Polymorphisms of the GSTT1 and GSTM1 genes in polycystic ovary syndrome

Abstract

SUMMARY BACKGROUND: This study aimed to investigate the deletion polymorphisms of the genes of the glutathione S-transferase family GSTT1 and GSTM1 in patients with Polycystic Ovarian Syndrome (PCOS), comparing them with a control population. METHODS: Blood was collected from 219 women (110 with PCOS and 109 controls) and genomic DNA was extracted. For the analysis of polymorphisms, the technique used was multiplex PCR. In the statistical analysis, the chi-square test and multiple logistic regression were used. RESULTS: There is no association between the GSTM1 null and GSTT1 null genotypes with PCOS when analyzed separately (P = 0.616 and P = 0.188). The analysis of the combined genotypes showed differences between the groups (P < 0.05), evidencing that the genotypic combination GSTT1 positive and GSTM1 negative is more frequent among patients. In the multivariate analysis, smoking was more frequent in the control group (OR = 0.22; 95% CI - 0.87-0.57; P = 0.002) while the presence of a family history of PCOS (OR = 2, 96; 95% CI - 1.54-5.68; P = 0.001) was more frequent in women with PCOS. CONCLUSIONS: In the studied sample, the deletion polymorphisms of the GSTT1 and GSTM1 genes isolated are not associated with PCOS, but in combination, they may be implicated in the etiology of the condition.

Individual effects of GSTM1 and GSTT1 polymorphisms on the risk of polycystic ovarian syndrome: A systematic review and meta-analysis

“…The findings of our investigation agree with those of most prior studies. Babu et al ( 17 ) , Savić-Radojević et al ( 18 ) , Chung et al ( 19 ) , and Azevedo et al ( 20 ) are similar to the results of our study. We could not find an association between the polymorphisms of GSTM1 and GSTT1 and increased risk of PCOS.…”

“…The investigation consisted of five case-control studies, with 872 cases and 735 controls. The primary focus of this study was to explore the relationship between GSTM1 and GSTT1 null deletion polymorphisms and the risk of PCOS ( 17 , 18 , 19 , 20 , 21 ) . Table 1 shows that two studies were conducted in Asia, two in Europe, and one in South America.…”

Síndrome de ovários policísticos (SOP), fisiopatologia e tratamento, uma revisão

“…O estresse oxidativo também é um fator constituinte da fisiopatologia da SOP. O estresse oxidativo estimula a expressão de genes envolvidos na esteroidogênese e proliferação das células da teca (Azevedo et al, 2021). Assim, polimorfismos em genes relacionados à desintoxicação de espécies reativas de oxigênio podem alterar as funções das enzimas que atuam sobre o metabolismo dessas moléculas, nesse caso, enzimas da família glutationa S-transferase.…”

“…Assim, polimorfismos em genes relacionados à desintoxicação de espécies reativas de oxigênio podem alterar as funções das enzimas que atuam sobre o metabolismo dessas moléculas, nesse caso, enzimas da família glutationa S-transferase. Tais polimorfismos reduzem ou eliminam as atividades dessas enzimas e podem aumentar as suscetibilidades a doenças, incluindo SOP (Azevedo et al, 2021).…”

Detection of GSTM1-null Genotype in Women Undergoing IVF Treatment

“…Like the other members of the glutathione-S-transferases family, GSTM1 act also as a hormone binding protein. The corresponding encoding sequence is well recognized for its high polymorphism [13][14][15]. Due to an extended deletion of the gene (approximately 10 kb), a null activity allele is produced, leading to a non-functional protein [4,12,16].…”