ bioreactors, which could have signi cant value and impact for the commercial production of this species as well as for conservation purposes. Key MessageMicropropagation of Brassavola nodosa was achieved using SETIS temporary immersion bioreactors, showing good multiplication rates, increased growth and development and in vitro rooting, thus improving survival and acclimatization ex vitro.

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Effects of culture methods and plant growth regulators on in vitro propagation of Brassavola nodosa (L.) Lindl. hybrid

Beleski

Vendrame

2022

In Vitro Cell.Dev.Biol.-Plant

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Brassavola nodosa (L.) Lindl. is an epiphytic orchid with great potential for the ornamental plant industry. The lack of information on propagation and production techniques limits the development of large-scale commercial production. Furthermore, this species is experiencing a reduction in population due to habitat destruction, the impact of climate change, and over-collection from native habitats. This study aimed at developing an efficient protocol for micropropagation of B. nodosa, which could be valuable towards the large-scale commercial production as well as for conservation of this species. Six different concentrations of plant growth regulators (BA or IBA, with or without adenine sulfate) were evaluated in modified Murashige and Skoog (MS) medium for shoot multiplication. In addition, two concentrations of either NAA or IBA were evaluated for rooting. Explants were cultured under three different culture media conditions: semi-solid medium, liquid medium (partial immersion), and liquid medium (complete immersion). Results indicate that B. nodosa could be successfully micropropagated in liquid culture with partial immersion. The modified MS medium supplemented with 2.0 mg L−1 BA and 30.0 mg L−1 adenine sulfate resulted in higher multiplication rates. Rooting was obtained using either 0.5 mg L−1 NAA or 1.0 mg L−1 IBA with no significant differences between both rooting treatments. Plantlets achieved 100% ex vitro survival after 30-d acclimatization.

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Protocolo para germinação assimbiótica e desenvolvimento inicial de protocormos de orquídeas nativas do Cerrado brasileiro

Cited by 4 publications

References 16 publications

Asymbiotic germination, initial development in vitro and acclimatization of Cyrtopodium paludicolum Hoehne, a Brazilian Savanna orchid species

Asymbiotic germination, initial development in vitro and acclimatization of Cyrtopodium paludicolum Hoehne, a Brazilian Savanna orchid species

Micropropagation of Brassavola nodosa (L.) Lindl. using SETIS™ bioreactor

Effects of culture methods and plant growth regulators on in vitro propagation of Brassavola nodosa (L.) Lindl. hybrid

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