Evaluación preclínica y estudio de estabilidad de extractos a partir del follaje de Momordica charantia Lin

Abstract

Evaluación preclínica y estudio de estabilidad de extractos a partir del follaje de RESUMEN:El objetivo del presente trabajo fue la evaluación preclínica y el estudio de estabilidad de extractos a partir del follaje de Momordica charantia Lin. Se obtuvieron extractos acuoso e hidroalcohólico para los cuales se establecieron las especificaciones de calidad mediante la evaluación de tres lotes y se estudió su estabilidad por el método de vida de estante durante 12 meses. A los extractos se le evaluó el potencial genotóxico mediante ensayos de micronúcleos en médula ósea de ratón y aberraciones cromosómicas en linfocitos de sangre periférica. La actividad hipoglicemiante oral fue evaluada en animales con hiperglicemia temporal inducida por carga de glucosa. Como resultados se establecieron las especificaciones de calidad de los extractos acuoso e hidroalcohólico, los mismos mostraron estabilidad por 6 meses para el extracto acuoso y 12 meses para el extracto hidroalcohólico. No mostraron efecto genotóxico en los ensayos evaluados y mostraron efecto hipoglicemiante oral a la dosis de 450 mg/kg. Palabras clave:Momordica charantia Lin, extractos, estabilidad, genotoxicidad, hipoglicemiante. ABSTRACT: Obtaining and characterizing the Momordica charantia Linn leaf extracts.The objective of this investigation was the preclinical evaluation and the stability study of the Momordica charantia Linn hydroalcoholic and aqueous leaf extracts. The hydroalcoholic and aqueous extracts were obtained and the quality specifications were determined by evaluating three lots. The stability of the extracts was evaluated for 12 months. The genotoxic potential of the extracts was evaluated by mouse bone marrow micronucleus test and chromosome aberration test. The hypoglycemic effect was determined by oral glucose tolerance test. As results, the quality specifications were established and the aqueous extract was stable for 6 months and the hydroalcoholic extract for 12 months. A genotoxic effect was not observed in both extracts and the hypoglycemic effect was observed at the oral dose of 450 mg/kg of body weight.

Antidiabetic, hypolipidemic, antioxidant and anti-inflammatory effects of Momordica charantia L. foliage extract

“…Solar dryer was used to dry the plant material. The aqueous extract was obtained as described previously (Lagarto et al, 2014). A Büchi B191 spray dryer (Flawil, Switzerland) was used for drying the extract.…”

“…The pharmacological properties of the fruits have been extensively studied but there are very few reports concerning the effects and safety of the M. charantia foliage extracts. Supported by the traditional use of the plant and the reported properties for diabetes treatment (Alam et al, 2015;Krawinkel and Keding, 2006), we have proposed the design of a phytopharmaceutical using M. charantia foliage extract desiccated by a spray-drying technique as an active pharmaceutical ingredient (Lagarto et al, 2008;2014). This technological intervention improved the physic-chemical and stability properties of the extract (Lagarto et al, 2008;2014), but could interfere with its pharmacological properties.…”

“…Supported by the traditional use of the plant and the reported properties for diabetes treatment (Alam et al, 2015;Krawinkel and Keding, 2006), we have proposed the design of a phytopharmaceutical using M. charantia foliage extract desiccated by a spray-drying technique as an active pharmaceutical ingredient (Lagarto et al, 2008;2014). This technological intervention improved the physic-chemical and stability properties of the extract (Lagarto et al, 2008;2014), but could interfere with its pharmacological properties. Here we aimed to evaluate the metabolic effects of this powdered M. charantia foliage extract (MCh) on murine models of diabetes by determining blood glucose and lipids status.…”

Evaluation of Commonly Used Surinamese Medicinal Plants for Their Potential Cytotoxic and Genotoxic Effects Using Cultured Chinese Hamster Ovary Cells

“…displayed antitumor activity in laboratory animals [52] and were genotoxic in wing spot test [53] n a mouse bone marrow micronucleus test and a chromosome aberration test [54]. And an ethanolic extract from S. mombin leaves displayed an antifertility effect in laboratory rats [55] while a hydromethanol extract increased the formation of micronucleated polychromatic erythrocytes in Swiss albino mice [56].…”