DNA Profiles of Salmonella Spp. Isolated from Chicken Products and From Broiler and Human Feces

Abstract

Salmonella species have been isolated from various kinds of food and are accountable for outbreaks of foodborne diseases in humans. This study aimed at identifying the similarities between the DNA profiles of Salmonella isolated from chicken feces, chicken products, and human feces in southern Brazil. Six hundred samples were collected (200 from chicken products, 200 from broiler chicken feces, and 200 from human feces) and tested for the presence of Salmonella. Isolates proven to be Salmonella compatible by biochemical and serological tests were tested by the Polymerase Chain Reaction. Their DNA profiles were then analyzed by PFGE and rep-PCR. Salmonella was isolated from 16 out of 600 analyzed samples, with Schwarzengrund serotype presenting the highest incidence, followed by Mbandaka in chicken meat and fecal samples, and Panama in human fecal samples. Some strains isolated from chicken fecal and product samples were indistinguishable by the molecular methods used in the study, suggesting that that the contamination of the broilers on the farm can be transmitted the processed products.

Molecular detection of virulence genes in Salmonella spp. isolated from chicken faeces in Mafikeng, South Africa

“…Six Salmonella serovars were isolated, namely S. Typhimurium, S. Heidelberg, S. bongori, S. Enteritidis, S. Paratyphi B and S. Newport. All these serovars are known to be pathogenic in humans and cause salmonellosis (Fagbamila et al 2017 ; Orji, Onuigbo & Mbata 2005 ; Tejada et al 2016 ). Among the Salmonella isolates detected from this study, S. Typhimurium, S. Enteritidis and S. Newport have previously been reported in raw broiler samples by another study here in Mafikeng, South Africa (Olobatoke & Mulugeta 2015 ).…”

Ocorrência de Campylobacter e Enterobacteriaceae em aves silvestres e frangos de corte

“…Oito Campylobacter isolados de frangos foram identificados como C. jejuni, e dois como C. coli. A presença de Campylobacter já tem sido reportada em frangos da região (Gomes et al, 2006;Silva et al, 2014), assim como Salmonella (Tejada et al, 2016). Nos aviários 5 e 8, Campylobacter foi isolado tanto de aves silvestres como de frangos, mas não possível realizar a comparação entre essas cepas para verificar a sua similaridade.…”

“…O outro foi identificado somente quanto ao sorogrupo, denominado O:3,10, por não ter sido detectado o antígeno flagelar. Tejada et al (2016), ao estudarem frangos na mesma região deste trabalho, observaram maior ocorrência do sorotipo Schwarzengrund, seguido de Mbandaka. Os sorotipos Enteritidis e Typhimurium parecem não ser tão frequentes em frangos no sul do Rio Grande do Sul, como são em outros locais.…”

Effectiveness of six molecular typing methods as epidemiological tools for the study of Salmonella isolates in two Colombian regions

“…Genotyping methods are useful tools for the retrospective identification of epidemic clones of Salmonella and subsequently tracking their dissemination throughout human and animal populations [25] as well as in the design of programs for control of disease, mainly for foodborne pathogens. In this study, RAPD and BOX methods were used as molecular tools to characterize Salmonella strains isolated from poultry and stool samples of patients with gastroenteritis (Table-1).…”