Development and Application of Est-Ssr Markers in Quails

Abstract

Aiming at accelerating the application of molecular markers in the genetic improvement of quails, six EST-SSR markers were successfully developed using a bioinformatics method. Polymorphisms of three quail populations (Chinese yellow quail, China black quail and Korean quail) were detected. The results showed that there were 2-6 alleles in six EST-SSR markers. Mean polymorphism information contents of Chinese yellow quails, Chinese black quails and Korean quails were determined as 0.5451, 0.4962 and 0.4937, respectively. Average heterozygosity valuesof 0.6134, 0.5759 and 0.5613 were calculated. Among the six EST-SSR markers, three were highly polymorphic, and the other three were moderately polymorphic. The newly-developed six EST-SSR markers may be used to determine the genetic diversity of quails. The six EST-SSR markers identified were related to carbohydrate metabolism and melanin synthesis, but their specific mechanisms need to be further analyzed.

Discovery of novel genic-SSR markers from transcriptome dataset of an important non-human primate, Macaca fascicularis

“…The average PIC value of 0.431 for the 19 polymorphic loci validated in this study was comparable to those genic-SSRs developed for the Korean quail (mean PIC value = 0.494) 26 and crab (mean PIC value = 0.49) 24 . Although not all the 19 genic-SSR markers showed high polymorphism and PIC values, all showed the reproducibility and specificity highly desired in genotyping by PCR.…”

“…It also serves as an excellent sequence resource for marker development. Transcriptome sequencing coupled with established bioinformatic pipeline have been used effectively for high throughput identification of genic-SSR markers from various organisms 24–26 . Some of the tools used for SSR mining include MicroSatellite identification tool (MISA) 27 , FullSSR 28 and Genome-wide Microsatellite analysing tool package (GMATA) 29 .…”

Correlation Analysis between MyoG Gene Polymorphism and Carcass Characteristics of Egg quails

“…At present, quail breeding is becoming more and more popular in poultry and quail is smaller than other poultry, so quail can be used as a good new experimental animal. Quail can be used as a research animal in many subjects such as poultry reproduction, histology, nutrition, endocrinology, embryology, physiology, pharmacology etc (Zhang et al, 2013;Li et al, 2019, Bai et al, 2016a, 2016b.The experimental values of quail in teaching and scientific studies are increasing gradually (Bai et al,2016c(Bai et al, , 2016d(Bai et al, , 2017. Few studies on correlation analysis between MyoG and carcass characteristics of quail are available.…”

Comparative analysis on early growth and development of different egg-laying quails

“…Moreover, it is a good material for genetic experimentation. The experimental values of quail in teaching and scientific studies are increasing gradually (Song et al, 2000(Song et al, , 2003(Song et al, , 2004Pang et al,2001aPang et al, , 2001bBai et al,2016aBai et al, , 2016bBai et al, ,2016cBai et al, ,2016dBai et al, ,2017. In this paper, growth and development of China yellow quail, Korea quail and Beijing white quail under the same breeding conditions were studied.…”