LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

Souza, Denys Matheus Santana Costa; Xavier, Aloísio; Otoni, Wagner Campos; Miranda, Natane Amaral; Maggioni, Joane Helena

doi:10.1590/1806-90882018000600004

Cited by 16 publications

(11 citation statements)

References 19 publications

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“…4a) (p < 0.05). These results are close to the ones reported by Souza et al (2018) HYP has been associated with the micropropagation of woody species, which can influence crop development. This outcome may be linked to cultivation factors such as the use of a liquid medium in bioreactor systems, which tend to increase water and mineral salts' absorption by plants.…”

Section: Resultssupporting

confidence: 91%

Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

et al. 2020

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Spectral quality is an important factor for in vitro development of explants in a bioreactor system. Based on the need to optimize micropropagation for E. grandis × E. urophylla clones, the aim of the study was to assess the spectral quality of in vitro multiplication in temporary immersion bioreactor (TIB). The tissue used to generate the explants (i.e., the nodal segment with 1 cm of length and two axillary bud without leaves) was previously in vitro established and multiplied, it derived from ministumps of E. grandis × E. urophylla clone grown in a semi-hydroponic system. The spectral quality of in vitro multiplication was assessed through five light sources (i.e., fluorescent lamp, red, green, blue, and yellow cellophane). Morphological and anatomical features of tissues grown in TIB were evaluated at 90 days. Based on the results, yellow and blue spectral qualities were the most suitable to be adopted for in vitro multiplication of E. grandis × E. urophylla, since they enabled lesser hyperhydricity, favors high number of shoots per explant and shoot length, as well as thicker mesophyll and spongy parenchyma; arise as an alternative for large-scale production of eucalypts clonal plants.

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Section: Resultssupporting

confidence: 91%

Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

et al. 2020

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“…In the present study on C. trichotoma, the oxidation percentage increased directly in relation to the use of sodium hypochlorite; the higher the concentration of the disinfecting agent, the higher the percentage of oxidation, thus indicating that the tissues of this species exhibited high sensitivity to chemical treatment. Similar results have been reported in studies on E. cloeziana (Oliveira et al 2015), E. benthamii (Baccarin et al 2015), Corymbia citriodora × C. torelliana and C. torelliana × C. citriodora (Souza et al 2018), and E. grandis × E. urophylla .…”

Section: Discussionsupporting

confidence: 90%

Emission of epicormic shoots and in vitro establishment of Cordia trichotoma selected adult trees

et al. 2022

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Cordia trichotoma, popularly known in Brazil as 'louro-pardo', has numerous environmental and timber-related applications. This species is naturally propagated by seeds; however, this form of propagation results in restricted seedling production due to high genetic variability associated with a low degree of forest improvement. Thus, the cloning of selected adult trees is an alternative for producing high-quality plants. The aims of this work were to evaluate the emission of epicormic shoots from pruned branches and to define a protocol for the in vitro establishment of C. trichotoma selected adult trees. Propagules used in these experiments were derived from the selection of four C. trichotoma adult trees. The branches were placed in a greenhouse to induce epicormic shoots. Number of shoots according to the climatic season (autumn and spring) and shoot collection time were evaluated. Three sodium hypochlorite concentrations were evaluated for five minutes (C 1 = 0.58 % active chlorine, C 2 = 1.12 % active chlorine, and C 3 = 2.12 % active chlorine) for in vitro establishment. Results indicated that the epicormic shoot technique could be considered viable for shoot emission of C. trichotoma, regardless of the climatic season (autumn or spring). Sodium hypochlorite solution applied for 5 minutes (1.12 % of active chlorine) was effective for the in vitro establishment of nodal segments from epicormic shoots, resulting in 61.3 % of established explants. These results are promising for the cloning of adult trees during the mature stage of development in the field without the need to cut the selected tree.

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“…Plants grown under white LEDs and red/blue LEDs induced higher number of shoots (0.85 and 0.67, respectively) than the other light qualities ( Figure 1A). Similar results were observed in Saccharum officinarum, which in white LEDs promoted a higher number of shoots (Ferreira et al, 2016) and under red/blue LEDs in Corymbia (Souza et al, 2019).…”

Section: Resultssupporting

confidence: 78%

Impact of MS concentration and light qualities on alpine red ginger elongation and rooting in vitro

Pinheir

Schmidt

Araujo

et al. 2020

PCCM

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Some factors in in vitro cultivation, such as formulation of the culture medium and the quality of the light source, may vary the response of the plants in those conditions, favoring the photosynthetic metabolism obtaining more rustified plants capable of benefiting from the conditions of ex vitro cultivation. Thus, the objective was to evaluate in vitro elongation/rooting of Alpinia purpurata cv. Red Ginger under different light-emitting diodes (LEDs) and saline concentration of the MS medium. For this, previously established in vitro plants were maintained for 55 days in 550 mL glass flasks containing Carolina® substrate and MS culture medium with different salt concentrations, under LEDs qualities. The following parameters were evaluated: number of green and senescent leaves, shoot length, length of the largest root, fresh and dry mass of leaves, stem and root, root volume, photosynthetic pigments [chlorophyll a, chlorophyll b, total chlorophylls (a+b) and carotenoids (µg cm-2)], stomatal density and acclimatization percentage (after 33 days of ex vitro cultivation). After 55 days, plants grown under red LEDs and full-strength MS culture medium is indicated and promote efficient shoot length and photosynthetic pigment content, respectively. These conditions provided plants better adapted for ex vitro acclimatization.

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LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

Cited by 16 publications

References 19 publications

Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

Emission of epicormic shoots and in vitro establishment of Cordia trichotoma selected adult trees

Impact of MS concentration and light qualities on alpine red ginger elongation and rooting in vitro

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