Genetic diversity in Brazilian soybean germplasm

Gwinner, Raoni; Setotaw, Tesfahun Alemu; Pasqual, Moacir; Santos, João Bosco dos; Zuffo, Alan Mário; Zambiazzi, Everton Vinícius; Bruzi, Adriano Teodoro

doi:10.1590/1984-70332017v17n4a56

Cited by 12 publications

(16 citation statements)

References 28 publications

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“…Data for genes that were not classified as differentially expressed are plotted in blue. In green and red, we plotted data for genes that are differentially expressed after application with glyphosate-based herbicide (Bonferroni corrected p-value ≤ 0.05) with an absolute log2 fold change (|FC|) greater than 1.5 genetic base of all commercial cultivars released in Brazil [35,36].…”

Section: Transcriptome Assembly and Gene Expressionmentioning

confidence: 99%

Stacked genetically modified soybean harboring herbicide resistance and insecticide rCry1Ac shows strong defense and redox homeostasis disturbance after glyphosate-based herbicide application

et al. 2020

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Background World agricultural production of genetically modified (GM) products, in particular, the combination of different traits/genes in the same plant has been a trend over the last decade. There have been concerns raised over stacking multiple herbicide and insect-resistant transgenes that could result in fitness costs depending on the type and strength of selection pressures exerted by the environment. Here, we report the results of transcriptomic analysis comparing the effect of glyphosate-based herbicide (GBH) in the single-transgene versus stacked, herbicide-resistant soybean varieties on various biological processes, metabolic pathways, and key shikimic enzymes. Results Gene expression data showed that defense metabolism and redox homeostasis were equally modulated in single-transgene and stacked-variety samples. Carbon accumulation and energy metabolisms were distinct between the varieties and photosynthesis metabolism was found negatively affected in the single-transgene variety only. In the stacked variety, the shikimate pathway was modulated by the accumulation of transcripts from phenylalanine gene and other cascade genes. As expected, the expression of native EPSPS was upregulated in both varieties when herbicide was applied. On the other hand, transgenic EPSPS expression was down-regulated in both GM varieties upon herbicide application which cannot be explained. Conclusion Glyphosate-based herbicides toxicity suggests its effects on carbon central metabolism and flux, redox metabolism, photosynthesis, and to hormone and defense response in plants. The observed unintended effects in GM herbicide-tolerant varieties unravel the deleterious effects previously observed on GM-tolerant varieties growth and production. The impact of GBH on shikimate and cascade pathways was observed in terms of both native and transgenic insensitive EPSPS modulation, alteration of jasmonic acid and lignin metabolism in both single-transgene and stacked variety. The energy metabolism and carbon flux were differently affected in these varieties. Oxidative stress, more specifically glutathione metabolism, induced by GBH, was also observed in this study. The stacked variety showed a more pronounced stress response (activation of specific stress defense proteins, Rboh, WRKY) and secondary compounds (β-glucosidase, isoflavone 7-O-methyltransferase). Omics profiling techniques, such as transcriptomics, can be considered tools to support risk assessment in detecting unintended effects due to the GBH application.

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Section: Transcriptome Assembly and Gene Expressionmentioning

confidence: 99%

Stacked genetically modified soybean harboring herbicide resistance and insecticide rCry1Ac shows strong defense and redox homeostasis disturbance after glyphosate-based herbicide application

et al. 2020

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“…phenotypic or molecular data). For example, in Brazil the cultivated area of soybean increased drastically, the level of genetic diversity in the soybean collection was low (Gwinner et al 2017). Nevertheless, these methods could lay foundation on the study by van Hintum (2000).…”

Section: Introductionmentioning

confidence: 99%

Development of a mulberry core collection originated in China to enhance germplasm conservation

Zhāng

Zuo

et al. 2019

Crop Breed. Appl. Biotechnol.

