Anadenanthera Colubrina vell Brenan: anti-Candida and antibiofilm activities, toxicity and therapeutical action

Silva, Diego Romário; Rosalen, Pedro Luiz; Freires, Irlan Almeida; Sardi, Janaína de Cássia Orlandi; Lima, Rennaly de Freitas; Lazarini, Josy Goldoni; Costa, Tereza Karla Vieira Lopes da; Pereira, Jozinete Vieira; Godoy, Gustavo Pina; Costa, Edja Maria Melo de Brito

doi:10.1590/1807-3107bor-2019.vol33.0023

Cited by 20 publications

(15 citation statements)

References 33 publications

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“…A. colubrina extract maintained the cell viability higher than 70% at concentrations up to 250 μg/ ml, representing a relatively low cytotoxic activity. This finding is consistent with results recently reported, which verified the low toxicity of the bark extract of A. colubrina on human cells lines, such macrophages (Silva et al, 2019;Cardoso-Junior et al, 2020), keratinocytes and also on tumoral cell strains (Lima et al, 2014). These data corroborate to the results found in this study related to the low toxic potential effect of this product, shown to be pharmacological safe in vitro, by maintaining the cell viability.…”

Section: Discussionsupporting

confidence: 94%

Yeast-Host Interactions: Anadenanthera colubrina Modulates Virulence Factors of C. albicans and Inflammatory Response In Vitro

et al. 2021

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Oral candidiasis is one of the most common fungal infections in humans. Its incidence has increased widely, as well as the antifungal resistance, demanding for the search for novel antifungal therapeutic agents. Anadenanthera colubrina (Vell.) Brenan is a plant species that has been proven to possess pharmacological effects, including antifungal and anti-inflammatory activities. This study evaluated in vitro the effects of standardized A. colubrina extract on virulence factors of Candida albicans and its regulation on immune response through C. albicans-host interaction. Antifungal activity was evaluated by Broth Microdilution Method against reference Candida strains (C. albicans, C. glabrata, C. tropicalis; C. dubliniensis). Anti-biofilm effect was performed on C. albicans mature biofilm and quantified by CFU/mL/g of biofilm dry weight. Proleotlytic enzymatic activities of proteinase and phospholipase were assessed by Azocasein and Phosphatidylcholine assays, respectively. Cytotoxicity effect was determined by Cell Titer Blue Viability Assay on Human Gingival Fibroblasts. Co-cultured model was used to analyze C. albicans coexisting with HGF by Scanning Electron Microscopy and fluorescence microscopies; gene expression was assessed by RT-PCR of C. albicans enzymes (SAP-1, PLB-1) and of host inflammatory cytokines (IL-6, IL-8, IL-1β, IL-10). Cytokines secretion was analysed by Luminex. The extract presented antifungal effect with MIC<15.62 μg/ml against Candida strains. Biofilm and proteolytic activity were significant reduced at 312.4 μg/ml (20 × 15.62 μg/ml) extract concentration. Cell viability was maintained higher than 70% in concentrations up to 250 μg/ml (LD50 = 423.3 μg/ml). Co-culture microscopies demonstrated a substantial decreased in C. albicans growth and minimal toxicity against host cells. Gene expressions of SAP-1/PLB-1 were significantly down-regulated and host immune response was modulated by a significant decreased on IL-6 and IL-8 cytokines secretion. A. colubrina had antifungal activity on Candida strains, antibiofilm, and anti-proteolytic enzyme effects against C. albicans. Presented low cytotoxicity to the host cells and modulatory effects on the host immune response.

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Section: Discussionsupporting

confidence: 94%

Yeast-Host Interactions: Anadenanthera colubrina Modulates Virulence Factors of C. albicans and Inflammatory Response In Vitro

et al. 2021

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“…Probiotics participate in the immune response modulation by increasing the macrophage activation [7] and altering the release of proinflammatory and anti-inflammatory cytokines [8][9][10][11]. Thus, the use of probiotics is considered a promising strategy for the prevention and the control of various infectious diseases [12].…”

Section: Introductionmentioning

confidence: 99%

Bacillus amyloliquefaciens-9 as an Alternative Approach to Cure Diarrhea in Saanen Kids

