Culture of human dental pulp cells at variable times post-tooth extraction

Abstract

The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.

El efecto del tiempo postexodoncia y la edad en la viabilidad celular total de la pulpa dental

“…En la actualidad existen escasas investigaciones que hayan abordado esta relación. [11][12][13][14] Parece ser que 24 horas es el tiempo postextracción adecuado para realizar cultivos. Sin embargo, no se ha establecido si se pueden realizar cultivos celulares exitosos de pulpa dental a las 72 horas postexodoncia.…”

“…10 El tiempo postexodoncia también es un factor muy importante; sin embargo, existen pocos estudios que contrastan este factor con la viabilidad celular. 14,15 Se ha reportado similar viabilidad en cultivos de CTPD a diferentes tiempos, 0, 0.5, 1, 2 y 5 horas postexodoncia. 14 También se han comparado cultivos realizados inmediatamente, y a las 24, 48, 72, 96 y 120 horas postexodoncia.…”

“…14,15 Se ha reportado similar viabilidad en cultivos de CTPD a diferentes tiempos, 0, 0.5, 1, 2 y 5 horas postexodoncia. 14 También se han comparado cultivos realizados inmediatamente, y a las 24, 48, 72, 96 y 120 horas postexodoncia. 15 Obteniendo como resultado que a menor tiempo, mayor fue la cantidad de células obtenidas.…”

Penetration of Silver Diamine Fluoride in Deep Carious Lesions of Human Permanent Teeth: An In Vitro Study

“…The protocol was approved by the Ethics Committee of the Faculty of Dentistry, Prince of Songkla University (EC6302-006). Extracted permanent teeth with deep cavities were rinsed thoroughly with tap water, kept in a plastic container, and then stored frozen in a refrigerator at –20°C within 5 hours of being extracted [ 17 ]. The carious lesion depth was assessed with a periapical radiograph.…”

Dental stem cell banking: Techniques and protocols

“…Other reported collection/transport solutions were HypoThermosol (BioLife Solutions), MesenCult basal medium (StemCell Technologies), and basic medium (alpha minimal essential medium [α‐MEM] supplemented with 10% fetal bovine serum [FBS]) (Karamzadeh et al, 2012; Perry et al, 2008). Regarding storage temperature, some articles refer to transportation or storage of teeth at 4°C (Daniela Ferreira Araújo et al, 2018; Gronthos et al, 2011; Karamzadeh et al, 2012).…”