Thrombopoietin is associated with a prognosis of gastric adenocarcinoma

Abstract

SUMMARY OBJECTIVE Thrombopoietin (THPO) is well-known as a megakaryocyte growth and development factor (MGDF) involved in megakaryocyte proliferation and maturation. To explore the biological effects of THPO in gastric adenocarcinoma, we conducted this study. Methods: By accessing the TCGA database, the expression level of THPO was determined in tumor tissues. The association between THPO expression and clinical features, or prognostic significance was described by Cox regression analysis and Kaplan-Meier. The SiRNA method was used to decline the THPO expression; then cell viability, invasion, and migration were detected to verify the effects of the knockdown of THPO. qPCR and western blotting were implemented to examine the expression level of THPO. Results: The expression of THPO was increased in tumor tissue and cells, its high-regulation was associated with a poor prognosis in patients with gastric adenocarcinoma. Cell viability, invasion, and migration were suppressed in AGS with the down-regulation of THPO. Furthermore, on the basis of si-THPO transfection, E-cadherin was promoted while N-cadherin and Vimentin were attenuated. CONCLUSION Our results revealed that THPO may be a potent marker of gastric adenocarcinoma, providing a novel potential screening method for gastric adenocarcinoma.

ELF3‐AS1 contributes to gastric cancer progression by binding to hnRNPK and induces thrombocytosis in peripheral blood

“…J, The levels of ELF3-AS1, hnRNPK, and CCL20 in gastric cancer tissues were positively correlated were potential downstream target genes for ELF3-AS1. Although several studies have reported that tumor tissues and cells can produce THPO,51,52 and we failed to verify the positive correlation between THPO and platelet count in the peripheral blood of GC patients, our research indicates the possibility that the high expression ELF3-AS1 in GC tissues promotes the expression and release of IL-6…”

“… 33 In our study, we found that high ELF3‐AS1 expression was associated with thrombocytosis, and IL6 and THPO were potential downstream target genes for ELF3‐AS1. Although several studies have reported that tumor tissues and cells can produce THPO, 51 , 52 and we failed to verify the positive correlation between THPO and platelet count in the peripheral blood of GC patients, our research indicates the possibility that the high expression ELF3‐AS1 in GC tissues promotes the expression and release of IL‐6 into the blood and stimulates the liver to produce more THPO, or that GC tissues directly produce more THPO, resulting in an increase in blood platelet count. Verification of this hypothesis requires more research.…”

Construction of a prognostic model for gastric cancer patients based on NAD+ metabolism-related genes and tumor microenvironment analysis

“…Thrombopoietin (THPO) is involved in megakaryocyte proliferation and maturation of megakaryocyte growth and development factors. It is overexpressed in GC and causes poor prognosis by affecting cell viability, invasion, and migration [19]. In recurrent GC, elevated UPK1B expression has a worse prognosis [20].…”

Does diabetes affect paraneoplastic thrombocytosis in colorectal cancer?

“…Research on CRC and thrombopoietin is limited. Earlier studies have identified that cancer cells can induce circulating thrombopoietin production [ 50 ]; furthermore, higher thrombopoietin level is associated with gastrointestinal cancers, and a possible relationship between more advanced clinical stages and thrombopoietin has been also suggested [ 51 , 52 ]. Similar to the observations above, we found that the thrombopoietin level of CRC patients is higher than those of healthy subjects; however, the effect of thrombopoietin levels over survival was negligible, and no change with the course of the disease could have been identified in either of our longitudinal models.…”