Genética de populações de anofelinos IV. Estudos eletroforéticos das populações naturais de Anopheles nuneztovari e Unopheles darlingi. Correlação genética entre espécies

Narang, S. K.; Santos, Joselita Maria Mendes dos; Garcia, João C.; Cristakou, Helena D.; Narang, Neelam

doi:10.1590/1809-43921979093529

Cited by 6 publications

(5 citation statements)

References 1 publication

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“…The phenotypic variation de tected suggests that this locus has three alleles: ODH3*F, ODH3*S and ODH3*0. Similar data were observed by NARANG et al (1979a) in a popu lation of A. darlingi from BR-174 (Manaus/Boa Vista) and in a population of A. nuneztovari from Manaus. In both species, the authors reported three actvity zones.…”

Section: Octanol Dehydrogenasesupporting

confidence: 81%

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Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

1996

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-Of eleven proteins analyzed in four Amazonian populations, the esterases showed the greatest variation, with five activity zones. EST1, EST2 and EST5 showed variation in each of the populations studied. EST1 and EST2 are each controlled by two, and EST5 by four, codominant alleles. LAP presented six activity zones, with codominant variation in LAP5 and LAP6. oc-GPDH was monomorphic with one activity band on starch gel and two on polyacrylamide gel. 1DH presented two activity zones, with variation in the IDHl region. PGM had a single activity zone, with variation in all populations. The Ariquemes populations showed five alleles and the other populations three, all of then codominant. Three activity zones with two codominant alleles were observed for ODH. Aldehyde Oxidase showed two activity zones, with variation in AOl only in the Ariquemes and Porto Velho/Samuel populations. 6-PGDH showed only one activity zone and variation only in the Ariquemes population. The remaing systems -XDH, G-6-PDH and GDH. was monomorphic. Key words: Anopheles darlingi, isozymes, electrophoresisAnálise Eletroforética de 11 Enzimas era Populações Naturais de Anopheles (Ν.) darlingi Root, 1926 (Diptera: Cuiicidae) na Região Amazônica.RESUMO -Dos 11 sistemas protéicos analisados, em quatro populações da Amazônia, as esterases foram as que apresentaram maior variação, com cinco zonas de atividade. EST1, EST2, e EST5 mostraram variação genética determinada em todas populações estudadas, sendo que as duas primeiras são resultantes do controle de dois alelos e a EST5 de quatro, todos codominantes. A LAP consiste de seis zonas de atividade e com variação apenas nos loci LAP5 e LAP6, ambos codominantes. A GPDH mostrou-se monomórfica com uma zona de atividade em gel de amido e duas em poliacrilamida. A IDH apresentou duas zonas de atividade, com variação na região da IDH1. A PGM consiste de uma única zona de atividade e com variação em todas as populações estudadas. A população de Ariquemes revelou cinco alelos e as demais três, todos codominantes. Para a ODH, foram observadas três zonas de atividade com dois alelos codominantes. A enzima AO apresentou duas zonas de atividade, sendo que a AOl mostrou-se variável apenas em Ariquemes e Porto Velho/Samuel. 6-PGDH possui apenas uma zona de atividade e variação apenas em Ariquemes. Os demais sistemas -XDH, G-6-PDH e GDH -mostraram-se monomórficos.

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Section: Octanol Dehydrogenasesupporting

confidence: 81%

“…Phenotypic variants of IDH1 and IDH2 have been observed in the popu lations of A. nuneztovari from BR-174 (SCARPASSA, personal communica tion) and from Manaus for the IDH2 locus (NARANG et al 1979a). …”

Section: Isocitrate Dehydrogenasementioning

confidence: 99%

Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

1996

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“…Narang et al (1979) also found a high level of polymorphism (P = 0.632) for a population of A. darlingi from BR-174 (Manaus-Boa Vista). A relatively high degree of polymorphism has been also described in A. quadrimaculatus by Narang et al (1989) in 50% of the 33 loci analyzed.…”

Section: Levels Of Genetic Variabilitymentioning

confidence: 74%

“…In the case of null alleles for locus ODH1 of Porto Velho/Samuel population, it can be seen that the locus is in Hardy-Weinberg equilibrium. The occurrence of null alleles was observed in loci A and B of EST-C of A. punctipennis (Narang and Kitzmiller, 1971) and in the aldehyde oxidase of A. nuneztovari (Narang et al, 1979).…”

Section: Levels Of Genetic Variabilitymentioning

confidence: 96%

Intrapopulational genetic differentiation in Anopheles (N.) darlingi Root, 1926 (Diptera: Culicidae) in the amazon region

Santos¹,

Lobo

Tadei³

et al. 1999

Genet. Mol. Biol.

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Four natural Amazonian Anopheles darlingi populations were analyzed for 19 loci, 12 of which showed variation, i.e., 68.4% of polymorphic loci. The Ariquemes, Rondônia, population was the most variable, with a large number of alleles per locus (2.26 ± 0.27) and high polymorphism (P = 63.15). The highest values of observed and expected intralocus heterozygosity were observed in the Manaus, Amazonas, population (Ho = 0.432 ± 0.11; He = 0.375 ± 0.08), and the lowest in the Cachoeira Porteira, Pará, population (Ho = 0.236 ± 0.09; He = 0.290 ± 0.11). Wright's F-statistic revealed disequilibrium caused by an excess of homozygotes, as shown by the F is > F st values (F is = 0.083 > 0.026) reflecting intrapopulational differentiation. The four populations studied were genetically similar, as indicated by distance and similarity values. Chromosomal variation of Amazon population also did not indicate geographical differentiation, and populations in the central region of the Amazon Basin showed high polymorphism in relation to the marginal populations, which were mainly monomorphic.

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“…Variations in enzyme frequencies and chromosomes have been described. Narang et al (1979) and Steiner et al (1982) described isoenzyme variation in An. darlingi and other members of the subgenus Nyssorhynchus.…”

mentioning

confidence: 97%

Biological variation in Anopheles darlingi root

Charlwood

1996

Mem. Inst. Oswaldo Cruz

120

125

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Genética de populações de anofelinos IV. Estudos eletroforéticos das populações naturais de Anopheles nuneztovari e Unopheles darlingi. Correlação genética entre espécies

Cited by 6 publications

References 1 publication

Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

Intrapopulational genetic differentiation in Anopheles (N.) darlingi Root, 1926 (Diptera: Culicidae) in the amazon region

Biological variation in Anopheles darlingi root

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