Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine®

Cosme‐Silva, Leopoldo; Dal‐Fabbro, Renan; Gonçalves, Luanna de Oliveira; Prado, Alana Sant'Ana do; Plazza, Flávia Alfredo; Viola, Naiana Viana; Cintra, Luciano Tavares Ângelo; Gomes‐Filho, João Eduardo

doi:10.1590/1807-3107bor-2019.vol33.0060

Cited by 16 publications

(18 citation statements)

References 32 publications

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“…MTA Repair HP demonstrates proper cytological compatibility with human pulp stem cells (hDPSC). Previously, it was demonstrated that MTA Repair HP can promote a biological response in hDPSC in terms of cell proliferation, morphology, migration and binding, while the material is still cytocompatible [33].…”

Section: Discussionmentioning

confidence: 99%

“…Differentiation of monocytes THP-1 to macrophages THP-1 (activation of monocytic cells THP-1) was measured by flow cytometry. The culture model in vitro used in the present study has been previously used for the purpose of clinical verification of the induction of inflammatory reaction of materials based on hydrated calcium silicate with odontotropic properties [33].The control cells were incubated in 10% FBS RPMI medium as control 24 (C 24) for 24 h for 48 h as control 48 (C 48).…”

Section: Mta Repair Hp-induced Inflammatory Reaction In Thp-1 Monocytesmentioning

confidence: 99%

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The Influence of New Silicate Cement Mineral Trioxide Aggregate (MTA Repair HP) on Metalloproteinase MMP-2 and MMP-9 Expression in Cultured THP-1 Macrophages

Barczak

Palczewska-Komsa

Lipski

et al. 2020

IJMS

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The aim of the present study was to investigate the new silicate cement mineral trioxide aggregate (MTA Repair HP) with respect to its effect on the inflammation process involving the tooth and periodontal tissues. The composition of MTA Repair HP was supplemented with plasticizer agents which can have a negative effect on the modulation of tooth inflammation. The silicate-based material in question is widely used in regeneration of the pulp-dentin complex, treatment of perforations of various locations in the tooth, as well as in surgical treatment of the complications of periapical tissue. The improved bioceramic restorative cement can affect the expression of metalloproteinases MMP-2 and MMP-9 in monocytes/macrophages involved in modulation of inflammation and regenerative processes of the tooth and periodontal tissues. The novel aspect of the present study lies in the application of the model of THP-1 monocyte/macrophage and applying the biomaterial in direct contact with the cells. Hence, it provides a representation of clinical conditions with respect to regenerative pulp and periodontal treatment with the use of MTA Repair HP. A lack of macrophage activation (as measured with flow cytometry) was found. Moreover, the study identified a lack of expression stimulation of the studied metalloproteinases (with the use of Western blotting and fluorescent microscopy). Similarly, no increase in MMP-2 and MMP-9 concentration was found (measured by ELISA method) in vitro when incubated with MTA Repair HP. Based on the results it can be concluded that new MTA Repair HP does not increase the inflammatory response in monocytes/macrophages associated with the activity of the described enzymes. It can also be speculated that they do not affect the process of dentin regeneration in which MMP-2 and MMP-9 play significant roles.

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Section: Discussionmentioning

confidence: 99%

Section: Mta Repair Hp-induced Inflammatory Reaction In Thp-1 Monocytesmentioning

confidence: 99%

The Influence of New Silicate Cement Mineral Trioxide Aggregate (MTA Repair HP) on Metalloproteinase MMP-2 and MMP-9 Expression in Cultured THP-1 Macrophages

Barczak

Palczewska-Komsa

Lipski

et al. 2020

IJMS

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“…The group of research papers on the biological properties comprised a total of 18 scientific articles, including: seven studies on human cells in vitro [ 12 , 23 , 24 , 25 , 26 , 27 , 28 ], seven on Wistar rats in vivo [ 6 , 9 , 18 , 19 , 20 , 21 , 22 ], two research papers on mice in vitro [ 21 , 29 ] and three papers cells in vitro [ 37 , 38 , 39 ]. A brief description of the aforementioned studies is presented in Table 7 .…”

Section: Resultsmentioning

confidence: 99%

“…Out of the biological parameters, the inflammatory response of the tissues in contact with MTA HP is discussed particularly often [ 6 , 9 , 12 , 18 , 20 , 21 ]. From the clinical perspective, the most desirable situation is that of a lack of such a response, which would indicate an ideal biocompatibility of the material covering the tissue.…”

