Caracterização Genética E Distribuição Geográfica Do Vírus Rábico Isolado De Bovinos No Estado De Mato Grosso, Brasil

Matta, Gustavo Corrêa; Nociti, Darci Lara Perecin; Carvalho, Adolorata Aparecida Bianco; Samara, Samir Issa; Ito, F. H.; Sakai, Takeo; Itou, Takuya; Sato, Go; Kobayashi, Yuki; Mochizuki, Nobuyuki

doi:10.1590/1808-1657v77p0192010

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…[19][20][21] The RABV genetic material is a single-stranded RNA molecule approximately 11.9-12.3 kb in length, and its viral genome is composed of five structural proteins: surface glycoprotein (G), nucleoprotein (N), phosphoprotein (P), matrix protein (M), and an RNA-dependent RNA polymerase (L). 21,22 The virion particles measure 60-110 nm × 130-250 nm and comprise a lipid envelope and a helical nucleocapsid. 21 Despite the severity of the disease, currently, available vaccines consist mainly of purified, concentrated, and inactivated rabies viruses, and are used in pre-and post-exposure protocols.…”

Section: Introductionmentioning

confidence: 99%

“…The Rabies virus (RABV) is a pathogen responsible for causing rabies, an anthropozoonotic disease with a mortality rate close to 100%, and is responsible for more than 60 000 deaths worldwide annually 19‐21 . The RABV genetic material is a single‐stranded RNA molecule approximately 11.9–12.3 kb in length, and its viral genome is composed of five structural proteins: surface glycoprotein (G), nucleoprotein (N), phosphoprotein (P), matrix protein (M), and an RNA‐dependent RNA polymerase (L) 21,22 . The virion particles measure 60–110 nm × 130–250 nm and comprise a lipid envelope and a helical nucleocapsid 21 .…”

Section: Introductionmentioning

confidence: 99%

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Monitoring by Raman spectroscopy of rabies virus‐like particles production since the initial development stages

Guardalini,

Rangel,

Leme

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUNDThis study aimed to establish chemometric models using Raman spectroscopy data for biochemical monitoring of rabies Virus‐Like Particles (VLP) production based on baculovirus/insect cell system. The models were developed using fresh and stored samples from the initial development stages (Schott culture flasks). The following modeling techniques were assessed: partial least squares (PLS) and artificial neural networks (ANN). The effects of spectral filtering approaches, spectral ranges (400–1850 cm−1; 100–3425 cm−1), and sample cryopreservation were also considered. The applicability of the models was evaluated using experimental data from assays carried out in a benchtop bioreactor.RESULTSThe results showed that the prediction capacity of the chemometric models was negatively impacted when samples from rabies VLP production were cryopreserved. Further studies are needed to confirm the maximum storage time for samples (< 4 months) without a significant difference in model predictions compared to those from an in line database. The dilution of the sample should be kept constant throughout the rabies VLP development stages. A nonlinear correlation was observed between dilution and the predicted values of biochemical parameters from Raman spectral data. The choice of spectral filtering has a major impact on the prediction accuracy of chemometric models.CONCLUSIONThe optimal filtering approach should be individually optimized for each biochemical parameter. The ANN models were significantly more suitable for biochemical monitoring than the PLS approach. The 400–1850 cm−1 Raman shift range is recommended for biochemical monitoring of rabies VLP using a baculovirus/insect cell platform when samples are cell‐free. © 2023 Society of Chemical Industry (SCI).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Monitoring by Raman spectroscopy of rabies virus‐like particles production since the initial development stages

Guardalini,

Rangel,

Leme

et al. 2023

J of Chemical Tech & Biotech

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Genotyping of rabies positive samples isolated from animals in Mato Grosso and Rondônia – Brazil

Pimentel

Nassarden²,

Cândido

et al. 2022

Infection, Genetics and Evolution

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Distribuição Espaço-Temporal Dos Diagnósticos Laboratoriais Da Raiva Animal

et al. 2015

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Resumo:A raiva é uma enfermidade infectocontagiosa que afeta mamíferos, inclusive o homem. Estima-se que a raiva dos herbívoros seja responsável por enormes prejuízos econômicos na América Latina. O morcego hematófago Desmodus rotundus é o principal transmissor do vírus rábico (VR). Este estudo teve como objetivo avaliar a evolução do diagnóstico laboratorial para raiva animal em cinco espécies animais, na série cronológica 1990-2010, na microrregião Quirinópolis, Goiás, Brasil. Para avaliação da distribuição dos casos diagnosticados foram utilizados resultados de diagnóstico laboratorial, realizados pelo Laboratório de Diagnóstico da Agência Goiana de Defesa Agropecuária. A partir destes, foi construído um banco de dados com as variáveis: município de origem do animal, resultado, mês e ano. Das 116 amostras analisadas, 9% (10) foram positivas para oVR e 91% (106) negativas. Todos os resultados positivos foram provenientes de bovinos. Dentre os municípios da microrregião, Itarumã apresentou maior número de casos positivos e Caçu enviou mais amostras. Houve tendência de aumento no envio de amostras e no número de exames laboratoriais. Também houve tendência ao aumento de diagnósticos positivos. Amostras da espécie bovina foram as mais analisadas, seguida de morcegos hematófagos. Em relação à época do ano, foram observados mais casos positivos no período da seca. Palavras-chave: Animais domésticos; epidemiologia; quirópteros; vírus rábico. Abstract:Rabies is an infectious contagious disease that affects mammals, including humans. Herbivore rabies is estimated to be the cause of major economic downturns in Latin America. The hematophagous bat Desmodusrotundus is the main transmitter of the rabies virus. The present study aimed at evaluating the laboratory diagnosis of animal rabies in five species of animals, in the 1990-

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Caracterização Genética E Distribuição Geográfica Do Vírus Rábico Isolado De Bovinos No Estado De Mato Grosso, Brasil

Cited by 3 publications

References 17 publications

Monitoring by Raman spectroscopy of rabies virus‐like particles production since the initial development stages

Monitoring by Raman spectroscopy of rabies virus‐like particles production since the initial development stages

Genotyping of rabies positive samples isolated from animals in Mato Grosso and Rondônia – Brazil

Distribuição Espaço-Temporal Dos Diagnósticos Laboratoriais Da Raiva Animal

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