Experimental Coinfection of Chicken Anemia Virus and Mycoplasma gallisepticum Vaccine Strains in Broiler Chicks

Prezotto, Carolina Fontes; Marin, S. Y.; Araújo, Thiago Santos de; Barbosa, Fernanda de Oliveira; Barrios, Priscilla Rochele; Gomes, André M. O.; Peconick, Ana Paula; Resende, Maria Aparecida de; Sousa, Raimundo Vicente de; Martins, Nélson Rodrigo da Silva

doi:10.1590/1806-9061-2016-0235

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…There were multifocal areas of lymphoreticular infiltration in the liver. Our histopathological findings were in accordance with Prezotto et al (2016) stated in broiler chickens (Fig 1 and 2). Further, DNA was extracted from formalin fixed tissue samples viz.…”

Section: Resultssupporting

confidence: 91%

Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken

Ponmurugan

Reetha

Sasikala

et al. 2018

IJAR

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The present study was carried out to identify avian neoplastic viruses in the formalin fixed tissues of fancy chicken. The tissue samples collected during necropsy were examined by histopathology. It showed lymphoid and reticular cell infiltration in kidney, liver and lungs. For identification and differentiation of avian neoplastic viruses, PCR was performed using primer sets specific for Mareks disease virus, avian leukosis complex and reticuloendotheliosis virus. It was found that tumors were REV originated. Further confirmation, purified PCR product was subjected to sequencing. It showed 99% homology with other REV isolates available in the NCBI database. The present communication describes infection of a fancy chicken with REV on the basis of histopathological findings as well as molecular methods.

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Section: Resultssupporting

confidence: 91%

Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken

Ponmurugan

Reetha

Sasikala

et al. 2018

IJAR

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“…Also, proliferation of reticular cells and fibers associated with lymphatic depletion was observed in the spleen. Similar pathological findings were previously reported by Prezotto et al (2016) in broiler chickens. To the authors' knowledge, this is the first report of ALV infection associated with REV in the peafowl, which belong the Phasianidae family.…”

Section: Naturally Occurring Co-infection Of Avian Leukosis Virus (Susupporting

confidence: 91%

“…Zheng et al (2006) observed that an increase in the incidence of MD in broilers was associated with a simultaneous increase in the incidence of REV in chickens. Some researchers recently reported simultaneous endemic infections with ALV subgroup J and REV in commercial and local breeds of chickens in China (Cui et al, 2009).Another recent study also demonstrated the synergic pathological effects of the co-infection of CAV-MG (Mycoplasma gallisepticum) vaccine strains on experimentally-infected young SPF broiler chickens (Prezotto et al, 2016) based on pathology and molecular methods, like the present study. In the present study, it was not clear which virus first infected the birds.…”

Section: Naturally Occurring Co-infection Of Avian Leukosis Virus (Susupporting

confidence: 79%

Naturally Occurring Co-infection of Avian Leukosis Virus (subgroups A-E) and Reticuloendotheliosis Virus in Green Peafowls (Pavo muticus)

Khordadmehr

Firouzamandi

Zehtab-Najafi

et al. 2017

Rev. Bras. Cienc. Avic.

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Most of the neoplastic diseases affecting poultry have a viral etiology, such as Marek's disease, avian leukosis/sarcoma and reticuloendotheliosis. In the present paper, histopathology and molecular methods were performed, in addition of necropsy and gross morphology evaluation, for the differential diagnosis of these diseases in two green peafowls (Pavo muticus). The affected birds showed nonspecific signs, such as depression, anorexia, and diarrhea. At necropsy, diffuse enlargement by several times the normal size of the liver and the spleen was observed. In one of the birds, the liver presented white to grayish discoloration was present, and focal and nodular growths in the other bird. Grossly, the other visceral organs and the peripheral nerves were normal. Microscopically, most of the tumor cells were large and uniform with pleomorphic nuclei. The PCR analysis for Marek's disease virus (MDV), avian leukosis virus (ALV), lymphoproliferative disease virus (LPDV), and reticuloendotheliosis virus (REV)based on their specific sequences showed the presence of ALV subgroups A-E and REV. According to these results, definitive diagnosis was co-infection of ALV and REV in naturally-infected green peafowls.

