Clinical effect of photodynamic therapy on primary carious dentin after partial caries removal

Abstract

This study was conducted to assess the clinical effect of photodynamic therapy (PDT) in the decontamination of the deep dentin of deciduous molars submitted to partial removal of carious tissue. After cavity preparation, dentin samples were taken from the pulp wall of nineteen deciduous molars before and after PDT application. Remaining dentin was treated with 0.01% methylene blue dye followed by irradiation with an InGaAlP diode laser (λ -660 nm; 40 mW; 120 J/cm 2 ; 120 s). Dentin samples were microbiologically assessed for the enumeration of total microorganisms, Lactobacillus spp. and mutans streptococci. There was no significant difference in the number of colony-forming units (CFU) for any of the microorganisms assessed (p > 0.05). Photodynamic therapy, using 0.01% methylene blue dye at a dosimetry of 120 J/cm 2 would not be a viable clinical alternative to reduce bacterial contamination in deep dentin.

Antimicrobial photodynamic therapy as an adjunct for treatment of deep carious lesions—A systematic review

“…Finally, seven eligible studies in English language published from 1 st January, 2011 until the 21 st June, 2016 were selected for the final review [14,[22][23][24][25][26]. One study consequently had to be excluded due to the inadequate description of microbiological sampling procedures [22].…”

“…In total, six in vivo studies were included in this review. In five of these reports aPDT was used to aid the treatment of occlusal dentine caries lesions (class I) in primary and permanent molars of children [14,23,[25][26][27], while two studies involved supportive use of aPDT in treatment of dentine caries lesions (class I, class II) in permanent teeth of adults [24,27]. The number of aPDT-treated teeth in all studies ranged between 10 -32 among child and 12 -90 among adult patients.…”

“…The number of aPDT-treated teeth in all studies ranged between 10 -32 among child and 12 -90 among adult patients. The cavity preparation initially involved the removal of dentine from the lateral walls of carious lesions, which was performed with high-speed hand-pieces and burs [24,25] or excavators [14,23,27]. Thereafter, the removal of carious dentine from the pulp walls was conducted with low-speed carbide burs [23][24][25] or excavators [26,27].…”

The Antibacterial Potentiality of Photo-activated Disinfection Versus a Chemical Disinfection Solution on Carious Dentin

“…There was no significant difference in the number of colonyforming units (CFU) for any of the microorganisms assessed. Photodynamic therapy using 0.01% methylene blue dye at a dosimetry of 120 J/cm 2 would not be a viable clinical alternative to reduce bacterial contamination in deep dentin (10) .…”

Eficácia do uso de lasers ou clorexidina na redução microbiana em lesões de cárie produzidas artificialmente

“…Além dos lasers de alta potência, existem ainda no mercado os lasers de baixa potência, mais acessíveis comercialmente e resultados positivos para redução bacteriana, quando associados a um fotossensibilizador. A técnica denominada Terapia Fotodinâmica Antimicrobiana (aPDT) (Da Mota et al, 2016) baseia-se na aplicação de uma fonte de luz sobre um tecido alvo fotossensibilizado (uso de fotossensibilizadores) que, na presença de oxigênio, produz oxigênio singleto e radicais livres que danificam irreversivelmente os componentes celulares e modificam suas atividades metabólicas, levando à morte celular (Neves et al, 2016;Gugliemi et al, 2011).…”