ELF3‐AS1 contributes to gastric cancer progression by binding to hnRNPK and induces thrombocytosis in peripheral blood

Song, Shubin; He, Xu; Wang, Jing; Wang, Rong; Wang, Leilei; Zhao, Wei; Wang, Yimin; Zhang, Yongle; Yu, Zhiyong; Miao, Dengshun; Xue, Yingwei

doi:10.1111/cas.15104

Cited by 5 publications

(3 citation statements)

References 49 publications

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“…To verify the binding of hnRNPU and IL21-AS1, we performed the RNA immunoprecipitation (RIP) by hnRNPU antibody and five lncRNAs including UCA1, MIR100HG, lincRNA-EPS, lincRNA-AK023096 and ELF3-AS1 were used as negative controls. [15][16][17][18][19] IL21-AS1 was identified in RNA enriched by hnRNPU antibody, but no significant enrichment of the five lncRNAs, which confirmed the interaction between IL21-AS1 and hnRNPU protein in Tfh cells (Figures 5E and S6). We predicted a binding fragment (2543-2708 nt) with hnRNPU protein in IL21-AS1 sequence by CatRAPID software (Figure S5C).…”

Section: Il21-as1 Binds To Il21 Promoter and Hnrnpu Protein In Tfh Cellssupporting

confidence: 57%

“…Previous study has shown that hnRNPU could enhance the function of Blnc1/EBF2 ribonucleoprotein complex and physically interact with lncRNA Blnc1 in human and mouse. To verify the binding of hnRNPU and IL21‐AS1, we performed the RNA immunoprecipitation (RIP) by hnRNPU antibody and five lncRNAs including UCA1, MIR100HG, lincRNA‐EPS, lincRNA‐AK023096 and ELF3‐AS1 were used as negative controls 15–19 . IL21‐AS1 was identified in RNA enriched by hnRNPU antibody, but no significant enrichment of the five lncRNAs, which confirmed the interaction between IL21‐AS1 and hnRNPU protein in Tfh cells (Figures 5E and S6).…”

Section: Resultsmentioning

confidence: 66%

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LncRNA IL21‐AS1 interacts with hnRNPU protein to promote IL21 overexpression and aberrant differentiation of Tfh cells in systemic lupus erythematosus

Liu

Long

et al. 2022

Clinical & Translational Med

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Background The aberrant differentiation of T follicular helper (Tfh) cells plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). However, the mechanism of regulating Tfh cells differentiation remains unclear. Long noncoding RNAs (lncRNAs) act as important regulators in the processes of innate and adaptive immune response. Whether lncRNAs are involved in regulating Tfh cell differentiation and autoimmune responses need to be further identified. Methods The characters and functions of human IL21‐AS1 and its mouse homologous lncRNA (mIl21‐AS) were investigated by a series of biochemical assays and cell transfection assay. mIl21‐AS1 regulating humoral immune response in vivo was explored by keyhole limpet haemocyanin (KLH) and chronic graft versus host disease (cGVHD) model. Results Human IL21‐AS1 and its mouse homologous lncRNA (mIl21‐AS) were identified and cloned. We uncovered that IL21‐AS1 was highly expressed in CD4+ T cells of SLE patients and Tfh cells, which promoted differentiation of Tfh cells. Mechanistically, IL21‐AS1 bound heterogeneous nuclear ribonucleoprotein U and recruited acetyltransferases CREB‐binding protein to the promoter of IL21, leading to the transcriptional activation of IL21 and Tfh cells differentiation through increasing Histone H3 acetylation level on IL21 promoter. Moreover, Tfh proportion and antibodies production were significantly increased in mIl21‐AS knock‐in mice immunized with KLH. mIl21‐AS1 overexpression also exacerbated the lupus‐like phenotype in cGVHD mice model. Conclusions Our results demonstrate that IL21‐AS1 activates IL21 transcription via epigenetic mechanism to promote germinal centre response, adding insight into the molecular regulation of autoimmune pathogenesis and providing a novel target for SLE treatment.

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Section: Il21-as1 Binds To Il21 Promoter and Hnrnpu Protein In Tfh Cellssupporting

confidence: 57%

Section: Resultsmentioning

confidence: 66%

LncRNA IL21‐AS1 interacts with hnRNPU protein to promote IL21 overexpression and aberrant differentiation of Tfh cells in systemic lupus erythematosus

Liu

Long

et al. 2022

Clinical & Translational Med

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“…Lnc-ELF3-AS1 is highly expressed in gastric cancer tissues and indicates a poor prognosis. ELF3-AS1 may regulate the downstream target gene CC motif chemokine 20 by binding to the RNA binding protein hnRNPK to promote cell proliferation, migration and epithelial stroma Transform and inhibit cell apoptosis ( 87 ). Lnc-HoxBlinc can be combined with the promoter region of the characteristic gene of NPM1c, through MLL1 recruitment and promoter H3K4me3 modification to control their activation in HoxBlincTg HSPC, and then act as a cancer-promoting factor to cause the malignant progression of acute myeloid leukemia (AML) ( 88 ).…”

Section: Overview Of Lncrnasmentioning

confidence: 99%

The Emerging Landscape of Long Non-Coding RNAs in Wilms Tumor

Liu

2022

Front. Oncol.

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Long noncoding RNAs (LncRNAs) are transcripts of nucleic acid sequences with a length of more than 200 bp, which have only partial coding capabilities. Recent studies have shown that lncRNAs located in the nucleus or cytoplasm can be used as gene expression regulatory elements due to their important regulatory effects in a variety of biological processes. Wilms tumor (WT) is a common abdominal tumor in children whose pathogenesis remains unclear. In recent years, many specifically expressed lncRNAs have been found in WT, which affect the occurrence and development of WT. At the same time, lncRNAs may have the capacity to become novel biomarkers for the diagnosis and prognosis of WT. This article reviews related research progress on the relationship between lncRNAs and WT, to provide a new direction for clinical diagnosis and treatment of WT.

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