BIOLOGIA DE ANOFELINOS AMAZÔNICOS. XIV. ISOENZIMAS DE ESTERASE EM ANOPHELES TRIANNULATUS (NEIVA &amp; PINTO, 1922)

Santos, Joselita Maria Mendes dos; Tadei, Wanderli Pedro; Contel, E. P. B.

doi:10.1590/1809-43921992222228

Cited by 5 publications

(4 citation statements)

References 3 publications

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“…displays a high degree of morphological variation (Galvão 1941, Forattini 1962, Silva-doNascimento & Lourenço-de-Oliveira 2002. In addition to morphological variation, previous studies have shown that this malaria vector presents polymorphisms in molecular markers and exhibits behavioural variation, suggesting that it is in fact a complex of cryptic species (Santos et al 1992, Rosa-Freitas et al 1998, Silva-doNascimento & Lourenço-de-Oliveira 2002. Some interesting particularities emerged from studies of these three sympatric cryptic species.…”

Section: Discussionmentioning

confidence: 99%

Molecular divergence in the timeless and cpr genes among three sympatric cryptic species of the Anopheles triannulatus complex

Silva-do-Nascimento¹,

Rona

Peixoto

et al. 2011

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

confidence: 99%

Molecular divergence in the timeless and cpr genes among three sympatric cryptic species of the Anopheles triannulatus complex

Silva-do-Nascimento¹,

Rona

Peixoto

et al. 2011

Mem. Inst. Oswaldo Cruz

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“…Studies on the protein variability of Anopheles have shown that in this group of mosquitoes, most isoenzymatic patterns are of autosomal codominant inheritance (BIANCHI & RINALDI, 1970;BOLLINI et al, 1971a;NARANG & KITZMILLER, 197la, b;1973a, b;KAI-SER etai, 1988;NARANG etal, 1979a, b;1989a, b;SANTOS et al, 1992 Studies on the enzymatic polymoiphism of Anopheles have been performed by (SANTOS, 1979;NARANG et ai, 1979a, b;SANTOS et ai, 1985) in populations of BR-174 (Manaus/Boa Vista). These au thors found that, of the systems studied, the esterases were the most variable, with complex electrophoretic profiles involv ing six molecular forms.…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

1996

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-Of eleven proteins analyzed in four Amazonian populations, the esterases showed the greatest variation, with five activity zones. EST1, EST2 and EST5 showed variation in each of the populations studied. EST1 and EST2 are each controlled by two, and EST5 by four, codominant alleles. LAP presented six activity zones, with codominant variation in LAP5 and LAP6. oc-GPDH was monomorphic with one activity band on starch gel and two on polyacrylamide gel. 1DH presented two activity zones, with variation in the IDHl region. PGM had a single activity zone, with variation in all populations. The Ariquemes populations showed five alleles and the other populations three, all of then codominant. Three activity zones with two codominant alleles were observed for ODH. Aldehyde Oxidase showed two activity zones, with variation in AOl only in the Ariquemes and Porto Velho/Samuel populations. 6-PGDH showed only one activity zone and variation only in the Ariquemes population. The remaing systems -XDH, G-6-PDH and GDH. was monomorphic. Key words: Anopheles darlingi, isozymes, electrophoresisAnálise Eletroforética de 11 Enzimas era Populações Naturais de Anopheles (Ν.) darlingi Root, 1926 (Diptera: Cuiicidae) na Região Amazônica.RESUMO -Dos 11 sistemas protéicos analisados, em quatro populações da Amazônia, as esterases foram as que apresentaram maior variação, com cinco zonas de atividade. EST1, EST2, e EST5 mostraram variação genética determinada em todas populações estudadas, sendo que as duas primeiras são resultantes do controle de dois alelos e a EST5 de quatro, todos codominantes. A LAP consiste de seis zonas de atividade e com variação apenas nos loci LAP5 e LAP6, ambos codominantes. A GPDH mostrou-se monomórfica com uma zona de atividade em gel de amido e duas em poliacrilamida. A IDH apresentou duas zonas de atividade, com variação na região da IDH1. A PGM consiste de uma única zona de atividade e com variação em todas as populações estudadas. A população de Ariquemes revelou cinco alelos e as demais três, todos codominantes. Para a ODH, foram observadas três zonas de atividade com dois alelos codominantes. A enzima AO apresentou duas zonas de atividade, sendo que a AOl mostrou-se variável apenas em Ariquemes e Porto Velho/Samuel. 6-PGDH possui apenas uma zona de atividade e variação apenas em Ariquemes. Os demais sistemas -XDH, G-6-PDH e GDH -mostraram-se monomórficos.

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“…In populations of the cricket Gryllus integer, 24 non-silent alleles were detected in an esterase locus (Wagner & Selander, 1974). This great variability of esterases was also found in bees (Frohlich et al, 1990;Bitondi & Mestriner, 1983), Anopheles darlingi (Santos et al, 1985), Anopheles triannulatus (Santos et al, 1992), Anopheles gambiae (Vernick et al, 1988), Paratheresia claripalpis (Marin & Mestriner, 1985), Aedes aegypti (Dinardo-Miranda & Contel, 1996), Spodoptera frugiperda (Lima & Contel, 1990), and other insects belonging to several orders (Prabhakaran & Kamble, 1994;Vaughan et al, 1995).…”

Section: Introductionmentioning

confidence: 91%

African dung beetle Onthophagus gazella Fabricius (Coleoptera: Scarabaeidae) esterase isozymes

Martins¹,

Contel²

2001

Braz. J. Biol.

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African beetles Onthophagus gazella from both sexes were analyzed by electrophoresis for an investigation of esterase isozymes using alpha-naphthyl propionate and methylumbelliferyl propionate as substrates. Only one of the esterases (Est. 6) reacted with one of the substrates (alpha-naphthyl propionate). Six areas of activity were found, two of them being polymorphic (Est. 3 and Est. 4). For presence of Est. 3, 337 individuals were analyzed, including descendants of 32 controlled crossings: two alleles were identified, whose frequencies are Est. 3A = 0.447 and Est. 3B = 0.553. The population is in equilibrium for this locus (qui-square = 4.18; 0.2 > P > 0.1). For Est. 4, 338 individuals, descendants of 32 controlled crossings, were analysed. In this case, three alleles were identified whose frequencies are: Est. 4A = 0.277; Est. 4B = 0.661; and Est. 4C = 0.062. The population is not in equilibrium for this locus (qui-square = 40.259; p < 0.001). Two esterases were detected only in the pupal stage and another one in larvae. Of the 23 loci analyzed in these insects up to now, 3 are polymorphic (13%), which indicates very low variability in the population here studied.

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BIOLOGIA DE ANOFELINOS AMAZÔNICOS. XIV. ISOENZIMAS DE ESTERASE EM ANOPHELES TRIANNULATUS (NEIVA & PINTO, 1922)

Cited by 5 publications

References 3 publications

Molecular divergence in the timeless and cpr genes among three sympatric cryptic species of the Anopheles triannulatus complex

Molecular divergence in the timeless and cpr genes among three sympatric cryptic species of the Anopheles triannulatus complex

Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

African dung beetle Onthophagus gazella Fabricius (Coleoptera: Scarabaeidae) esterase isozymes

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