Treatment of sugarcane bagasse for the immobilization of soybean β-glucosidase and application in soymilk isoflavones

Moreira, Amanda Aleixo; Ferreira, Ana Camila Vaitkevicius; Silva, Josemeyre Bonifácio da; Ribeiro, Mara Lúcia Luiz

doi:10.1590/1981-6723.24318

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…The immobilized enzyme yield on bagasse fibre matrix, remained highest (pectinase-43.12%, cellulase 74.5% and amylase 50.3%) after 5 months of storage at -20°C as compared to rice husk, wheat straw and paddy straw. Moreira et al (2019) reported the bagasse treatments modify the morphological structures, influence the compositions of the minerals and the chemical groups of the fibers and enable the enzyme immobilization. The thermal stability of the immobilized enzymes is greater than that of solubilized enzymes, and it is possible to reuse the same immobilized structure for 15 cycles without a complete loss of activity.…”

Section: Molecular Identification Of Selected Bacteriamentioning

confidence: 99%

Production of multi enzyme preparation by Bacillus subtilis using mosambi peel as substrate

Singh¹,

Garg²,

Mathur³

et al. 2022

JEB

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Aim: To determine feasibility of using mosambi peel as substrate for multi enzyme production through microbial intervention. Methodology: Thirty-three bacterial isolates were isolated from biodegradable organic substrates and investigated in-vitro their biodegradable activities viz. pectinolytic, cellulolytic and amylolytic. The best performing bacterial isolate, exhibiting aforesaid activities were selected and identified using 16S rRNA technique. The factors, that influenced the fermentation, viz. temperature, pH and incubation period of bacterial culture were optimized for maximum production of multi enzymes viz. pectinase, cellulase and amylase using mosambi peel as substrate under solid-state fermentation conditions. Molecular weights of different enzymes present in multi-enzyme preparation were determined by SDS PAGE. The juice extraction efficiency of crude multi enzyme preparation was compared with that of pure commercial enzyme. Results: Out of thirty-three bacterial isolates, after primary and secondary screening, the best performing bacterial isolate was identified as Bacillus subtilis strain NG 105 (Genbank accession number MN493055). Higher enzyme activities were observed at pH 7.0, incubation temperature of 35°C and incubation period of 10 days for pectinase, cellulase and amylase using mosambi peel as substrate. The purified enzymes characterized by SDS-PAGE, revealed to have molecular mass of 65 kDa for pectinase, 50 kDa for cellulase and 55 kDa for amylase. Bagasse fibre was found to be the most suited matrix for immobilization. The juice extraction efficiency of partially purified crude multienzyme preparation was 88% of commercial pectinase. Interpretation: Mosambi peel is a suitable substrate for multienzyme production using Bacillus subtilis under solid-state fermentation condition.

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Section: Molecular Identification Of Selected Bacteriamentioning

confidence: 99%

Production of multi enzyme preparation by Bacillus subtilis using mosambi peel as substrate

Singh¹,

Garg²,

Mathur³

et al. 2022

JEB

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“…A espectroscopia na região do infravermelho médio é uma técnica utilizada na caracterização química dos constituintes presentes na amostra, pois permite elucidar e entender os tipos de ligações e grupos funcionais presentes (STANISLAV et al, 2010;MOREIRA et al, 2019). A obtenção dos espectros no infravermelho médio foi executada em um espectrofotômetro da marca Perkin Elmer IR Spectrum Two.…”

Section: Espectroscopia Na Região Do Infravermelho Médiounclassified

Caracterização da biomassa de bagaço de cana-de-açúcar com vistas energéticas

2021

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A utilização de biomassas residuais de plantas utilizadas na agroindústria tem sido crescente. O bagaço de cana-de-açúcar se destaca nesse cenário, já que a cultura da planta é muito difundida, gerando grandes quantidades desse resíduo. Neste contexto é necessário entender as características dessa biomassa para poder otimizar a geração de energia. Diante do exposto, o presente trabalho teve como objetivo caracterizar e avaliar o potencial energético da biomassa de cana-de-açúcar por meio da análise dos principais parâmetros que influenciam esse processo. Foram realizadas análises de poder calorífico superior, análises químicas imediatas, infravermelho e análise elementar, com o intuito de avaliar o potencial energético desse material. Observou-se bom nível de poder calorífico superior, 4309,50 kcal kg-1, além de teores baixos de cinzas, 0,95%, fatores que podem explicar a grande utilização da biomassa de bagaço de cana-de-açúcar para a geração de energia direta, principalmente na indústria. Palavras-chave: Resíduos. Cana-de-açúcar. Fonte de energia. Characterization of sugar cane bagasse biomass with energy views Abstract The use of residual biomass from plants used in agribusiness has been increasing. The sugarcane bagasse stands out in this scenario, since the plant's culture is very widespread, generating large amounts of this residue. In this context, it is necessary to understand the characteristics of this biomass in order to optimize the energy generation. With the above, the present study had as objective characterize and evaluate energy potential of sugarcane biomass through the analysis of the main parameters that influence the process. Analyzes of superior calorific power, immediate chemical analyzes, infrared and elementary analysis were carried out, in order to evaluate the energetic potential of this material. A good level of higher calorific value was observed, 4309.50 kcal kg-1, in addition to low ash content, 0.95%, factors that may explain the great use of sugarcane bagasse for direct energy generation, mainly in industry. Keywords: Waste. Sugarcane. Energy source.

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Environmental impact of lignocellulosic wastes and their effective exploitation as smart carriers – A drive towards greener and eco-friendlier biocatalytic systems

Bilal

Wang

Cui

et al. 2020

Science of The Total Environment

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Treatment of sugarcane bagasse for the immobilization of soybean β-glucosidase and application in soymilk isoflavones

Cited by 3 publications

References 36 publications

Production of multi enzyme preparation by Bacillus subtilis using mosambi peel as substrate

Production of multi enzyme preparation by Bacillus subtilis using mosambi peel as substrate

Caracterização da biomassa de bagaço de cana-de-açúcar com vistas energéticas

Environmental impact of lignocellulosic wastes and their effective exploitation as smart carriers – A drive towards greener and eco-friendlier biocatalytic systems

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