Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration

Abstract

SUMMARY Mirabegron is a kind of β3 adrenergic receptor agonist which is an effective drug for the treatment of overactive bladder. In this research, a UPLC–MS/MS method is developed and validated for the study of mirabegron pharmacokinetic in rats. A protein precipitation method is applied for sample preparation with acetonitrile. m/z 397.3→379.6, m/z 326.4→121.0 for mirabegron, tolterodine (IS), respectively in the positive ion mode was performed for quantitation. The method is reliable and reproducible in our study (intra-day precision≤11.06%, inter-day precision≤11.43%) with concentration curves linear from 5 to 2500 ng/mL(R2>0.999). Stability studies demonstrated that mirabegron was stable under a variety of storage conditions. This method was successfully applied for determining mirabegron in rats after oral and intravenous administration.

Electrochemical Determination of Mirabegron at Silica Modified Carbon Paste in Micellar Medium: Sensitive Application with Drug Product and Spiked Plasma

“…Literature review showed different methods for the determination of MIR using UV spectrophotometric [3] , spectrofluorometric [4] , chromatographic methods [5–7] LC‐MS/MS [8,9] .…”