Assessment of resistance in common bean to Fusarium oxysporum f. sp. phaseoli using different inoculation and evaluation methods

Paulino, Jean Fausto de Carvalho; Almeida, Caléo Panhoca de; Gonçalves, Gabriel de Moraes Cunha; Bueno, César Júnior; Carbonell, Sérgio Augusto Morais; Chiorato, Alisson Fernando; Bechimol-Reis, Luciana Lasry

doi:10.1590/1984-70332020v20n3n45

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…Likewise, some accessions previously characterized as tolerant to water deficit had good performance in resistance to both strains of Fop , especially the genotypes BAT-477 [ 73 ], SEA-5 [ 74 ], SER-16, and IAC Imperador [ 75 ], with average scores of 1.33, 1.33, 2.65, and 2.0, respectively. BRS Estilo and A211 showed susceptibility to UFV01 through the root-dip inoculation method, with susceptibility levels like those previously reported [ 19 , 55 ]. This may be explained by the origin of UFV01, which was collected in Coimbra (MG, Brazil) and previously characterized as a highly virulent strain [ 20 , 55 ].…”

Section: Resultssupporting

confidence: 82%

“…Among the genotypes, IAC Milênio was used as a Fop -resistant check cultivar and BRS Estilo as a susceptible check cultivar [ 19 ]. Two strains of Fop were used.…”

Section: Methodsmentioning

confidence: 99%

“…Ten days after sowing, the roots of each genotype were washed using distilled water and a third of the length was cut using a sterile scissor (the root-dip method, Paulino et al [ 19 ]. The roots were immediately immersed in a Falcon ® tube containing ten mL of the spore suspension for five minutes.…”

Section: Methodsmentioning

confidence: 99%

“…The development of resistant cultivars is a promising alternative for control of this disease as resistant cultivars are easily adopted by producers and do not cause environmental risks [ 17 , 18 ]. Pathogenicity testing through inoculation methods can be used to characterize the degree of pathogenicity of Fop strains [ 19 ]. Pathogenicity testing also provides an alternative for assessing the diversity of physiological races of the pathogen, the main cause of breakdown in genetic resistance to Fop in bean cultivars [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean

Paulino

Almeida

Bueno

et al. 2021

Genes

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Fusarium wilt (Fusarium oxysporum f. sp. phaseoli, Fop) is one of the main fungal soil diseases in common bean. The aim of the present study was to identify genomic regions associated with Fop resistance through genome-wide association studies (GWAS) in a Mesoamerican Diversity Panel (MDP) and to identify potential common bean sources of Fop’s resistance. The MDP was genotyped with BARCBean6K_3BeadChip and evaluated for Fop resistance with two different monosporic strains using the root-dip method. Disease severity rating (DSR) and the area under the disease progress curve (AUDPC), at 21 days after inoculation (DAI), were used for GWAS performed with FarmCPU model. The p-value of each SNP was determined by resampling method and Bonferroni test. For UFV01 strain, two significant single nucleotide polymorphisms (SNPs) were mapped on the Pv05 and Pv11 for AUDPC, and the same SNP (ss715648096) on Pv11 was associated with AUDPC and DSR. Another SNP, mapped on Pv03, showed significance for DSR. Regarding IAC18001 strain, significant SNPs on Pv03, Pv04, Pv05, Pv07 and on Pv01, Pv05, and Pv10 were observed. Putative candidate genes related to nucleotide-binding sites and carboxy-terminal leucine-rich repeats were identified. The markers may be important future tools for genomic selection to Fop disease resistance in beans.

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Section: Resultssupporting

confidence: 82%

“…Among the genotypes, IAC Milênio was used as a Fop -resistant check cultivar and BRS Estilo as a susceptible check cultivar [ 19 ]. Two strains of Fop were used.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean

Paulino

Almeida

Bueno

et al. 2021

Genes

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“…At present, Fusarium wilt resistance is primarily identified in plants during the seedling stage, with root-dipping being the most commonly employed strategy given that it most closely mimics the route whereby plants are exposed to this pathogen in nature, with this approach having been implemented in cotton [ 32 ], watermelons [ 33 ], bananas [ 34 ], and beans [ 35 ]. This same approach was thus employed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Resource Screening and Inheritance Analysis of Fusarium oxysporum sp. conglutinans Race 2 Resistance in Cabbage (Brassica oleracea var. capitata)

Tong

Zhao

Liu

et al. 2022

Genes

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Cabbage (Brassica oleracea var. capitata) Fusarium wilt (CFW) is a disease that poses a critical threat to global cabbage production. Screening for resistant resources in order to support the breeding of resistant cultivars is the most reliable approach to control this disease. CFW is caused by Fusarium oxysporum f. sp. conglutinans (Foc), which consists of two physiological races (race 1 and 2). While many studies have focused on resistance screening, gene mining, and inheritance-based research associated with resistance to Foc race 1, there have been few studies specifically analyzing resistance to Foc race 2, which is a potential threat that can overcome type A resistance. Here, 166 cabbage resources collected from around the world were evaluated for the resistance to both Foc races, with 46.99% and 38.55% of these cabbage lines being resistant to Foc race 1 and race 2, respectively, whereas 33.74% and 48.80% were susceptible to these two respective races. Of these 166 analyzed cabbage lines, 114 (68.67%) were found to be more susceptible to race 2 than to race 1, and 28 of them were resistant to race 1 while susceptible to race 2, underscoring the highly aggressive nature of Foc race 2. To analyze the inheritance of Foc race 2 resistance, segregated populations derived from the resistant parental line ‘Badger Inbred 16’ and the susceptible one ‘01-20’ were analyzed with a major gene plus polygene mixed genetic model. The results of this analysis revealed Foc race 2-specific resistance to be under the control of two pairs of additive-dominant-epistatic major genes plus multiple additive-dominant-epistatic genes (model E). The heritability of these major genes in the BC1P1, BC1P2, and F2 generations were 32.14%, 72.80%, and 70.64%, respectively. In summary, these results may aid in future gene mining and breeding of novel CFW-resistant cabbage cultivars.

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Molecular and pathogenicity characterization of Fusarium oxysporum species complex associated with Fusarium wilt of common bean in Brazil

Paulino

Almeida

Barbosa

et al. 2022

Trop. plant pathol.

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Assessment of resistance in common bean to Fusarium oxysporum f. sp. phaseoli using different inoculation and evaluation methods

Abstract: Assessment of resistance in common bean to Fusarium oxysporum f. sp. phaseoli using different inoculation and evaluation methods

Cited by 6 publications

References 23 publications

Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean

Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean

Resource Screening and Inheritance Analysis of Fusarium oxysporum sp. conglutinans Race 2 Resistance in Cabbage (Brassica oleracea var. capitata)

Molecular and pathogenicity characterization of Fusarium oxysporum species complex associated with Fusarium wilt of common bean in Brazil

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