Homeobox B2 is a potential prognostic biomarker of glioblastoma

Abstract

SUMMARY OBJECTIVES HOXB2 is a new prognostic indicator for lung cancer. But it is unclear whether HOXB2 holds an effect in glioblastoma (GBM) progression. The purpose of this article was to probe the influences of HOXB2 on GBM pathogenesis. METHODS HOXB2 expression level and prognostic power in GBM patients were analyzed. Then the mRNA and protein expression levels of HOXB2 in GBM cell lines were tested by qRT-PCR and western blotting. Cell proliferation, invasion, and migration were determined by CCK8 and transwell assay, severally. The protein levels of PI3K/AKT-pathway associated proteins were analyzed by western blotting. RESULTS The results indicated that HOXB2 was distinctly overexpressed in GBM patients and high expression of HOXB2 was related to a poor prognosis. Moreover, the expression of HOXB2 was higher in all GBM cell lines U251, U-87MG, GOS-3 than that in HEB cells (normal control). Meanwhile, decreased expression of p-PI3K and p-AKT were identified after HOXB2 knockdown. CONCLUSIONS These data demonstrated that HOXB2 had a vital role in GBM progression and could serve as a promising target for GBM treatment.

Transcription factor HOXB2 upregulates NUSAP1 to promote the proliferation, invasion and migration of nephroblastoma cells via the PI3K/Akt signaling pathway

“…Therefore, it was hypothesized that HoXB2 overexpression could abrogate the inhibitory effect of nuSaP1 knockdown on the proliferation and metastasis of nephroblastoma cells via the Pi3K/akt signaling pathway. consistent with the aforementioned findings, li et al (29) showed that the expression levels of p-Pi3K and p-akt were decreased in glioblastoma cells following HoXB2 knockout. These results suggested that HoXB2 could promote the activation of Pi3K/akt signaling.…”

Molecular implications of HOX genes targeting multiple signaling pathways in cancer

“…The knockdown of HOXB2 in GBM cell lines resulted in a reduction of proliferation and invasion via the PI3K/ AKT pathway. The involvement of the PI3K pathway was confirmed by the increased levels of p-PI3K and p-AKT upon HOXB2 overexpression (Li et al 2020c).…”

Há Relação No Comprimento Dos Dedos Com O Desempenho No Powerlifting Paralímpico?

“…O Gene Homeobox codifica os fatores de transcrição com funções reguladoras no desenvolvimento animal e vegetal (26,28). São também codificadas proteínas nucleares que funcionam como fatores de transcrição e controlam aspectos da morfogênese e diferenciação celular durante o desenvolvimento embrionário normal em vários animais (33) .…”