Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil

Abstract

-Mandevilla guanabarica is an endemic plant from Brazil, with pharmacological and ornamental potential, both unexplored

GC-MS Analysis of the in vitro leaf derived Callus Extracts of Ichnocarpus frutescens (L)W.T.Aiton

“…But in different concentrations of IBA, BAP, Kn did not induce callus from the leaf explants and also in hormone-free MS medium. Similar results were recorded in Mandevilla guanabarica, 40 µM/L 2, 4-dichloro phenoxy acetic acid (2, 4-D) induced compact creamish color, non-morphogenic callus from leaf explants on MS media 23 . The embryogenic callus was formed from mature embryos of winter Wheat on MS media fortified with 2,4-D as a effective growth regulator for higher callus induction than NAA hormone 24 .…”

An efficient shoot regeneration system for medicinally important Elephantopus scaber Linn.

“…Ninety eight per cent of the cultures produced multiple shoots with an average of 33.7 shoots per explant when 20-day-old cotyledonary node explants were cultured on MS medium supplemented with 1.5 mg/l TDZ and 0.5 mg L -1 NAA after 45 d of culture ( Figure 2A; Table 1). The superiority of TDZ, a substituted phenyl urea, over other adenine type cytokinins for shoot induction in cultures has been well documented in several systems (Cheruvathur et al 2013, Cordeiro et al 2014. In the present study, a combination of TDZ and NAA produced the highest response.…”

In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

“…Shoot formation from calli has been previously achieved by cytokinin or auxin supplementation or by varying the mineral concentration in the medium (Kour et al 2014). The immense activity of BAP compared to other cytokinins is reported in many plants, including Salvia officinalis (Tawfik and Mohamed 2007), Thymus bleicherianus (Aicha and Abdelmalek 2014), and Mandevilla guanabarica (Zorat Cordeiro et al 2014). As shown in Table 2, calli cultured on PGR-free MS medium exhibited a high shoot formation rate (78%).…”