Fish biomarker responses to perturbation by drought in streams

Ondei, Luciana de Souza; Teresa, Fabrício Barreto; Garcia, Danielly; Felício, Andréia Arantes; Silva, Danilo Grünig Humberto da; Almeida, Eduardo Alves de

doi:10.1590/1982-0224-2019-0130

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…Similarly, in tambaqui (Colossoma macropomum), Nile tilapia (Oreochromis niloticus), Amazon catfish (Pseudoplatystoma reticulatum), and pacu (Piaractus mesopotamicus), alkaline pH present more deleterious effects than acidic pH [93][94][95]. However, the deterioration of water quality in extreme drought conditions during the dry season (summer season) causes oxidative stress and enhanced peroxidation of lipids as fish are subjected to hypoxia under these conditions [96]. The present results showed the highest gills, liver, and muscle MDA levels during the winter season.…”

Section: Oxidative Stress Responsesmentioning

confidence: 99%

Integrated use of biomarkers to assess the impact of heavy metal pollution on Solea aegyptiaca fish in Lake Qarun

et al. 2022

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Background Biomarkers have become a valuable tool in environmental assessment, since they contribute to predicting contaminants in monitoring programmes. This study aimed to investigate the toxicity of heavy metal pollution in Lake Qarun using a multibiomarker approach (morphological, oxidative stress, genotoxicity, stress proteins) in Solea aegyptiaca fish. During the winter and summer seasons, water and fish samples were collected from different locations along Lake Qarun; western and northern sectors were away from any source of pollution, while southern and eastern sectors were exposed to effluents discharged from El-Wadi and El-Bats drains, respectively. The environmental quality, as well as the accumulation of metals (Fe, Cu, Zn, Cd, Pb, Ni) in fish gills, liver, and muscles were assessed. Data were integrated using the integrated biomarker response index (IBRv2) for biomarker response interpretation. Results Water quality and bioaccumulation of heavy metals revealed a highly significant difference between samples collected from polluted sectors and those collected far from drainage water, and, seasonal differences were detected. Growth indices revealed a significant difference between sites and seasons. Fish from the western sector had the highest total antioxidant capacity in their gills, liver, and muscles, with no seasonal differences detected. However, the maximum value of malondialdehyde, protein carbonyl, 8-hydroxy-2′deoxyguanosine, metallothionein, heat shock protein 70, and DNA strand breaks in gills, liver, and muscles was detected in the polluting sectors. It revealed a significant difference between seasons, with the highest value during the winter season. According to IBRv2 results, the most effective biomarkers in this study were malondialdehyde and 8-hydroxy-2′deoxyguanosine in gills, protein carbonyl and metallothionein in the liver, heat shock protein 70 in gills and liver, and DNA strand break in gills, liver, and muscles. Conclusions This multibiomarker approach contributes to distinguishing between locations with varying levels of anthropogenic pollution, identifying the drainage water-exposed sectors as the most stressed and the winter season as the most critical time for Solea aegyptiaca owing to spawning. The biomarkers chosen are effective indicators in Solea aegyptiaca under stress, indicating the potential for environmental monitoring. Graphical Abstract

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Section: Oxidative Stress Responsesmentioning

confidence: 99%

Integrated use of biomarkers to assess the impact of heavy metal pollution on Solea aegyptiaca fish in Lake Qarun

et al. 2022

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“…For example, mussels Brachidontes solisianus stimulate glutathione synthesis after 4h of air exposure (a condition that impairs oxygen uptake) in a rocky beach under natural settings ( Moreira et al, 2021 ). In the case of Astyanax elachylepis , fish collected in a naturally hypoxic intermittent stream suffer more oxidative damage and have increased GPX activity in gills compared with fish living in a naturally normoxic perennial stream during the dry season ( Ondei et al, 2020 ). More recently, the levels of redox metabolism biomarkers in naturally hibernating common Asian toads, Duttaphrynus melanostictus , at the end of the winter were compared with those in naturally active toads collected during summer ( Patnaik and Sahoo, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…Given the large number of studies on the modulation of antioxidants conducted with animals under artificially controlled settings, we have previously set the challenge of assessing POS under natural conditions ( Moreira et al, 2017 ). This challenge was met by few recent studies ( Ondei et al, 2020 ; Moreira et al, 2021 ; Patnaik and Sahoo, 2021 ), including one that evaluated the modulation of endogenous antioxidants in the muscle of Proceratophrys cristiceps (Anura: Odontophrynidae) from the Brazilian Caatinga ( Moreira et al, 2020 ). In addition to the scarcity of studies under field conditions, it is still not known if POS can be generalized to other anuran species estivating in semi-arid environments.…”

Section: Introductionmentioning

confidence: 99%

Metabolic and Redox Biomarkers in Skeletal Muscle Underlie Physiological Adaptations of Two Estivating Anuran Species in a South American Semi-arid Environment

Moreira

Carvajalino-Fernández

Navas

et al. 2021

Front. Physiol.

