GC-MS Analysis of the in vitro leaf derived Callus Extracts of Ichnocarpus frutescens (L)W.T.Aiton

Abstract

Medicinal plants are the treasure house of secondary metabolites. Production of seconmdary metabolites through micro propagation technology has immense application in new drug discoveries. Ichnocarpus frutescens (L.) W.T. Aiton - is a latex secreting medicinal herb commonly known as black creeper or Krishna Sariva belonging to the family Apocyanaceae.The present study was aimed at induction of callus from the leaf explants of I. frutescens using Murashige and Skoog (MS) medium supplemented with various combinations of plant growth regulators. Maximum amount of green compact mass of callus was proliferated from leaf explants on MS medium fortified with 3mg/L NAA + 2mg/L BAP. The main objective of the present study was to investigate the secondary metabolites in different extracts of callus by GC-MS analysis. To our best knowledge, no literature is available and this is the first report on GC–MS analysis of leaf derived callus extracts, which showed the presence of different phytocompounds out of which, Stigmasterol, Hexadecanoic acid, Beta-Sitosterol, Stigmasta-3,5-dien-7-one and Heptacosane are the major compounds that found to possess a wide range of biological activities. This protocol could be used to identify the secondary metabolites from leaf derived callus of the I. frutescens and further studies have to be carried out for the isolation and characterization of novel compounds for the formulation of new pharmaceutical drugs and also for its commercial production which will be used to cure some important ailments.

Optimization of the alkali treatment on the tempo‐modified cellulosic nanoreinforcement in autoclaved epoxy matrix

“…Relatively, the surfaces of the composite films subjected to 5 wt% alkali treatment are significantly more rigid and rough, measuring a mean roughness of 85.23 nm. This demonstrates complete molecular mixing of seed filler, which eventually improves the interface relationship with the epoxy matrix 96 . Meanwhile, 10 wt% alkali‐treated filler composites show lower roughness of 66.56 nm because of the damaged and void‐infused composites 97 …”

“…As depicted in Figure 4D of 10 wt% treated TMCF‐epoxy composites where the filler aggregated, resulting in poor interlocking between the polymer and the filler. The higher concentration (10 wt%) leads to decline in properties, which might be because excess concentration delignify the fiber excessively that can adversely affect the strength of fiber that ultimately affect the mechanical properties of the nano‐composites 95–97 . So, 5 wt% of treated composites show better mechanical properties and outperformed other composites and autoclaved epoxy 99 .…”

“…Similar outcomes of other natural fibers like Sansevieria ehrenbergii (52.27%), 94 Heteropogan contortus . (54.1%), 95 A. planifrons (51.72%), 46 Sida rhombifolia (56.6%), 96 and Dichrostachys cinerea (57.82%), 97 and so forth. were found in the literature.…”