Chemical diversity of essential oils from native populations of Eplingiella fruticosa

Silva, Dennis Crystian; Blank, Arie Fitzgerald; Nizio, Daniela Aparecida de Castro; Sampaio, Taís Santos; Nogueira, Paulo César de Lima

doi:10.1590/1984-70332018v18n2n29

Cited by 8 publications

(7 citation statements)

References 16 publications

(19 reference statements)

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“…In general, essential oils present significant yield, and their composition can vary depending on climatic and environmental factors, such as temperature, humidity, precipitation, soil, and time of collection. Additionally, the part of the plant used (leaves, bark, seeds or root), as well as the method of the extraction, may interfere with the composition of the essential oil of a given species [15,32,33].…”

Section: Discussionmentioning

confidence: 99%

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

et al. 2020

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Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating properties of the essential oil Ocotea odorífera (EOOO) and safrole against efflux pump (EP)-carrying strains. The EOOO was extracted by hydrodistillation, and the phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GC-MS). The antibacterial and antibiotic-modulating activities of the EOOO and safrole against resistant strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were analyzed through the broth microdilution method. The EP-inhibiting potential of safrole in association with ethidium bromide or antibiotics was evaluated using the S. aureus 1199B and K2068 strains, which carry genes encoding efflux proteins associated with antibiotic resistance to norfloxacin and ciprofloxacin, respectively. A reduction in the MIC of ethidium bromide or antibiotics was used as a parameter of EP inhibition. The phytochemical analysis identified 16 different compounds in the EOOO including safrole as the principal constituent. While the EOOO and safrole exerted clinically relevant antibacterial effects against S. aureus only, they potentiated the antibacterial activity of norfloxacin against all strains evaluated by our study. The ethidium bromide and antibiotic assays using the strains of S. aureus SA1119B and K2068, as well as molecular docking analysis, indicated that safrole inhibits the NorA and MepA efflux pumps in S. aureus. In conclusion, Ocotea odorifera and safrole presented promising antibacterial and antibiotic-enhancing properties, which should be explored in the development of drugs to combat antibacterial resistance, especially in strains bearing genes encoding efflux proteins.

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Section: Discussionmentioning

confidence: 99%

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

et al. 2020

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“…The essential oils of E. fruticosa are characterized by the variability in their components (Silva et al, 2018). This chemical polymorphism results from diverse factors, including genetic and environmental effects that, in combination or independently, affect their composition and quantitative and qualitative properties.…”

Section: Discussionmentioning

confidence: 99%

“…An injection volume of 0.5 µL (5 mg.mL -1 ) was used, with a split ratio of 1:10. Programming of oven temperature began at 50°C (isotherm for 1.5 min), with an increase of 4 °C.min -1 at 200°C, followed by 10 °C.min -1 up to 250°C, finishing with an isotherm of 5 min at 250°C (Silva et al, 2018).…”

Section: Extraction and Identification Of The Chemical Composition Of The Essential Oilsmentioning

confidence: 99%

“…The ions were generated at 70 eV at a scanning Research, Society and Development, v. 10, n. 16, e341101623723, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i16.23723 speed of 0.3 fragments (scans) s -1 detected in the interval of 40-350 Da. The FID temperature was adjusted to 250ºC, and the gases supplied to the FID were synthetic air, hydrogen, and helium at flow rates of 30, 300, and 30 mL.min -1 , respectively (Silva et al, 2018).…”

Section: Extraction and Identification Of The Chemical Composition Of The Essential Oilsmentioning

confidence: 99%

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Essential oils of Eplingiella fruticosa populations: chemical, antioxidant, and cytotoxic analyses

Pinto

Oliveira

Santos

et al. 2021

RSD

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This study investigates the variations in the chemical profiles and biological activities (antioxidant and cytotoxic) of Eplingiella fruticosa from the state of Sergipe, an endemic species from the Northeast region of Brazil. The essential oils were extracted from six populations by hydrodistillation and analyzed by GC/MS-FID. Cluster analysis was performed with the data of the constituents of the essential oils, and then a dissimilarity matrix, based on Euclidean distances, and a dendrogram, through the Ward clustering method, were constructed. The antioxidant activity of the essential oils was tested by different assays (DPPH, ABTS, β-carotene, and FRAP), and cytotoxic activity was tested by the SRB assay. The compounds found in greater amounts were α-pinene, β-pinene, 1,8-cineole, camphor, borneol, δ-elemene, α-cubebene, α-ylangene, (E)-caryophyllene, germacrene D, bicyclogermacrene, trans-calamenene, spathulenol, caryophyllene oxide, and viridiflorol. These compounds defined the formation of two groups. The first group was composed of the populations of São Cristóvão, Itaporanga, Japaratuba, and Malhada dos Bois municipalities and was characterized by the presence of the monoterpene camphor (8.39-11.27%) as the compound of greatest concentration in relation to the other municipal areas. The second group was composed of the populations of Moita Bonita and Pirambu municipalities and was characterized by the major presence of the sesquiterpene bicyclogermacrene (7.45% and 10.98%). The plants exhibited weak effects in terms of antioxidant activity; however, the essential oil showed significant toxicity for the lines A549 (51.00% cell viability) in the population of Japaratuba, and B16F10 (64.94% cell viability) in Malhada dos Bois. The observations of this study may open a way to optimize the use of the E. fruticosa populations in relation to their cytotoxic properties.

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“…In addition, it is emphasized that several authors, such as Krycki et al (2016), Costa et al (2018), Fachinetto et al (2018), Tolomeotti et al (2018) and Silva et al (2018), have carried out studies involving native species of economic importance, such as the present study.…”

Section: Meiotic Index and Pollen Viabilitymentioning

confidence: 94%

Cytogenetic characterization of Angelonia integerrima Sprengel, a native species with ornamental potential

Tedesco

Emer

Winhelmann

et al. 2019

Crop Breed. Appl. Biotechnol.

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Angelonia integerrima Sprengel is a native species of the state of Mato Grosso do Sul and the Southern region (Paraná, Santa Catarina and Rio Grande do Sul) of Brazil, with features such as an unusual appearance and color of the flowers, indicating an ornamental use. To optimize the use of this species and to fill in gaps regarding its cytogenetic characterization, this study determined the chromosome number, meiotic index and pollen viability of plant individuals of four A. integerrima populations. All plant individuals of the four populations had 2n = 20 chromosomes. Still, the meiotic index of most analyzed plant individuals exceeded 90%, while pollen viability of all plant individuals was higher than 80%. These data suggest considerable cytological stability of the analyzed A. integerrima plant individuals, which may favor the selection of future genotypes for commercial purposes or their use in conservation and breeding programs of the species.

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Chemical diversity of essential oils from native populations of Eplingiella fruticosa

Cited by 8 publications

References 16 publications

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

Essential oils of Eplingiella fruticosa populations: chemical, antioxidant, and cytotoxic analyses

Cytogenetic characterization of Angelonia integerrima Sprengel, a native species with ornamental potential

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