The effects of smoking on the expression of gelatinases in chronic periodontitis: a cross-sectional study

Abstract

Smokers have a risk of developing periodontal disease. Matrix metalloproteinases (MMP) play a significant role in periodontal tissue destruction. In this study possible relationship between smoking and gingival tissue expression of gelatinases in chronic periodontitis patients relative to periodontally healthy subjects was investigated. Forty chronic periodontitis patients (20 smokers and 20 non-smokers) and forty periodontally healthy subjects (20 smokers and 20 non-smokers) were enrolled. The clinical periodontal measurements recorded, and gingival tissues harvested after that. After histologic evaluation, matrix metalloproteinases-2 and-9 expressions were analyzed immunohistochemically. In nonsmokers, higher expression of metalloproteinases-2 and-9 detected in chronic periodontitis group compared to the periodontally healthy group. In the smoker chronic periodontitis group, the expression of metalloproteinases-2 was lower than nonsmoker chronic periodontitis group. Statistically significant differences detected between smoker and nonsmoker periodontally healthy groups in metalloproteinases-2 expression. For metalloproteinases-9 expression, smoker chronic periodontitis group has lower values than nonsmoker chronic periodontitis group. In periodontally healthy group smokers showed higher metalloproteinases-9 expressions than non-smokers. Present findings support the role of gelatinases in chronic periodontitis pathogenesis. Based on the current results we conclude that smoking alters the expression of gelatinases in gingival tissues.

The Effect of Non-surgical Periodontal Therapy on Periodontal Parameters, Malondialdehyde, Total Oxidant Status And Antioxidant Capacity in Smokers with Chronic Periodontitis

“…Regarding BOP, the present result was in agreement with a study reported an overall tendency to more bleeding sites in the NSP with no statistically significant differences as compared to SP (Luzzi et al, 2007). In contrast, another studies reported that SP patients exhibited statistically significant decrease of BOP than did in NSP (Machuca et al, 2000;Bergström and Boström, 2001;Sreedeviet al, 2012;Şentürk et al, 2018).…”

The relationship between periodontal status and rheumatoid arthritis – systematic review

“…In 2018, results of studies on the effect of smoking on MMP-2 and MMP-9 levels in patients with PD showed lower expression of MMP-2 and MMP-9 in the saliva, but increased serum concentration in smokers compared to non-smokers, which proves a different effect of nicotine on tissues [ 48 ].…”

Evaluation of the papillary gingival vasculature in smokers and nonsmokers with chronic periodontitis: A clinical in vivo study

“…Smoking has been clearly established as one of the most important risk factors in the development and progression of periodontal disease. [ 1 2 3 ] It has been associated with periodontal tissue destruction,[ 4 5 6 7 8 9 ] impaired wound healing following various surgical procedures,[ 10 11 ] negative outcome of periodontal therapy,[ 12 13 14 15 16 17 ] and impairment of gingival vasculature. [ 18 19 ]…”