Additive manufacturing of titanium alloy could modify the pathogenic microbial profile: an in vitro study

Pingueiro, João Marcos Spessoto; Piattelli, Adriano; Paiva, Juliana; Figueiredo, Luciene Cristina; Feres, Magda; Shibli, Jamil Awad; Bueno‐Silva, Bruno

doi:10.1590/1807-3107bor-2019.vol33.0065

Cited by 21 publications

(22 citation statements)

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“…The measurements of the experimental groups were compared against those of the negative and positive controls. Counts below the method detection limit (1 × 10 4 ) were considered zero [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

et al. 2020

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This study investigated the effects of Brazilian Red Propolis (BRP) extract on seven-day-old multispecies subgingival biofilms. Mixed biofilm cultures containing 31 species associated with periodontal health or disease were grown for six days on a Calgary device. Then, mature biofilms were treated for 24 h with BRP extract at different concentrations (200–1600 µg/mL), amoxicillin (AMOXI) at 54 µg/mL (positive control) or vehicle (negative control). Biofilm metabolic activity was determined by colorimetry, and bacterial counts/proportions were determined by DNA–DNA hybridization. Data were analyzed by Kruskal–Wallis and Dunn’s tests. Treatment with BRP at 1600, 800 and 400 μg/mL reduced biofilm metabolic activity by 56%, 56% and 57%, respectively, as compared to 65% reduction obtained with AMOXI. Mean total cell counts were significantly reduced in all test groups (~50–55%). Lower proportions of red, green and yellow complex species were observed upon treatment with BRP (400 µg/mL) and AMOXI, but only AMOXI reduced the proportions of Actinomyces species. In conclusion, BRP extract was as effective as AMOXI in killing seven-day-old multispecies biofilm pathogens and did not affect the levels of the host-compatible Actinomyces species. These data suggest that BRP may be an alternative to AMOXI as an adjunct in periodontal therapy. In vivo studies are needed to validate these results.

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Section: Methodsmentioning

confidence: 99%

Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

et al. 2020

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“…In this line, classic studies have demonstrated increased biofilm formation on transmucosal implant surfaces with higher surface roughness and surface free energy [ 37 , 38 ]. As the long-term survival of dental implants partially depends on the control of bacterial infection in the peri-implant region [ 12 , 39 , 40 ], long-term clinical studies are necessary for drawing more profound conclusions on whether or not rough transmucosal surfaces might have a detrimental effect on the peri-implant microbial parameters and soft and hard supporting tissues stability.…”

Section: Discussionmentioning

confidence: 99%

The Potential Role of a Surface-Modified Additive-Manufactured Healing Abutment on the Expression of Integrins α2, β1, αv, and β6 in the Peri-Implant Mucosa: A Preliminary Human Study

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et al. 2022

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The stability of peri-implant soft tissues is essential for long-term success. Integrins play a vital role in biological processes through developing and maintaining cell interactions; however, few studies have evaluated the effects of modifications to abutment surfaces on cell adhesion across integrin expression. Therefore, this pilot study assessed the influence of different surface topographies of titanium healing abutments prepared by additive manufacturing (AM) on the gene expression levels of the integrin subunits α2, β1, αv, and β6 in the human peri-implant mucosa. Thirteen healthy adults were included. Depending on the number of required implants, the subjects were distributed in different groups as a function of healing abutment topography: group 1 (fully rough surface); group 2 (upper machined + lower rough); group 3 (rough upper surface + lower machined); group 4 (fully machined). A total of 40 samples (n = 10/group) of the peri-implant mucosa around the abutments were collected 30 days after implant placement, and subsequently, the gene expression levels were evaluated using real-time PCR. The levels of gene expression of β1-subunit integrin were upregulated for individuals receiving fully rough surface abutments compared with the other surface topographies (p < 0.05). However, the healing abutment topography did not affect the gene expression levels of the α2, αv, and β6 integrin subunits in the human peri-implant mucosa (p > 0.05). This preliminary study suggested that controlled modifications of the surface topography of titanium healing abutments produced by AM may influence the quality of the peri-implant mucosa in the early stages of the soft tissue healing process.

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“…It was proved, that the DLMS process associated with chemical treatment modifies the in vitro biofilm profile. What is more, this process reduced the proportion of red complex and decreased the total counts of Porphyromonas gingivalis and it also favored (to a lesser extent) Parvimonas micra , which might be related to peri-implant disease [ 113 ]. The point is that peri-implantitis is a ubiquitous disease and is directly influenced by the passage of time and surely, the more time passes, the more implants will be affected by peri-implantitis.…”

Section: Discussionmentioning

confidence: 99%

Methods of Topical Administration of Drugs and Biological Active Substances for Dental Implants—A Narrative Review

et al. 2021

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Dental implants are, nowadays, established surgical devices for the restoration of lost teeth. Considered as an alternative for traditional prosthetic appliances, dental implants surpass them in reliability and patient feedback. Local drug delivery around the implants promotes osseointegration and reduces peri-implantitis. However, there are currently no methods of a multiple, precise topical administration of drugs to the implant area. Engineering coatings on the implants, drug application on carriers during implantation, or gingival pockets do not meet all requirements of dental surgeons. Therefore, there is a need to create porous implants and other medical devices that will allow a multiple drug delivery at a controlled dose and release profile without traumatic treatment. Due to the growing demand for the use of biologically active agents to support dental implant treatment at its various stages (implant placement, long-term use of dental superstructures, treatment of the peri-implant conditions) and due to the proven effectiveness of the topical application of pharmacological biologically active agents to the implant area, the authors would like to present a review and show the methods and devices that can be used by clinicians for local drug administration to facilitate dental implant treatment. Our review concludes that there is a need for research in the field of inventions such as new medical devices or implants with gradient solid–porous structures. These devices, in the future, will enable to perform repeatable, controllable, atraumatic, and repeatable injections of active factors that may affect the improvement of osteointegration and the longer survival of implants, as well as the treatment of peri-implantitis.

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Additive manufacturing of titanium alloy could modify the pathogenic microbial profile: an in vitro study

Abstract: Juliana PAIVA (a) Luciene Cristina de FIGUEIREDO (a) Magda FERES (a) Jamil SHIBLI (a) Bruno BUENO-SILVA (a)

Cited by 21 publications

References 24 publications

Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

The Potential Role of a Surface-Modified Additive-Manufactured Healing Abutment on the Expression of Integrins α2, β1, αv, and β6 in the Peri-Implant Mucosa: A Preliminary Human Study

Methods of Topical Administration of Drugs and Biological Active Substances for Dental Implants—A Narrative Review

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