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China, origin of mulberry, has rich genetic resources usually. High expense, limited space and wavering environment of usually conservation in vivo poses dangerous situation for mulberry. The concept of core collection could takes priority for conservation of mulberry. In this study, 560 accessions were used with 40 morphological descriptors and stratified sampling strategies for a core collection. The core collection consisted of 28 accessions, accounting for 5% of the whole collection. The core collection included seven accessions belonging to Morus alba, one accession belonging to M. alba var. macrophylla, four accessions belonging to M. atropurprea, one accession belonging to M. nigra, three accessions belonging to M. australis, seven accessions belonging to M. multicaulis, two accessions belonging to M. wittorum and three accessions belonging to M. bombycis. The quality of core collection exceeded the evaluation criteria and could be a prioritized collection for high efficient and long-term conservation for mulberry.

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“…Although no precise information regarding the genetic background of the two commercial hybrids used were obtained, such result is expected once the soybean genetic diversity in Brazil is relatively low. For example, most of the Brazilian soybean germplasm is derived from four main genotypes (CNS, S-100, Roanoke and Tokyo), which contributed to more than a half of the genetic base of all commercial cultivars released in Brazil [35] [36].…”

Section: Validation Of Gene Expression Patterns By Rt-qpcrmentioning

confidence: 99%

Stacked genetically modified soybean harboring herbicide resistance and insecticide rCry1Ac shows strong defense and redox homeostasis disturbance after glyphosate-based herbicide application

Zanatta

Benevenuto

Nodari

et al. 2020

Preprint

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Background World agricultural production of genetically modified (GM) products, in particular, the combination of different traits/genes in the same plant has been a trend over the last decade. There has been concerns raised over stacking multiple herbicide and insect-resistant transgenes that could result in fitness costs depending on the type and strength of selection pressures exerted by the environment. Here, we report the results of transcriptomic analysis comparing the effect of glyphosate-based herbicide (GBH) in the single-transgene versus stacked, herbicide resistant soybean varieties on various biological processes, metabolic pathways, and key shikimic enzymes.Results Gene expression data showed that defense metabolism and redox homeostasis were equally modulated in single-transgene and stacked variety samples. Carbon accumulation and energy metabolisms were distinct between the varieties and photosynthesis metabolism was found negatively affected in the single-transgene variety only. In the stacked variety, the shikimate pathway was modulated by the accumulation of transcripts from phenylalanine gene and other cascade genes. As expected, the expression of native EPSPS was upregulated in both varieties when herbicide was applied. On the other hand, transgenic EPSPS expression was down regulated in both GM varieties upon herbicide application which cannot be explained. Conclusion Glyphosate-based herbicides toxicity suggests its effects on carbon central metabolism and flux, redox metabolism, photosynthesis, and to hormone and defense response in plants. The observed unintended effects in GM herbicide-tolerant varieties unravels the deleterious effects previously observed on GM tolerant varieties growth and production. The impact of GBH on shikimate and cascade pathways was observed in terms of both native and transgenic insensitive EPSPS modulation, alteration of jasmonic acid and lignin metabolism in both single-transgene and stacked variety. The energy metabolism and carbon flux were differently affected in these varieties. Oxidative stress, more specifically glutathione metabolism, induced by GBH, was also observed in this study. The stacked variety showed a more pronounced stress response (activation of specific stress defense proteins, Rboh, WRKY) and secondary compounds (β-glucosidase, isoflavone 7-O-methyltransferase). Omics profiling techniques, such as transcriptomics, can be considered tools to support risk assessment in detecting unintended effects due to the GBH application.

show abstract

Genetic diversity in Brazilian soybean germplasm

Cited by 12 publications

References 28 publications

Stacked genetically modified soybean harboring herbicide resistance and insecticide rCry1Ac shows strong defense and redox homeostasis disturbance after glyphosate-based herbicide application

Stacked genetically modified soybean harboring herbicide resistance and insecticide rCry1Ac shows strong defense and redox homeostasis disturbance after glyphosate-based herbicide application

Development of a mulberry core collection originated in China to enhance germplasm conservation

Stacked genetically modified soybean harboring herbicide resistance and insecticide rCry1Ac shows strong defense and redox homeostasis disturbance after glyphosate-based herbicide application

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