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The upregulation of endogenous antioxidants (i.e., preparation for oxidative stress, POS) is part of the biochemical responses underlying the adaptation of animals to adverse environments. Despite the phylogenetic diversity of animals in which POS has been described, most studies focus on animals under controlled laboratory conditions. To address this limitation, we have recently assessed the redox metabolism in the skeletal muscle of Proceratophrys cristiceps estivating under natural settings in the Caatinga. Here, we analyzed biochemical biomarkers in the muscle of another Caatinga species, Pleurodema diplolister, during the rainy (active) and dry (estivating frogs) seasons. We aimed to determine whether P. diplolister enhances its antioxidants during estivation under field conditions and to identify any effect of species on the biochemical responses of P. diplolister and P. cristiceps associated with estivation. To do so, we measured the activities of representative enzymes of intermediary metabolism and antioxidant systems, as well as glutathione and protein carbonyl levels, in the skeletal muscle of P. diplolister. Our findings revealed the suppression of oxidative metabolism and activation of antioxidant enzymes in estivating P. diplolister compared with active specimens. No changes in oxidative damage to proteins were observed and estivating P. diplolister had lower levels of disulfide glutathione (GSSG) and disulfide-to-total glutathione ratio (GSSG/tGSH) than those observed in active individuals. When data for P. diplolister and P. cristiceps were assembled and analyzed, significant effects of species were detected on the activities of metabolic enzymes (citrate synthase, isocitric dehydrogenase, malic enzyme, and creatine kinase) and antioxidant enzymes (catalase, glutathione peroxidase and glutathione transferase), as well as on GSSG/tGSH ratio. Such effects might underlie the physiological and behavioral differences between these two species that share the same microhabitat and survival strategy (i.e., to estivate) during the dry season. Despite some peculiarities, which reflect the physiological diversity of the mechanisms associated with estivation in the Brazilian Caatinga, both P. diplolister and P. cristiceps seem to balance the suppression of oxidative pathways, the maintenance of the capacity of oxygen-independent pathways, and the activation of endogenous antioxidants to preserve muscle function and be ready to resume activity whenever the unpredictable rainy period arrives.

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“…Since almost all POS-related studies, until 2016, were performed in the lab, we proposed a challenge to show more evidence of POS phenotype in animals under natural conditions, i.e., in the wilderness ( Moreira et al, 2017b ). Several studies met this challenge, including the investigation of fish endurance in a seasonal hypoxic stream ( Ondei et al, 2020 ), frog estivation in a semi-desertic region (two species; Moreira et al, 2020 ; Moreira et al, 2021a , toad hibernation in cold mountains in India ( Patnaik & Sahoo, 2021 ), and aerial exposure of mollusks Brachidontes solisianus and Littorina kurila during the low tide ( Istomina, Belcheva & Chelomin, 2013 ; Moreira et al, 2021b ). We also recently highlighted that ultraviolet radiation (UVR) could trigger a POS-response in several invertebrate species with a molecular mechanism similar to hypoxia-induced POS ( Geihs et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

The role of solar radiation and tidal emersion on oxidative stress and glutathione synthesis in mussels exposed to air

Moreira

Sabino

Minari

et al. 2023

PeerJ

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Preparation for oxidative stress (POS) is a widespread adaptive response to harsh environmental conditions, whose hallmark is the upregulation of antioxidants. In contrast to controlled laboratory settings, animals are exposed to multiple abiotic stressors under natural field conditions. Still, the interplay between different environmental factors in modulating redox metabolism in natural settings remains largely unexplored. Here, we aim to shed light on this topic by assessing changes in redox metabolism in the mussel Brachidontes solisianus naturally exposed to a tidal cycle. We compared the redox biochemical response of mussels under six different natural conditions in the field along two consecutive days. These conditions differ in terms of chronology, immersion/emersion, and solar radiation, but not in terms of temperature. Animals were collected after being exposed to air early morning (7:30), immersed during late morning and afternoon (8:45–15:30), and then exposed to air again late afternoon towards evening (17:45–21:25), in two days. Whole body homogenates were used to measure the activity of antioxidant (catalase, glutathione transferase and glutathione reductase) and metabolic (glucose 6-phosphate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase and pyruvate kinase) enzymes, reduced (GSH) and disulfide (GSSG) glutathione levels, and oxidative stress markers (protein carbonyl and thiobarbituric acid reactive substances). Air and water temperature remained stable between 22.5 °C and 26 °C during both days. Global solar radiation (GSR) greatly differed between days, with a cumulative GSR of 15,381 kJ/m2 for day 1 and 5,489 kJ/m2 for day 2, whose peaks were 2,240 kJ/m2/h at 14:00 on day 1 and 952 kJ/m2/h at 12:00 on day 2. Compared with animals underwater, emersion during early morning did not elicit any alteration in redox biomarkers in both days. Air exposure for 4 h in the late afternoon towards evening caused oxidative damage to proteins and lipids and elicited GSH synthesis in animals that had been previously exposed to high GSR during the day. In the following day, when GSR was much lower, exposure to air under the same conditions (duration, time, and temperature) had no effect on any redox biomarker. These findings suggest that air exposure under low-intensity solar radiation is not sufficient to trigger POS in B. solisianus in its natural habitat. Thus, natural UV radiation is possibly a key environmental factor that combined to air exposure induces the POS-response to the stressful event of tidal variation in this coastal species.

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Fish biomarker responses to perturbation by drought in streams

Cited by 6 publications

References 47 publications

Integrated use of biomarkers to assess the impact of heavy metal pollution on Solea aegyptiaca fish in Lake Qarun

Integrated use of biomarkers to assess the impact of heavy metal pollution on Solea aegyptiaca fish in Lake Qarun

Metabolic and Redox Biomarkers in Skeletal Muscle Underlie Physiological Adaptations of Two Estivating Anuran Species in a South American Semi-arid Environment

The role of solar radiation and tidal emersion on oxidative stress and glutathione synthesis in mussels exposed to air

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