Laser Photobiomodulation in the acute inflammatory response of the calcaneal tendon injury in rats exposed to cigarette smoke

Abstract

Nicotine delays the healing process and increases the levels of myeloperoxidase (MPO), an enzyme that plays a key role in the production of reactive oxygen species during the inflammatory process. Laser Photobiomodulation (PBM) is one of the most used electrophysical agents in the treatment of the calcaneal tendon, however, its effects on MPO activity need to be further elucidated. This study aimed to evaluate the effects of laser PBM on MPO activity after inflicting an injury to the calcaneal tendon of rats exposed to cigarette smoke. Thirty-four male Wistar rats with 90 days of age were used. After 14 days of exposure to cigarette smoke, the animals were divided into three experimental groups: control group (CG, n=12), not submitted to injury or treatment; sham group (ShG, n=10), submitted to partial calcaneal tendon injury and laser PBM simulation; and laser PBM group (PBMG, n=12), submitted to partial calcaneal tendon lesion and treated with laser PBM within the first minute after injury. PBM decreased MPO activity levels in PBMG compared to ShG (CG: 1.38±0.69pg/ml; ShG: 3.78±1.09pg/ml; PBMG: 2.58±0.93pg/ml; p<0.005). In conclusion, applying laser PBM immediately after inflicting damage to the calcaneal tendon attenuates acute inflammatory activity in rats exposed to cigarette smoke.

Efeitos da fotobiomodulação (laser de baixa intensidade) na cicatrização de feridas: revisão integrativa

“…Observou-se nessa revisão uma diferença na densidade de energia utilizada, bem como na frequência de utilização e na potência. Densidades de energia variaram de 1 a 20 J/cm 2 , sendo a mais utilizada a de 4J/cm 2 (Barbosa et al, 2020;Feitosa et al, 2015;Cunha et al, 2019;Taradaj et al 2018), 5J/cm 2 (Atasoy et al, 2017;Sousa et al, 2015;Cunha et al, 2019) (Nilforoushzadeh et al, 2019;Fioruzi et al, 2018;, 2J/cm 2 (Martins et al, 2015), 3J/cm 2 (Scherer et al 2019;De Souza et al, 2018), 0,25 J/cm 2 (Barbosa et al, 2020), 10 J/cm 2 e 20 J/cm 2 (Atasoy et al, 2017) e 8J/cm 2 ( Sousa et al, 2015). Observou-se, quanto aos parâmetros de aplicação da FBM, que eles variaram de 632,8 a 940 nm, mas a maior parte dos estudos (12= 75%) utilizou entre 632,8 a 670 nm, demonstrando uma padronização na utilização nas feridas.…”

Ultrabroad spectral response and excellent SERS performance of PbS-assisted Au/PbS/Au nanostars

“…[40][41][42] Besides, researchers also pay attention to the second harmonic generation (SHG) characteristics of noble metal nanomaterials, which could be widely used in laser devices, biology, and other fields. [43][44][45][46][47][48] In general, asymmetric metal nanostructures can produce strong SHG signals. Li et al reported that the SHG is caused by the anisotropy of the Au nanorods.…”