Bacterial diversity of symptomatic primary endodontic infection by clonal analysis

Abstract

The aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. Samples were collected from 10 patients and submitted to bacterial DNA isolation, 16S rRNA gene amplification, cloning, and sequencing. A bacterial genomic library was constructed and bacterial diversity was estimated. The mean number of taxa per canal was 15, ranging from 11 to 21. A total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) were different species and 29 (38.15%) were taxa reported as yet-uncultivable or as yet-uncharacterized species. Prevotella spp., Fusobacterium nucleatum, Filifactor alocis, and Peptostreptococcus stomatis were the most frequently detected species, followed by Dialister invisus, Phocaeicola abscessus, the uncharacterized Lachnospiraceae oral clone, Porphyromonas spp., and Parvimonas micra. Eight phyla were detected and the most frequently identified taxa belonged to the phylum Firmicutes (43.5%), followed by Bacteroidetes (22.5%) and Proteobacteria (13.2%). No species was detected in all studied samples and some species were identified in only one case. It was concluded that acute primary endodontic infection is characterized by wide bacterial diversity and a high intersubject variability was observed. Anaerobic Gram-negative bacteria belonging to the phylum Firmicutes, followed by Bacteroidetes, were the most frequently detected microorganisms.

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

“…4 On the basis of the results of previous studies, the microbial communities in primary endodontic infections are more diverse than those in secondary endodontic infections. 5,6,7 Moreover, variation in the microorganisms isolated in several studies is likely a result of differences in the sampling techniques, laboratory procedures, and diagnostic approaches. 8 Maryam POURHAJIBAGHER (a) Roghayeh GHORBANZADEH (b) Abbas BAHADOR (a) (a) Tehran University of Medical Sciences, Department of Microbiology, School of Medicine, Tehran, Iran.…”

Effects of plasma jet, dielectric barrier discharge, photodynamic therapy and sodium hypochlorite on infected curved root canals

“…Additionally, specimens were incubated with E. faecalis for 6 d, resulting in a thin biofilm . When thinking about in vivo situations of apical periodontitis, biofilms are older, thicker and even more diverse regarding the species of microorganisms present in the root canal . CAP I and PDT were significantly more effective than CAP II, which can be explained by its form of application, as it cannot be inserted directly into the root canal in its present form.…”

Correlation Between Volume of Root Canal, Cultivable Bacteria, Bacterial Complexes and Endotoxins in Primary Infection

“…Knowledge of the pathogenesis of endodontic infections is important for establishing therapeutic strategies (1). Special attempt has been given for the knowledge bacterial species and their association in complexes (2)(3)(4)(5).…”

“…Studies have demonstrated a wide diversity of bacteria species, thus elucidating the polymicrobial profile involving both Gram-positive and Gram-negative bacteria species in endodontic infections (2,4). Interactions of bacteria species and their grouping with complexes make endodontic infections even far more complex to the immune system response (6), which can lead to different clinical symptoms (5).…”