Zhang

Xin

Jiang

et al. 2021

Animals

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Bacillus amyloliquefaciens-9 (GBacillus-9), derived from the intestinal tract of the white-spotted bamboo shark, secretes a variety of antimicrobial compounds that inhibit the growth of pathogenic bacteria. In this study, the role of GBacillus-9 in the prevention and treatment of Saanen kids with diarrhea was assessed. Six healthy kids (HL) and six kids with diarrhea (DL) were selected. All kids were fed with 0.3% (w/v) GBacillus-9 (spray power) in raw milk for two weeks. The proportion of kids with diarrhea decreased gradually as the trial progressed, and 100% DL kids were cured at day 15. GBacillus-9 increased the serum immunoglobulin (Ig) G, interleukin (IL)-4, and IL-6 concentration (p < 0.05). The amplicon sequencing analysis of the fecal bacterial community revealed that the fecal microbiota was remarkably different between the HL and the DL groups at day 0. After two weeks of feeding with GBacillus-9, no significant difference in fecal microbiota was observed between HL and DL groups at the phylum level. GBacillus-9 restored the intestinal microbial disorder associated with serum immunoglobulin and interleukin concentration. Correlation analysis showed that GBacillus-9 altered globulin and interleukin concentration and that immunoglobulin was associated with Firmicutes. Collectively, our results revealed that GBacillus-9 improved the gut health of kids by improving microbial homeostasis

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“…Thus, the search for new alternatives in antibacterial therapy is required, with a special interest in natural remedies as reported by Silva et al, (2019). In this context, de Melo Pereira et al, (2018)…”

Section: Discussionmentioning

confidence: 99%

In vitro inhibition of uropathogenic Escherichia coli biofilm formation by probiotic Lactobacilli isolated from healthy breast fed infants

Hashem

El-Baky

2021

Novel Research in Microbiology Journal

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Biofilm forming Escherichia coli bacterium exhibits multiple drug resistance, which is responsible for recurrent urinary tract infections (UTI) that are difficult to eradicate. The work aimed to investigate the antibacterial and antibiofilm activities of Lactobacilli isolated from faecal microbiota of healthy infants; to identify these isolates and determine their probiotic characteristics. E. coli isolates were recovered from urine samples of patients with urinary tract infections. On the other hand, Lactobacillus isolates were recovered from faeces of breast-fed infants. Five strong biofilm forming E. coli isolates with multidrug resistance (MDR) were selected. The antibacterial potential of Lactobacillus supernatants were assessed via disk diffusion assay. All the tested E. coli isolates showed high susceptibility to the Lactobacillus supernatants; where 54 % of these supernatants expressed inhibition zones diameters ranging from 15-18 mm. Antibiofilm efficacies of Lactobacillus spp. against E. coli isolates were tested in vitro using microtiter plate assay and scanning electron microscopy (SEM). More than 50 % reduction of biofilms formation by the 5 selected MDR E. coli isolates was observed by most the Lactobacillus isolates. The scanning electron microscopy confirmed the elimination of E. coli biofilms by cells of the Lactobacillus isolates. Preliminary probiotic characteristics of the Lactobacillus isolates were investigated; all isolates tolerated 2 % bile salt concentration and acidic condition at pH 3. Regarding safety of the Lactobacilli for human consumption, all isolates were non hemolytic, and 14 Lactobacillus isolates were sensitive to all tested antibiotics except for vancomycin, as they are naturally resistant to it. About 14 safe probiotic Lactobacillus isolates were identified by API-50 CHL test as; Lactobacillus acidophilus, L. plantarum, L. fermentum and L. paracasei.

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Anadenanthera Colubrina vell Brenan: anti-Candida and antibiofilm activities, toxicity and therapeutical action

Cited by 20 publications

References 33 publications

Yeast-Host Interactions: Anadenanthera colubrina Modulates Virulence Factors of C. albicans and Inflammatory Response In Vitro

Yeast-Host Interactions: Anadenanthera colubrina Modulates Virulence Factors of C. albicans and Inflammatory Response In Vitro

Bacillus amyloliquefaciens-9 as an Alternative Approach to Cure Diarrhea in Saanen Kids

In vitro inhibition of uropathogenic Escherichia coli biofilm formation by probiotic Lactobacilli isolated from healthy breast fed infants

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