TEST OF EXUDATE pH IN RICE SEEDS

Abstract

The objective of this study was to evaluate the efficiency of a pH test of the exudate (with and without tegument) as an indicator of the physiological quality of rice seeds. The experimental design was completely randomized, with seven lots and four replications. The seven lots were assessed for water content and initial physiological quality by means of germination, first germination count, emergence, emergence speed index and tetrazolium tests. The pH test of the exudate was conducted with 100 seeds of each treatment, with tegument and without tegument. The seeds were individually soaked in 2.0 mL distilled water for different periods (20, 40, and 60 minutes) and three temperatures (20, 25, and 30 °C). The results were submitted to the Scott Knott test at 5% probability. The pH test of the exudate is a promising method to evaluate the physiological quality of rice seeds by detecting differences in vigor between marketable lots. This test should be conducted with rice seed without tegument, at 25 °C, with a soak time of 60 minutes.

Viability of melon seeds using the pH testing of exudate

“…However, during 90 minutes of soaking, the lots could be stratified similarly to the results obtained for the first count, emergence, emergence speed index, accelerated aging, and tetrazolium tests, with lots 1, 2, and 3 being classified as high, medium, and low performance, respectively. On the contrary, this test was efficient in classifying rice lots when the seeds were soaked for 60 minutes at 25 °C (Santos et al, 2020). Seeds from lots of the hybrid Premier showed different performance at the temperature of 30 °C than those verified at 25 °C for periods of 30, 60, and 90 minutes.…”

“…Research using the pH testing of exudate has already been carried out for several agricultural species, with promising results in soybean (Amaral and Peske, 1984), pea (Rech et al, 1999), crambe (Alves et al, 2016), rice (Santos et al, 2020), and wheat (Grzybowski et al, 2022). Considering that there is no research using this test on melon seeds, this study aimed to evaluate the effectiveness of this rapid viability method on seeds of this fruit vegetable.…”