Section: Discussionmentioning

confidence: 99%

New Bioactive Calcium Silicate Cement Mineral Trioxide Aggregate Repair High Plasticity (MTA HP)—A Systematic Review

2021

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Bioactive calcium silicate cement Mineral Trioxide Aggregate (MTA) has been used for years as a gold standard in intravital pulp treatment and specialist endodontic procedures. Owing to flaws of the material, the manufacturers have been trying to enhance and produce materials showing improved physical, chemical and biological parameters. One of the new calcium-silicate cements based on mineral trioxide aggregate, however without some flaws exhibited by the cement, is Mineral Trioxide Aggregate Repair High Plasticity (MTA HP). The aim of the present paper was a systematic literature review concerning the MTA HP material used nowadays in dentistry, as a review of its specific features. The present paper is the first article providing a systematic literature review on MTA HP. The aim of the present article is the better understanding of MTA HP properties, which can aid the decision-making process in endodontic treatment.

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“…É importante ressaltar que a condição sistêmica pode influenciar na resposta tecidual frente aos materiais. Cosme-Silva et al (2019) demonstraram através de uma pesquisa em subcutâneo de ratos hipertensos, que a a condição sistêmica de hipertensão pode aumentar a resposta inflamatória e reduzir a biomineralização de materiais como MTA e Biodentine ® . Tal achado evidencia a importância de considerar a saúde do paciente para cada caso individualmente e que condições sistêmicas influenciam o tratamento dentário, não devendo de forma alguma ser considerado à parte.…”

Section: Discussionunclassified

Avaliação da biocompatibilidade de cimentos reparadores biocerâmicos: Estudo in vivo em ratos wistar

Valentim

Bueno

Marques

et al. 2021

RSD

Self Cite

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O objetivo deste estudo foi avaliar a biocompatibilidade do cimento biocerâmico reparador Biodentine® quando comparado ao MTA Branco Angelus® e hidróxido de cálcio. Para isso foram utilizados vinte e quatro ratos Wistar (n=6 animais/grupo), divididos em quatro tempos experimentais de 7, 15, 30 e 60 dias. Os animais receberam implantes subcutâneos de tubos de polietileno contendo os 3 materiais e um tubo vazio utilizado como controle. Após os períodos experimentais os animais foram eutanasiados e os tubos, juntamente com o tecido circundante, foram removidos e processados histologicamente para avalição da biocompatibilidade. Infiltrado inflamatório e espessura de cápsula fibrosa foram analisados por coloração de HE através de escores de inflamação. Os dados foram submetidos ao teste de Kruskal Wallis e Dunn (p<0,05). O Biodentine® aos 15 dias gerou menor resposta inflamatória que o controle e o Ca(OH)2 (p<0,05). No grupo hidróxido de cálcio, apartir dos 15 dias em diante houve diminuição do infiltrado inflamatório, assim como diminuição da cápsula fibrosa. O MTA Branco Angelus® mostrou capsula fibrosa fina e baixa intensidade inflamatório em todos os períodos. Após os períodos experimentais, todos materiais apresentaram inflamação leve e capsula fibrosa fina. Conclui-se que o Biodentine® demonstrou biocompatibilidade tecidual pois induziu baixa inflamação, que reduziu ao longo do tempo, comparável ao MTA Angelus Branco® e hidróxido de cálcio.

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Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine®

Abstract: Renan DAL-FABBRO (a) Luanna de Oliveira GONÇALVES (a) Alana Sant'Ana do PRADO (a) Flávia Alfredo PLAZZA (a) Naiana Viana VIOLA (b) Luciano Tavares Angelo CINTRA (a) João Eduardo GOMES FILHO (a)

Cited by 16 publications

References 32 publications

The Influence of New Silicate Cement Mineral Trioxide Aggregate (MTA Repair HP) on Metalloproteinase MMP-2 and MMP-9 Expression in Cultured THP-1 Macrophages

The Influence of New Silicate Cement Mineral Trioxide Aggregate (MTA Repair HP) on Metalloproteinase MMP-2 and MMP-9 Expression in Cultured THP-1 Macrophages

New Bioactive Calcium Silicate Cement Mineral Trioxide Aggregate Repair High Plasticity (MTA HP)—A Systematic Review

Avaliação da biocompatibilidade de cimentos reparadores biocerâmicos: Estudo in vivo em ratos wistar

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