Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction

Kumar, Suresh; Saxena, Sheena; Gupta, M. G.

doi:10.1590/1984-70332017v17n1a2

Cited by 12 publications

(24 citation statements)

References 25 publications

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“…Progenies of 198 hybrid plants were established for agronomic assessment and the 28 most productive plants in terms of herbage accumulation and production of leaves were selected. Cytoembryological analyses and molecular markers were used to assess the reproduction mode of the hybrids, indicating segregation for apomictic and sexual genotypes (Weiler et al 2017), also reported by Kumar et al (2010b) and Kumar et al (2017) in Cenchrus ciliaris, which reinforces the possibility of producing apomictic cultivars with desirable traits and using sexual genotypes as mother plants in future crosses. Barbosa et al (2019) established them in lines and evaluated in two physiographic regions of Rio Grande do Sul (Central Depression and Campain), showing the productive potential of some of these genotypes.…”

Section: Introductionsupporting

confidence: 61%

“…The native germplasm is tetraploid and displays apomictic reproduction (Forbes and Burton 1961). By the apomictic reproduction mode, the seeds are clones of the mother plant (Ortiz et al 2013), which can be either a drawback or a valuable tool for crop improvement (Kumar et al 2017). Historically, the breeding of apomictic plants was limited by selection, evaluation and natural perception of the occurrence of the most vigorous and highest-yielding apomictic ecotypes (Miles 2007).…”

Section: Introductionmentioning

confidence: 99%

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Reproductive analyses of intraspecific Paspalum notatum Flügge hybrids.

Krycki

Tedesco

Rodrigues

et al. 2020

Crop Breed. Appl. Biotechnol.

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Paspalum notatum is an important forage native to Southern Brazil and one of the most promising fodder species in terms of productivity, quality, resistance and growth speed. The objective of this study was to evaluate the reproductive mode and fertility of hybrid progenies resulting from artificial crosses among artificially duplicated sexual plants, selected intraspecific hybrids of the UFRGS breeding program and apomictic ecotypes of the species. The resulting F 1 progenies were represented by 24 plants with high pollen viability (varying from 82.15 to 99.67%) and 1.2:1 segregation for the sexual: apomictic reproduction mode, indicating predominantly sexual hybrids, due to distorted segregation. In the apomictic plants, most chromosomes paired as bivalents in diakinesis. Since the low presence of abnormalities observed in the meiotic chromosome pairing and the high pollen viability of the hybrids, all plants were confirmed as promising parents for future crosses.

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Section: Introductionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Reproductive analyses of intraspecific Paspalum notatum Flügge hybrids.

Krycki

Tedesco

Rodrigues

et al. 2020

Crop Breed. Appl. Biotechnol.

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“…This difference may be due to an expression of apospory in these hybrids, which was not detected by flow cytometry; therefore, a greater number of seeds should be analyzed. Several attempts have been made to develop molecular markers that can be used to discriminate between sexual and apomictic genotypes resulting from crosses in other genera, (e.g., Brachiaria, Poa, Cenchrus, and Panicum;Pessino et al, 1997;1998;Albertini et al, 2001;Dwivedi et al, 2007;Zorzatto et al, 2010;Bluma Marques et al, 2014;Worthington et al, 2016;Kumar et al, 2017). However, few reports of the use of these markers are available.…”

Section: Comparison Between Sexual and Apomictic Hybrids For Morpholomentioning

confidence: 99%

Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts, and Impact of Apomixis on Hybrid Performance

et al. 2019

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Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identification of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male‐specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis‐linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids.

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“…This approach provides very useful information from gene expression to cell metabolism and genetic linkages, and has consequently become a link among genomics, genetics, and physiology. Unlike the widely used DNA molecular markers in plant genetic research and breeding (Garrido-Cardenas et al 2017, Kumar et al 2017, proteomic polymorphisms based on 2-DE are real-time expression markers; therefore, the protein markers are beneficial for defining the relationships of molecular genetic markers with their physiological function. Furthermore, the method can generate novel insight and be useful for crop improvement.…”

Section: Introductionmentioning

confidence: 99%

Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis

Chen

Jiang

Huang

et al. 2019

Crop Breed. Appl. Biotechnol.

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To identify potential protein markers associated with tillering in rice, a d10 allelic mutant (JHCA) with a high-tillering dwarf phenotype and a wild-type cultivar (GLA4) with normal numbers of tillers were used to produce a hybrid (F 1 ), and 2-DE (two-dimensional electrophoresis) analysis was conducted in seedling leaf sheaths and roots of the rice hybrid and its parents. A total of 61 protein spots representing qualitative or quantitative polymorphisms were detected. Among them, 38 spots were successfully identified as 23 different proteins by MALDI-TOF/MS. The identified proteins were further divided into P/As (presence/absence variations), PSs (position shift variations) and qVs (quantitative variations). Most of the identified polymorphic proteins exhibited tissue-specific expression. Two spot families, identified as a putative vitamin B12-independent methionine synthase and a putative phosphoglycerate mutase were found. The profiles of expression abundance of three iso-spots in each spot family showed a reciprocal relationship between the two parents.

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Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction

Abstract: Cenchrus ciliaris L. is an apomictic forage grass grown in pastures and

Cited by 12 publications

References 25 publications

Reproductive analyses of intraspecific Paspalum notatum Flügge hybrids.

Reproductive analyses of intraspecific Paspalum notatum Flügge hybrids.

Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts, and Impact of Apomixis on Hybrid Performance

Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis

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