Patients with dental calculus have increased saliva and gingival crevicular fluid fetuin-A levels but no association with fetuin-A polymorphisms

Doğan, Gülnihal Emrem; Demir, Turgut; Laloğlu, Esra; Sağlam, Ebru; Aksoy, Hülya; Yıldırım, Abdülkadir; Akçay, Fatih

doi:10.1590/1807-3107bor-2016.vol30.0129

Cited by 6 publications

(7 citation statements)

References 32 publications

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“…These results were compared with those obtained using the commercial ELISA kit, 44.8 ± 0.8 ng/mL and 42.9 ± 0.6 ng/mL (n = 4), respectively. As it is obvious, an excellent agreement between the results obtained by both methods was apparent, what is also in agreement with that reported in the literature for saliva of healthy individuals, 41 ng/mL (Doğan al, 2016). All these results demonstrated the usefulness of the developed immunosensor for the analysis of HFA at low concentration levels in saliva with minimal sample treatment.…”

Section: Determination Of Hfa In Salivasupporting

confidence: 90%

“…The usefulness of the immunosensor in the analysis of real samples was tested by analyzing saliva from two smoker and non-smoker-volunteers. This type of sample was chosen because, as indicated in the Introduction section, HFA plays an important role in the detection of dental inflammatory processes (Doğan al, 2016). In addition, saliva is increasingly recognized as an attractive fluid for diagnostics since it allows a non-invasive cost-efficient sample collection method among other advantages (Campuzano et al, 2017).…”

Section: Determination Of Hfa In Salivamentioning

confidence: 99%

“…In addition, the synthesis of HFA is down-regulated during injury and inflammation (Choi, 2016) and plays an important role in blood vessel calcification (Schinke et al, 1996). As HFA acts as a potent inhibitor of calcium-phosphate precipitation and the calcification process, its levels are also related with dental calculus which is the first response in the inflammation of tooth supportive tissues (Doğan et al, 2016). Therefore, the relationship between HFA and the periodontal disease makes the determination of HFA in saliva relevant.…”

Section: Introductionmentioning

confidence: 99%

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Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva

Sánchez‐Tirado

González‐Cortés

Yáñez‐Sedeño

et al. 2018

Biosensors and Bioelectronics

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This paper reports the development and performance of an electrochemical immunosensor using magnetic multiwalled carbon nanotubes (m-MWCNTs) as nanocarrier tags for the determination of human fetuin A (HFA), a relevant biomarker of obesity, insulin resistance, and type-2 diabetes as well as for pancreatic and liver cancers and inflammatory processes. Screen-printed carbon electrodes were grafted with p-aminobezoic acid and streptavidin was covalently immobilized on the electrode surface. A biotinylated capture antibody was immobilized through streptavidin-biotin interaction and a sandwich assay configuration was implemented using m-MWCNTs conjugated with HRP and anti-HFA antibodies as the detection label. The determination of HFA was accomplished by measuring the current produced by the electrochemical reduction of benzoquinone at -200 mV upon addition of HO as HRP substrate. The prepared m-MWCNTs were characterized by SEM, TEM, XRD and EDS. All the steps involved in the immunosensor preparation were monitored by electrochemical impedance spectroscopy and cyclic voltammetry. A linear calibration plot for HFA was found between 20 and 2000 pg/mL with a LOD value of 16 pg/mL. This performance is notably better than that reported for an ELISA kit and a chronoimpedimetric immunosensor. The favorable contribution of m-MWCNTs in comparison with MWCNTs without incorporated magnetic particles to this excellent analytical performance is also highlighted. The immunosensor selectivity against other proteins and potentially interfering compounds was excellent. In addition, the usefulness of the immunosensor was demonstrated by the analysis of HFA in saliva with minimal sample treatment.

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Section: Determination Of Hfa In Salivasupporting

confidence: 90%

Section: Determination Of Hfa In Salivamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva

Sánchez‐Tirado

González‐Cortés

Yáñez‐Sedeño

et al. 2018

Biosensors and Bioelectronics

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“…Fetuin‐A is an inhibitor of calcification and inhibits the collapse of calcium phosphate crystals and plays a role in regulating bone mineralization by preventing ectopic calcification (Türer, Ballı, & Güven, ). In a study investigating whether fetuin‐A values are effective in the presence of dental calculus, salivary fetuin‐A levels were detected in patients with dental calculus and it was defined that this situation might result from an adaptive mechanism to inhibit mineral precipitation and eventually calculus formation (Doğan et al, ). Fetuin‐A protein, in addition to being an inhibitor of calcification, acts as a TGF‐ antagonist (Demetriou, Binkert, Sukhu, Tenenbaum, & Dennis, ).…”

Section: Introductionmentioning

confidence: 99%

Salivary fetuin‐A, S100A12, and high‐sensitivity C‐reactive protein levels in periodontal diseases

et al. 2018

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“…excluded articles). Twelve studies enrolling 491 participants were finally selected(Camargo et al, 2013;Cirano et al, 2012;da Cruz et al, 2008;Dag, Firat, Arikan, Kadiroglu, & Kaplan, 2009;Dogan et al, 2016;Kara, Cifcibasi, Karsidag,…”

mentioning

confidence: 99%

Impact of diabetes on clinical periodontal outcomes following non‐surgical periodontal therapy

Hsu

Nair

Angelov

et al. 2019

J Clinic Periodontology

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Aim This systematic review aimed to evaluate the impact of diabetes mellitus on clinical outcomes of non‐surgical periodontal therapy. Materials and Methods Searches were conducted in electronic databases to screen studies published from January 1960 to August 2018. The included studies had at least two groups of patients: chronic periodontitis only (P) or both diabetes and chronic periodontitis (DMP). Outcomes of interest included the difference between the two groups in probing depth (PD) reduction and clinical attachment level (CAL) gain following non‐surgical periodontal therapy. Meta‐regression was conducted to evaluate the correlation between the outcomes of interest and contributing factors. Results A total of 12 studies with a follow‐up period up to 6 months were included. There was no significant difference in PD reduction (p = 0.55) or CAL gain (p = 0.65) between the two groups. A positive association between PD reduction and baseline PD difference (p = 0.03), and a negative association between PD reduction and age (p = 0.04) were found. The level of HbA1c at baseline did not significantly affect the difference in PD reduction (p = 0.39) or CAL gain (p = 0.44) between two groups. Conclusions Recognizing the study's limitations, we conclude that diabetes mellitus (HbA1c ≤ 8.5%) does not appear to significantly affect short‐term clinical periodontal outcomes of non‐surgical periodontal treatment.

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Patients with dental calculus have increased saliva and gingival crevicular fluid fetuin-A levels but no association with fetuin-A polymorphisms

Abstract: Biochemistry, Dentistry, Periodontics(a) Private practice, Erzurum, Turkey.

Cited by 6 publications

References 32 publications

Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva

Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva

Salivary fetuin‐A, S100A12, and high‐sensitivity C‐reactive protein levels in periodontal diseases

Impact of diabetes on clinical periodontal outcomes following non‐surgical periodontal therapy

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