“…Compared to the staining and clearing method, one study reported that CBCT is as accurate as staining and clearing in studying root and canal anatomy in several tooth types (Neelakantan et al, 2010b); others found that both methods were not able to detect complex canal anatomy (Ordinola‐Zapata et al, 2017), and CBCT was not able to detect accessory canals (Naseri et al, 2019). A number of studies have compared the accuracy of CBCT with micro‐CT; the results revealed that, despite the higher accuracy compared with 2D radiographic imaging, CBCT was less accurate than micro‐CT in detecting complex canal anatomy (Sousa et al, 2017; Zhang et al, 2017a), single oval canals (Paes da Silva Ramos Fernandes et al, 2014), apical canal isthmuses (Tolentino et al, 2018) and apical deltas (Nascimento et al, 2019), consistent with results of a recent systematic review (Borges et al, 2020) (Figure 15). Apart from comparative studies with other diagnostic methods, CBCT has also been used as the diagnostic tool for the study of root canal anatomy including root canal configurations, merging and splitting levels of canals and other landmarks related to the shape of root canal orifices obtained from axial sections (Jain et al, 2017; Neelakantan et al, 2010a; Saber et al, 2019).…”