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“…Recently, multiple vaccines are used (mainly Del-Ros, Nobilis P4, and Cux-1 strains) for breeder flocks producing maternal antibodies to newly born chickens which give protection till two to three weeks old (Haridy et al, 2009;Prezotto et al, 2016). The phylogenetic analysis and A.A. identity showed that the field viruses were distinct from the vaccines' strains as previously recorded by AboElkhair et al (2014) and Erfan et al (2018).…”

Section: Discussionmentioning

confidence: 80%

Molecular Characterization of Chicken Anaemia Virus Circulating in Commercial Poultry Flocks in Egypt during 2020

Abdelhalim¹,

Samir²,

Yehia³

2021

WVJ

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Chicken Anemia Virus (CAV) is an extremely contagious immunosuppressive disease causing high economic losses in poultry production. In the present study, tissue samples (bone marrow, thymus, and spleen) were collected from 86 different broiler chicken farms located in fourteen governorates in Egypt during 2020. They suffered from retard growth, weakness, and a drop in egg production with an observed mortality rate ranged 5-15%. A total of 26 samples were positive for CAV using PCR in six governorates in Lower Egypt with a 30% incidence rate, especially in Sharkia (78%), Ismailia (62.5%), and Alexandria (60%). The viral protein1 (VP1) gene of CAV was genetically characterized by sequencing of 10 selected viruses in six governorates. revealing that all Egyptian strains were clustered into two groups (A, B) that was distinct from vaccine strains (Del-Ros, Cux-1, and 26PA) which were clustered in group C. The seven Egyptian viruses in this study (A-Egypt-AN1-2020 to A-Egypt-AN7-2020) were clustered with the viruses from Japan, Argentina, and Malaysia in group A, and the other three viruses (A-Egypt-AN8-2020, A-Egypt-AN9-2020, A-Egypt-AN10-2020) were clustered with the viruses from Nigeria, and India in group B. The Egyptian viruses in the current study acquired new specific mutations clustering them into new subgroups (2A, 2B). By mutation analysis comparing with Del-Rose reference strains, V75I, M97L, and K139Q, E144Q were recorded in all viruses in the group A and B. All Egyptian viruses in the current study had specific new mutations at Y13N, H22N. Moreover, mutation at G74E in Egyptian viruses recorded in the current study was related to sub group 2A, I83V in three strains (A/Egypt/AN1/2020, A/Egypt/AN2/2020, A/Egypt/AN4/2020), and S140A in the hypervariable region was found in four strains (A/Egypt/AN1/2020, A/Egypt/AN2/2020, A/Egypt/AN4/2020 and A/Egypt/AN5/2020) in subgroup 2A. Furthermore, Q139 and Q144 amino acid substitutions, which are important in viral replication, were observed in all viruses. The field viruses in the study were distinct from the vaccinal strains by phylogenetic analysis and A.A. identity. In conclusion, the CAV was continuously circulating in Egypt from different genotypes. It acquired new specific mutations clustering them in a new subgroup, and it was distinct from vaccinal strains. Therefore, it is important to conduct continuous monitoring on the genetic evolution of CAV and further studies on the pathogenicity of the virus and the vaccine efficacy.

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Experimental Coinfection of Chicken Anemia Virus and Mycoplasma gallisepticum Vaccine Strains in Broiler Chicks

Abstract: This study aimed at determining the clinical and pathological

Cited by 7 publications

References 25 publications

Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken

Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken

Naturally Occurring Co-infection of Avian Leukosis Virus (subgroups A-E) and Reticuloendotheliosis Virus in Green Peafowls (Pavo muticus)

Molecular Characterization of Chicken Anaemia Virus Circulating in Commercial Poultry Flocks in Egypt during 2020

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