Bioprospecting Aspergillus section Nigri in Atlantic Forest soil and plant litter

Abstract

ABSTRACT:The use of fungi as a source of enzymes has become widespread in various industrial and commercial areas, and Aspergillus section Nigri has significant potential for producing enzymes. The aim of this study was to isolate Aspergillus section Nigri from plant litter and soil from the Atlantic Forest biome and evaluate it with regards to hydrolytic enzyme production. The trials for producing the enzymes were carried out in Petri dishes, using different culture mediums adapted for microbial growth and with the respective substrates for inducing enzyme production -cellulase (carboxymethyl cellulose), protease (skimmed milk), amylase (soluble starch), pectinase (citrus pectin), and phytase (Pikovskaya medium). Forty-two fungi were isolated, 16.7% derived from the plant litter layer and 83.3% derived from soil at a depth of 0 to 5 cm and 5 to 10 cm. All of the isolated lineages presented amylase, protease, and phytase production, with 90.4% producing cellulase and no lineage producing pectinase. From the results, the significant potential for Atlantic Forest fungi as hydrolytic enzyme producers could be perceived. The enzymatic activity evaluations presented a satisfactory result when compared with the scientific literature.

Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability

“…Since CMC can be degraded by other enzymes in addition to EG, talking about CMCase activity is correct. In this sense, we can say that A. niger LBM 134 has positive CMCase activity which coincides with the literature (Castrillo et al 2012;Martínez et al 2005;Panda et al 2012;Singhania et al 2017;Maia and Fraga 2017). Respect on BGL and CBH activities, the fluorescence was intense after a few days of incubation; data agreed with the literature that describes species of A. niger as BGLs and CBHs producer and the technique of fluorescence plate contributed more significant results in the study of the potential cellulolytic of A. niger LBM 134.…”

Fauna Ameaçada Nas Rodovias

“…Estes requisitos incluem: baixa toxicidade, não patogênico e seguro para saúde humana. As espécies Aspergillus oryzae e Aspergillus niger já possuem o status de GRAS e, portanto tem sido comercialmente explorados(MAIA & FRAGA, 2017).…”