Effect of acute administration of nicotine and ethanol on tooth movement in rats

Abstract

All the solutions were applied for 32, 44, or 58 days, according to the subgroup. Orthodontic movement (25 cN) was initiated 30 days after solution administration in the groups with tooth movement. The rats were euthanized 2, 14, or 28 days after initiation of tooth movement. Tooth sections were stained using picrosirius and tartrate-resistant acid phosphatase (TRAP). The data were compared by ANOVA using Tukey's HSD and Games-Howell. On day 28 of tooth movement, the NEM group had a lower percentage of type I collagen compared to the SM group (p = 0.0448), and the S group had a higher number of osteoclasts/μm 2 compared to the N group (p = 0.0405). Nicotine and ethanol did not affect the tooth movement rate, regardless of induction of orthodontic movement. Nicotine influenced the number of osteoclasts by decreasing their quantity when dental movement was not induced. When nicotine was associated with ethanol, it interfered in the maturation of collagen fibers during orthodontic movement.

Effect of nicotine on orthodontic tooth movement and bone remodeling in rats

“…For the in vivo study, they only used one dose of nicotine 1.89 mg/kg and a small sample of 14 male rats. Araujo et al 28 reported that nicotine decreased the number of osteoclasts when dental movement was not induced (nicotine 1 mg/kg without tooth movement group). They calculated the mean number of osteoclasts per square micrometer of all periodontal ligaments in order to avoid the risk of bias related to selection of the compression side of the periodontal ligament and alveolar bone.…”

“…They calculated the mean number of osteoclasts per square micrometer of all periodontal ligaments in order to avoid the risk of bias related to selection of the compression side of the periodontal ligament and alveolar bone. 28 Gonzales et al 18 measured M1-M2 using digitized lateral cephalograms and observed the largest and deepest root resorption craters on the disto-buccal root which appeared to the compression zone, using 3D images obtained via a laser scanning electron microscope. In the present study, osteoclasts were counted on the mesial alveolar bone surface of the distobuccal root of M1, which was defined as the compression side of OTM.…”

The influence of fluoxetine on orthodontic tooth movement in rats

“…An orthodontic device was installed, 20 consisting of a closed-spring nickel-titanium (G&H Wire, Franklin, IN) attached to the first upper right molar with a 0.010" stainless steel ligature wire (Morelli, São Paulo, Brazil) and connected to the upper central incisors, causing the mesial movement of the molar (25 cN), 21 which was standardized with a tensiometer (Haag-Streit AG, Koeniz, Switzerland) (Figure1). 22 To ensure greater spring stability, the lower incisors were worn down and the upper incisors bonded together with the Charisma composite resin (Heraeus, Hanau, Germany). After installation, the spring was not reactivated.…”

Pharmaka, Noxen und die Ernährung – welche anamnestische Relevanz haben sie für die kieferorthopädische Therapie?

“…Diese Substanzen können pharmakologisch im Organismus wirksam werden und somit unbeabsichtigt die kieferorthopädische Zahnbewegung, die Entstehung von Zahnwurzelresorptionen und das parodontale Attachment beeinflussen. Eine tierexperimentelle Studie aus dem Jahr 2014 weist darauf hin, dass ein übermäßiger Alkoholkonsum einen hemmenden Einfluss auf die Osteoklastenaktivität und damit die Zahnbewegung haben könnte [17], was jedoch in aktuellen Studien nicht bestätigt werden konnte [18,19]. Eine eigene Studie zeigte bezüglich des proinflammatorischen Expressionsprofils parodontaler Ligamentfibroblasten (PDLF) und der PDLF-gesteuerten Osteoklastogenese unter Einfluss mechanischer Kompression eher einen stimulierenden Einfluss von Alkohol [18], während eine tierexperimentelle Studie aus dem Jahr 2018 keine signifikanten Effekte von Alkohol auf die Zahnbewegung beobachten konnte [19].…”