Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals

Abstract

Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals Abstract: The objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with Enterococcus faecalis. Forty-five human lower premolars were infected with a culture of E. faecalis (ATCC 29212) for 28 days. Five other teeth that were neither contaminated nor instrumented were used as controls. The 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (K-type, Dentsply Maillefer, Ballaigues, Switzerland); rotary (MTwo, VDW GmbH, Munich, Germany); and reciprocating (Reciproc R50, VDW GmbH, Munich, Germany) instruments. During chemomechanical preparation, 21 mL of 2.5% NaOCl was used as the irrigating solution. Microbiological sampling was performed before (S1) and immediately after (S2) the chemomechanical preparation using sterilized paper points. Specimens were then cleaved, and 0.02 g of dentine chips was collected from the root thirds to verify the presence of microorganisms in dentinal tubules. All three preparation techniques reduced the number of microorganisms in the root canal lumen and dentine chips from the root thirds, but no significant differences were observed between the three groups (p > 0.05). Reciprocating instrumentation with Reciproc R50 was effective in reducing the number of microorganisms within the root canal system. Although this technique involves the use of only one file to perform the root canal therapy, it is as effective as conventional rotary instrumentation in reducing the E. faecalis biofilm from the root canal system. However, further clinical investigations are warranted in order to ratify these results.

Evaluation of the Effect of Rotary Systems on Stresses in a New Testing Model Using a 3-Dimensional Printed Simulated Resin Root with an Oval-shaped Canal: A Finite Element Analysis Study

“…Among the teeth data collected by computed tomographic imaging, mandibular second premolar teeth with single oval-shaped canals; straight roots; and no internal resorption, root dilacerations, calcifications, or previous endodontic treatment were selected. The ratios between the buccolingual and mesiodistal dimensions of the root canals were greater than 2:1 when measured 5 mm from the root apex (20).…”

The efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique

“…Various methodologies can be used to collect microorganisms in the root canal, such as the use of paper point technique [16] and the collection of dentinal samples from different thirds of the root canal walls [28]. In the present research, the microbial samples collected within the root canals with sterile paper points were obtained before and after final disinfection procedures.…”

“…A concern has been presented, however, regarding the ability to disinfect the root canal promoted by this technique, when shorter contact However, to date, sterilization of infected root canal systems cannot be achieved in vivo by any known instrumentation technique or irrigation protocol. Also, the minimum bacterial load remaining inside the root canal, necessary to promote the healing of periapical tissues or for the maintenance of their health, is still unknown [28].…”

Nickel–titanium instruments in endodontics: a concise review of the state of the art

“…Currently, the Reciproc system is one of the most popular instruments in endodontics. Previous research suggests it provides excellent in vitro and in vivo results in several aspects, including mechanical properties, 104,116,107,108 disinfection of root canals, 109 root canal shaping, 32,110 and postoperative pain. 105,111,112 The Reciproc blue CM (VDW GmbH, Munich, Germany) thermally treated NiTi instruments are improved versions of the original Reciproc system.…”