Expression of E-cadherin and involucrin in leukoplakia and oral cancer: an immunocytochemical and immunohistochemical study

Abstract

To assess the immunocytochemical and immunohistochemical correlation of adhesion (E-cadherin) and cell differentiation (involucrin) molecules in oral leukoplakia and oral squamous cell carcinoma. Cytological samples and biopsies were obtained from male and female patients aged over 30 years with oral leukoplakia (n = 30) and oral squamous cell carcinoma (n = 22). Cell scrapings and the biopsy were performed at the site of the lesion and histological slides were prepared for the immunocytochemical analysis of exfoliated oral mucosal cells and for the immunohistochemical analysis of biopsy tissues using E-cadherin and involucrin. Spearman's correlation and kappa coefficients were used to assess the correlation and level of agreement between the techniques. Immunostaining for E-cadherin and involucrin by both techniques was similar in the superficial layers of the histological sections compared with cell scrapings. However, there was no statistical correlation and agreement regarding the immunocytochemical and immunohistochemical expression of E-cadherin and involucrin in oral leukoplakia (R = 0.01, p = 0.958) (Kappa = 0.017, p = 0.92) or in oral squamous cell carcinoma (R = 0.26, p = 0.206) (Kappa = 0.36, p = 0.07). The immunoexpression of E-cadherin and involucrin in tissues is consistent with the expression patterns observed in exfoliated oral mucosal cells, despite the lack of a statistically significant correlation. There is an association of the histopathological characteristics of leukoplakia with the expression E-cadherin and of the microscopic aspects of oral squamous cell carcinoma with immunohistochemical expression of involucrin.

Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders

“…However, expression of E-Cadherin in corneal cells in Normal oral Mucosa suggests for the functional role of E- cadherin in maintaining epithelial tissue integrity5 and loss of its expression indicates a normal desquamation. These observations in our study are consistent with the finding as that of Yogesh et al (2011),[ 15 ] Yuwanti et al (2011),[ 16 ] Ahmad et al (2013),[ 7 ] Zeidler et al (2014),[ 5 ] Sridevi et al (2015)[ 17 ] Abdalla et al (2017)[ 18 ] Da silva et al (2017)[ 8 ] and Fernandez et al (2017). [ 13 ]…”

“…However, such expression pattern was highlighted with respect to basal and parabasilar layer. These findings of the study was in accordance with the observations in studies by Yogesh et al (2011),[ 15 ] Yuwanti et al (2011),[ 16 ] Zeidler et al (2014), Abdalla et al (2017)[ 18 ] Da silva et al (2017)[ 8 ] and Fernandez et al (2017). [ 13 ] This decrease in E-Cadherin expression with increase in the severity of dysplasia may be a result of progression of dysplasia and a late event changing towards a cell phenotype with ability to invade (Yogesh et al ).…”

“…[ 7 ] Hence, identification of these markers may be a useful tool for prediction of malignant transformation. [ 8 ] Two such markers studied over the years with conflicting results are E-cadherin and vascular endothelial growth factor (VEGF).…”

Co-expression network-based identification of biomarkers correlated with the lymph node metastasis of patients with head and neck squamous cell carcinoma

“…More specifically, the immunohistochemical staining of IVL in cervical carcinoma in situ was remarkably lower than that in invasive cervical tumors [16]. For dysplastic leukoplakia, mild to moderate dysplasia presented relatively higher IVL expression, compared with leukoplakia with severe dysplasia, implying the participation of IVL in oral tissue malignant transformation [17]. PI3K signaling is a widely acknowledged important regulatory pathway in cancer-related processes, such as cell growth, survival and proliferation [18].…”

<p><em>PTHLH</em> Predicts the Prognosis of Patients with Oral Leukoplakia</p>

“…are considered highrisk factors for oral leukoplakia. [4][5][6][7] As a result, nonspecific treatments are used to cure the disease.…”